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| Targets |
Corn steep liquor does not have a defined pharmacological target as it is a nutrient supplement rather than a drug. Its biological activity derives from its rich composition of vitamins, amino acids, minerals, and nitrogen sources that support microbial growth and metabolism. It is used as an alternative to yeast extract and as a nitrogen source in biochemical industries, promoting the production of secondary metabolites and mycelial growth in fungal cultures.
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| ln Vitro |
Corn steep liquor (1–6%; 14 days) boosts the synthesis of L and fairy compounds. mycelium sordida [1].
Corn steep liquor is not evaluated for in vitro pharmacological activity as it is a fermentation nutrient. In microbiological research, it enhances production of fine chemicals and mycelium in fungal liquid cultures. Studies typically use concentrations of 1-6% over 14-day incubation periods. The supplement supports high-density culture of microorganisms including E. coli and S. cerevisiae. |
| ln Vivo |
Corn steep liquor is not administered as a therapeutic agent in animal studies. Its applications are exclusively in microbiology and fermentation research as a low-cost nutrient medium component. It is used in fermentation media for the simultaneous saccharification and co-fermentation (SSCF) process and for optimization studies. The supplement supports fed-batch production of recombinant proteins and fermentative production of lactic acid.
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| Enzyme Assay |
Corn steep liquor is characterized by standard chemical and microbiological analysis rather than enzyme or receptor binding assays. Typical protocols involve preparing the liquor as a component of growth media at concentrations ranging from 1-6%. The material is analyzed for total solids content (approximately 50% w/w), pH (3.7-4.1), nitrogen content, amino acid profile, vitamin content, and mineral composition. Quality control includes microbiological testing for sterility.
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| Cell Assay |
Corn steep liquor is used in cell culture applications as a nutrient supplement for microbial and fungal cells rather than for mammalian cell studies. Typical protocols involve adding corn steep liquor to liquid culture media at concentrations of 1-6% and incubating cultures for up to 14 days. The supplement supports mycelial growth and secondary metabolite production. For fungal liquid cultures, it serves as an excellent source of organic nitrogen.
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| Animal Protocol |
Corn steep liquor is not used in animal studies as it is a microbiological culture supplement. The material is handled in laboratory settings for fermentation and microbial culture applications. Storage: should be kept at 2-8°C. The product is supplied as a suspension and is suitable for microbiological culture techniques. Standard laboratory safety practices should be followed when handling this material.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties are not applicable for corn steep liquor as it is a nutrient supplement rather than a pharmaceutical. The material contains approximately 50% (w/w) solids and is a viscous concentrate rich in vitamins, amino acids, minerals, and growth stimulants. Storage temperature: 2-8°C. The product is classified as a combustible liquid with WGK nwg (non-hazardous to water). Flash point is not applicable.
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| Toxicity/Toxicokinetics |
Corn steep liquor is generally recognized as safe for its intended use as a food industry by-product and microbiological nutrient. The substance has been identified for potential human health concerns assessment. Standard laboratory safety precautions should be followed. Personal protective equipment including eyeshields and gloves is recommended when handling the material. The product is for research use only and not for human consumption as a drug.
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| References |
[1]. Kobori H, et, al. Utilization of Corn Steep Liquor for the Production of Fairy Chemicals by Lepista sordida Mycelia. J Fungi (Basel). 2022 Dec 1;8(12):1269.
[2]. Babakhani S, et, al. Non-sterile corn steep liquor a novel, cost effective and powerful culture media for Sporosarcina pasteurii cultivation for sand improvement. J Appl Microbiol. 2021 Apr;130(4):1232-1244. |
| Additional Infomation |
Corn steep liquor (CAS 66071-94-1) is a by-product of the corn wet-milling industry with EC Number 266-113-4. It is an excellent source of organic nitrogen and is used as a nutritional additive in fungal liquid cultures. The supplement increases production of fine chemicals and mycelium. It is useful as an inexpensive alternative to peptone for various microbiological production methods. No clinical or therapeutic applications exist.
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| Molecular Weight |
0
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| CAS # |
66071-94-1
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| Appearance |
Yellow to brown solid powder
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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