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| Other Sizes |
| Targets |
4-Nitrophenyl stearate targets lipases and esterases, functioning as a chromogenic substrate for these enzymes. The compound is hydrolyzed by lipases and esterases to release 4-nitrophenol, which can be detected by its yellow color at alkaline pH. It is not intended to exert pharmacological effects. Its utility lies in its use as a substrate for enzyme activity assays.
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| ln Vitro |
4-Nitrophenyl stearate is a biochemical reagent that can be utilized in life science research as an organic compound or biological material.
In vitro, 4-Nitrophenyl stearate is hydrolyzed by lipases and esterases to release 4-nitrophenol. The rate of hydrolysis is measured spectrophotometrically at 405 nm, providing a measure of enzyme activity. The compound's activity is assessed in enzyme assays using purified lipases or esterases, or using biological samples containing these enzymes. |
| ln Vivo |
4-Nitrophenyl stearate is not used for in vivo pharmacological activity assessment as a drug compound. Its primary applications are as a chromogenic substrate for lipase and esterase assays. The compound is not intended to be administered for therapeutic purposes. Its use is limited to in vitro biochemical assays.
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| Enzyme Assay |
In vitro enzyme assays for 4-Nitrophenyl stearate typically involve incubating the compound with lipase or esterase enzymes in a buffered solution. The hydrolysis of the ester bond releases 4-nitrophenol, which is detected spectrophotometrically at 405 nm. The reaction is monitored over time to determine enzyme activity. Standard protocols for lipase and esterase assays are employed.
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| Cell Assay |
4-Nitrophenyl stearate is not used in cell-based assays as a test compound for pharmacological effects. Its primary applications are as a chromogenic substrate in enzyme assays. The compound may be used in cell-based assays to measure lipase or esterase activity in cell lysates or culture media. Standard cell culture conditions (37°C, 5% CO₂) with appropriate media are employed.
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| Animal Protocol |
4-Nitrophenyl stearate is not used in animal studies as a pharmacological agent. Its primary applications are as a chromogenic substrate for enzyme assays. The compound is not intended to be administered to animals for efficacy evaluations. Its use is limited to in vitro biochemical assays.
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| ADME/Pharmacokinetics |
4-Nitrophenyl stearate has a molecular weight of 405.57 g/mol and a molecular formula of C₂₄H₃₉NO₄. It is a white to off-white crystalline solid. The compound is used as a chromogenic substrate for lipase and esterase assays. It is typically stored under standard laboratory conditions, protected from moisture and light. It is soluble in organic solvents such as ethanol, methanol, and dichloromethane.
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| Toxicity/Toxicokinetics |
4-Nitrophenyl stearate is intended for research use only and is not approved for human therapeutic applications. As a research chemical, comprehensive toxicological data are not extensively documented in the publicly accessible literature. Standard safety precautions should be observed when handling this compound, including the use of appropriate personal protective equipment. The compound should be handled in well-ventilated areas with proper waste disposal procedures.
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| Additional Infomation |
4-Nitrophenyl stearate (CAS#: 14617-86-8) has a molecular formula of C₂₄H₃₉NO₄ and a molecular weight of 405.57 g/mol. It is a chromogenic substrate for lipases and esterases, releasing 4-nitrophenol upon hydrolysis. This compound is not a drug and has not undergone clinical trials.
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| Molecular Formula |
C24H39NO4
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|---|---|
| Molecular Weight |
405.57
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| Exact Mass |
405.288
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| CAS # |
14617-86-8
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| PubChem CID |
4386004
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| Appearance |
White to off-white solid powder
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| Density |
1.006g/cm3
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| Boiling Point |
508ºC at 760mmHg
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| Melting Point |
68-72ºC
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| Flash Point |
163.3ºC
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| Vapour Pressure |
1.94E-10mmHg at 25°C
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| Index of Refraction |
1.498
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| LogP |
8.284
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
18
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| Heavy Atom Count |
29
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| Complexity |
414
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| Defined Atom Stereocenter Count |
0
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| SMILES |
CCCCCCCCCCCCCCCCCC(=O)OC1=CC=C(C=C1)[N+](=O)[O-]
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| InChi Key |
HAIZAZONHOVLEK-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C24H39NO4/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-15-16-17-24(26)29-23-20-18-22(19-21-23)25(27)28/h18-21H,2-17H2,1H3
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| Chemical Name |
(4-nitrophenyl) octadecanoate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.4657 mL | 12.3283 mL | 24.6567 mL | |
| 5 mM | 0.4931 mL | 2.4657 mL | 4.9313 mL | |
| 10 mM | 0.2466 mL | 1.2328 mL | 2.4657 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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