| Size | Price | Stock | Qty |
|---|---|---|---|
| 100mg |
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| 250mg |
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| Other Sizes |
| Targets |
Rhodamine 6G perchlorate does not have a defined pharmacological target as it is a fluorescent dye rather than a therapeutic agent. The compound binds to various cellular components including mitochondria and membranes through electrostatic interactions. It is used as a fluorescent probe to study cellular uptake, membrane potential, and mitochondrial function. In research applications, it serves as a tracer for cell labeling and tracking studies rather than as a drug targeting specific receptors.
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| ln Vitro |
In vitro activity of rhodamine 6G perchlorate is assessed based on its fluorescent properties rather than pharmacological activity. The dye exhibits strong fluorescence with high quantum yield and photostability. It is used in cell staining protocols to label mitochondria and other cellular structures. The compound is evaluated for its fluorescence intensity, photobleaching resistance, and cellular uptake efficiency. Typical concentrations for cell staining range from nanomolar to low micromolar depending on the application.
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| ln Vivo |
No specific in vivo pharmacological activity data are available for rhodamine 6G perchlorate as it is a fluorescent dye rather than a therapeutic agent. The compound may be used in animal studies as a fluorescent tracer for imaging applications, including tracking of cells, monitoring of vascular permeability, and optical imaging of tissues. Dosing and administration protocols depend on the specific imaging application and approved animal protocols.
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| Enzyme Assay |
For non-cellular assays, rhodamine 6G perchlorate is characterized by its spectroscopic properties including absorption and emission spectra, quantum yield, and fluorescence lifetime. Typical protocols involve dissolving the dye in appropriate solvents (water, ethanol, or methanol) and measuring fluorescence using a spectrofluorometer. The dye can be used as a standard for fluorescence calibration and as a reference compound in spectroscopic studies. Purity is typically determined by HPLC.
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| Cell Assay |
For in vitro cell-based studies, rhodamine 6G perchlorate is dissolved in DMSO or aqueous buffer and diluted in cell culture medium to working concentrations. Cells are incubated with the dye for various time points (typically 15-60 minutes) at 37°C, washed, and analyzed by fluorescence microscopy, flow cytometry, or fluorescence spectroscopy. The dye accumulates in mitochondria and can be used to assess mitochondrial membrane potential and cell viability. Appropriate controls including unstained cells should be included.
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| Animal Protocol |
For in vivo animal studies, rhodamine 6G perchlorate can be administered via intravenous, intraperitoneal, or other routes depending on the imaging application. The dye is used as a fluorescent tracer for optical imaging studies. Dosing solutions should be prepared fresh using appropriate vehicles. The compound should be handled with care due to the perchlorate content. Dosing and administration should follow approved animal protocols and institutional guidelines for fluorescent tracer studies.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of rhodamine 6G perchlorate are limited as it is a fluorescent dye rather than a pharmaceutical. The compound has a molecular weight of 543.01 and is a bright pink to red crystalline powder. It is soluble in water, ethanol, and methanol. The dye exhibits strong fluorescence with excitation at 530 nm and emission at 555 nm. Storage: keep in a cool, dry place protected from light. The compound should be stored in a tightly sealed container away from moisture and incompatible materials.
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| Toxicity/Toxicokinetics |
Toxicological data for rhodamine 6G perchlorate indicate that the compound should be handled with care due to the perchlorate content, which can be hazardous. The dye is for research use only and not for human consumption. Standard laboratory safety practices should be followed when handling this compound. Appropriate personal protective equipment including gloves and safety glasses should be worn. Specific LD50 values and detailed toxicological profiles have not been extensively reported in the literature.
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| Additional Infomation |
Rhodamine 6G perchlorate (CAS 13161-28-9) is a fluorescent xanthene dye also known as rhodamine 6G perchlorate or rhodamine 6G (perchlorate salt). It is widely used as a fluorescent tracer, laser dye, staining agent, and spectroscopic standard. Applications include flow cytometry, fluorescence microscopy, cell labeling, mitochondrial staining, and optical imaging. The dye exhibits strong green-yellow fluorescence with high quantum yield. No clinical trials or therapeutic approvals exist; the product is exclusively for research purposes.
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| Molecular Formula |
C28H31CLN2O7
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|---|---|
| Molecular Weight |
543.01
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| Exact Mass |
542.182
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| CAS # |
13161-28-9
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| Related CAS # |
989-38-8 (Parent)
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| PubChem CID |
13651880
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| Appearance |
Typically exists as solid at room temperature
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| Density |
1.1631 (rough estimate)
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| Boiling Point |
603.1ºC at 760mmHg
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| Melting Point |
263-265ºC
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| Flash Point |
318.6ºC
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| Vapour Pressure |
1.68E-14mmHg at 25°C
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| Index of Refraction |
1.6000 (estimate)
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| LogP |
6.579
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
9
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| Rotatable Bond Count |
7
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| Heavy Atom Count |
38
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| Complexity |
982
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| Defined Atom Stereocenter Count |
0
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| SMILES |
CCNC1=CC2=C(C=C1C)C(=C3C=C(C)/C(=N/CC)/C=C3O2)C4=CC=CC=C4C(=O)OCC.Cl(=O)(=O)(=O)O
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| InChi Key |
HDAFVOZRAUFNQH-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C28H30N2O3.ClHO4/c1-6-29-23-15-25-21(13-17(23)4)27(19-11-9-10-12-20(19)28(31)32-8-3)22-14-18(5)24(30-7-2)16-26(22)33-25;2-1(3,4)5/h9-16,29H,6-8H2,1-5H3;(H,2,3,4,5)
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| Chemical Name |
ethyl 2-[3-(ethylamino)-6-ethylimino-2,7-dimethylxanthen-9-yl]benzoate;perchloric acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.8416 mL | 9.2079 mL | 18.4159 mL | |
| 5 mM | 0.3683 mL | 1.8416 mL | 3.6832 mL | |
| 10 mM | 0.1842 mL | 0.9208 mL | 1.8416 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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