| Size | Price | Stock | Qty |
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| 1g |
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| Other Sizes |
| Targets |
Nonyl β-D-glucopyranoside does not have a defined pharmacological target as it is a biochemical detergent and surfactant. The compound is used to solubilize and stabilize membrane proteins for structural and functional studies. As a non-ionic detergent, it interacts with lipid membranes and proteins through hydrophobic and hydrogen bonding interactions without denaturing the proteins. It is used in the preparation of micelles and liposomes for drug delivery applications.
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| ln Vitro |
In vitro activity of nonyl β-D-glucopyranoside is assessed in protein solubilization and stabilization assays. The compound is used to extract membrane proteins from lipid bilayers while maintaining their native conformation and activity. It is evaluated for its ability to form micelles, its critical micelle concentration, and its effectiveness in solubilizing various membrane proteins. The compound is also used in crystallization studies of membrane proteins.
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| ln Vivo |
No specific in vivo pharmacological activity data are available for nonyl β-D-glucopyranoside as it is a biochemical reagent. The compound is used primarily in in vitro biochemical and biophysical research applications. It is not administered to animals for pharmacological evaluation. Its applications are limited to laboratory research in membrane protein biochemistry and formulation science.
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| Enzyme Assay |
For non-cellular assays, nonyl β-D-glucopyranoside is characterized by standard analytical techniques including HPLC, NMR, and mass spectrometry to confirm identity and purity. The compound is evaluated for its critical micelle concentration, surface tension reduction, and protein solubilization efficiency. Typical protocols involve preparing solutions of the detergent in appropriate buffers and measuring its physical properties using tensiometry, light scattering, or fluorescence spectroscopy.
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| Cell Assay |
For in vitro cell-based studies, nonyl β-D-glucopyranoside may be used in cell lysis and membrane protein extraction protocols. Cells are lysed in buffers containing the detergent to solubilize membrane proteins for downstream analysis. The compound is typically used at concentrations above its critical micelle concentration. Cells are treated with the detergent-containing buffer and membrane proteins are extracted for further analysis. Appropriate controls should be included in the experimental design.
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| Animal Protocol |
Nonyl β-D-glucopyranoside is not used in animal studies as it is a biochemical reagent. The compound is handled in biochemistry and biophysics laboratories for in vitro applications. Storage: keep in a cool, dry place protected from light and moisture. The compound should be stored in a tightly sealed container. Standard handling procedures for detergents should be followed.
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| ADME/Pharmacokinetics |
Nonyl β-D-glucopyranoside has a molecular weight of 306.40 and molecular formula C15H30O6. It is a non-ionic detergent and surfactant. Storage: keep in a cool, dry place protected from light and moisture. The compound is stable under recommended storage conditions. The compound has a critical micelle concentration in the millimolar range typical for alkyl glucosides. Solubility: soluble in water and aqueous buffers.
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| Toxicity/Toxicokinetics |
Nonyl β-D-glucopyranoside is for research use only and not for human consumption. Standard laboratory safety practices should be followed when handling this chemical. As a detergent, it may cause skin and eye irritation. Appropriate personal protective equipment including gloves and safety glasses should be worn. Specific LD50 values and detailed toxicological profiles have not been extensively reported.
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| References |
[1]. Annette Meister, et al. The interaction of n-nonyl-β-d-glucopyranoside and sodium dodecyl sulfate with DMPC and DMPG monolayers studied by infrared reflection absorption spectroscopy. Phys. Chem. Chem. Phys., 2004,6, 5543-5550
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| Additional Infomation |
Nonyl β-D-glucopyranoside (CAS 69984-73-2) is a non-ionic detergent used for the solubilization and stabilization of membrane proteins. It is used in biochemical and biophysical research for membrane protein extraction, purification, and crystallization studies. The compound is also used in the preparation of micelles and liposomes. No clinical trials or therapeutic approvals exist.
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| Molecular Formula |
C15H30O6
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|---|---|
| Molecular Weight |
306.40
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| Exact Mass |
306.204
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| CAS # |
69984-73-2
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| Related CAS # |
104242-08-2
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| PubChem CID |
155448
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| Appearance |
White to off-white solid powder
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| Density |
1.2±0.1 g/cm3
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| Boiling Point |
465.3±45.0 °C at 760 mmHg
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| Melting Point |
70°C(lit.)
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| Flash Point |
235.2±28.7 °C
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| Vapour Pressure |
0.0±2.6 mmHg at 25°C
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| Index of Refraction |
1.514
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| LogP |
1.56
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| Hydrogen Bond Donor Count |
4
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| Hydrogen Bond Acceptor Count |
6
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| Rotatable Bond Count |
10
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| Heavy Atom Count |
21
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| Complexity |
263
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| Defined Atom Stereocenter Count |
5
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| SMILES |
O1[C@]([H])([C@@]([H])([C@]([H])([C@@]([H])([C@@]1([H])C([H])([H])O[H])O[H])O[H])O[H])OC([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H]
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| InChi Key |
QFAPUKLCALRPLH-UXXRCYHCSA-N
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| InChi Code |
InChI=1S/C15H30O6/c1-2-3-4-5-6-7-8-9-20-15-14(19)13(18)12(17)11(10-16)21-15/h11-19H,2-10H2,1H3/t11-,12-,13+,14-,15-/m1/s1
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| Chemical Name |
(2R,3S,4S,5R,6R)-2-(hydroxymethyl)-6-nonoxyoxane-3,4,5-triol
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.2637 mL | 16.3185 mL | 32.6371 mL | |
| 5 mM | 0.6527 mL | 3.2637 mL | 6.5274 mL | |
| 10 mM | 0.3264 mL | 1.6319 mL | 3.2637 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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