| Size | Price | Stock | Qty |
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| 1mg |
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| Other Sizes |
| Targets |
Photobiotin acetate does not have a defined pharmacological target as it is a biochemical labeling reagent. The compound is used to study protein interactions and enzymatic processes through photochemical crosslinking technology. It can label single-stranded DNA and linear or supercoiled double-stranded DNA. The arylazide group enables light-activated covalent binding to target molecules.
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| ln Vitro |
A biochemical reagent called photobiotin acetate salt can be utilized in life science research as an organic compound or biological material.
In vitro activity of photobiotin acetate is assessed in labeling and detection assays. The compound is used for protein patterning with a photoactivatable derivative of biotin. It is capable of labeling single-stranded DNA and linear or supercoiled double-stranded DNA. The labeling efficiency and specificity are evaluated in various biochemical assays including DNA-DNA hybridization, protein-protein interaction studies, and enzyme-linked detection systems. |
| ln Vivo |
No specific in vivo pharmacological activity data are available for photobiotin acetate as it is a biochemical labeling reagent. The compound is used exclusively in in vitro biochemical and molecular biology applications. It is not administered to animals for pharmacological evaluation. Its applications are limited to laboratory research for nucleic acid and protein labeling.
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| Enzyme Assay |
For non-cellular assays, photobiotin acetate is characterized by standard analytical techniques including HPLC, NMR, and mass spectrometry to confirm identity and purity. The compound is evaluated in photolabeling reactions where it is activated by light to form covalent bonds with target molecules. Typical protocols involve incubating the compound with nucleic acids or proteins, exposing to appropriate wavelengths of light, and detecting labeled products using streptavidin-based detection systems.
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| Cell Assay |
For in vitro cell-based studies, photobiotin acetate is used in cell labeling applications. Cells or cellular components are incubated with the compound and exposed to light to enable photochemical crosslinking with target molecules. The labeled molecules are then detected using streptavidin-conjugated detection reagents. The compound is typically dissolved in appropriate buffers and used at concentrations determined by the specific experimental protocol.
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| Animal Protocol |
Photobiotin acetate is not used in animal studies as it is a biochemical labeling reagent. The compound is handled in molecular biology and biochemistry laboratories for in vitro applications. Storage: -20°C. The compound should be stored in a sealed container protected from light and moisture. Standard handling procedures for photoactivatable compounds should be followed.
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| ADME/Pharmacokinetics |
Photobiotin acetate has a molecular weight of 593.70 and molecular formula C25H39N9O6S. Storage: -20°C. The compound is a photoactivatable biotin analog used for non-radioactive labeling of nucleic acids and proteins. The compound should be protected from light during storage and handling to prevent premature activation. Solubility: soluble in appropriate aqueous buffers and organic solvents.
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| Toxicity/Toxicokinetics |
Photobiotin acetate is for research use only and not for human consumption. Standard laboratory safety practices should be followed when handling this chemical. The compound contains an arylazide group that can form reactive intermediates upon light activation. Appropriate personal protective equipment including gloves and safety glasses should be worn. Specific LD50 values and detailed toxicological profiles have not been extensively reported.
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| Additional Infomation |
Photobiotin acetate (CAS 96087-38-6) is a photoactivatable biotin analogue used for non-radioactive labeling of nucleic acids and proteins. It is used in biological study for protein patterning with a photoactivatable derivative of biotin. Applications include DNA-DNA hybridization to determine genomic relatedness, protein interaction studies, and enzymatic process research. No clinical or therapeutic applications exist.
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| Molecular Formula |
C25H39N9O6S
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|---|---|
| Molecular Weight |
593.70
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| Exact Mass |
593.274
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| CAS # |
96087-38-6
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| PubChem CID |
16218863
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| Appearance |
Typically exists as solid at room temperature
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| LogP |
4.451
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| Hydrogen Bond Donor Count |
5
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| Hydrogen Bond Acceptor Count |
11
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| Rotatable Bond Count |
15
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| Heavy Atom Count |
41
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| Complexity |
854
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| Defined Atom Stereocenter Count |
3
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| SMILES |
C(=O)(O)C.C([C@@H]1SC[C@@H]2NC(N[C@H]12)=O)CCCC(=O)NCCCN(C)CCCNC1C=CC(N=[N+]=[N-])=CC=1[N+](=O)[O-]
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| InChi Key |
FFBLNTOMOSLSQM-AYEYRVMASA-N
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| InChi Code |
InChI=1S/C23H35N9O4S.C2H4O2/c1-31(12-4-10-25-17-9-8-16(29-30-24)14-19(17)32(35)36)13-5-11-26-21(33)7-3-2-6-20-22-18(15-37-20)27-23(34)28-22;1-2(3)4/h8-9,14,18,20,22,25H,2-7,10-13,15H2,1H3,(H,26,33)(H2,27,28,34);1H3,(H,3,4)/t18-,20-,22-;/m0./s1
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| Chemical Name |
5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]-N-[3-[3-(4-azido-2-nitroanilino)propyl-methylamino]propyl]pentanamide;acetic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.6844 mL | 8.4218 mL | 16.8435 mL | |
| 5 mM | 0.3369 mL | 1.6844 mL | 3.3687 mL | |
| 10 mM | 0.1684 mL | 0.8422 mL | 1.6844 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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