| Size | Price | Stock | Qty |
|---|---|---|---|
| 250g |
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| Other Sizes |
| Targets |
As a non-ionic surfactant, Tween 65 does not have a defined pharmacological target. It is used as an excipient in pharmaceutical formulations, functioning as an emulsifier, solubilizing agent, and wetting agent. It is used in the preparation of stable oil-in-water drug emulsions, as a solubilizing agent for essential oils and oil-soluble vitamins, and as a wetting agent in oral and parenteral suspension formulations.
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|---|---|
| ln Vitro |
No specific in vitro pharmacological activity data are available for Tween 65 as it is an excipient rather than a therapeutic agent. In pharmaceutical research, the compound is evaluated for its emulsifying properties, solubilization capacity, and compatibility with active pharmaceutical ingredients. Its activity is assessed in terms of formulation performance, stability, and safety rather than direct biological activity.
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| ln Vivo |
No specific in vivo pharmacological activity data have been documented for Tween 65 as it is a pharmaceutical excipient. The compound is used in drug formulations to improve the solubility, stability, and bioavailability of active pharmaceutical ingredients. Its in vivo effects are related to its role as a formulation component rather than as a therapeutic agent. It is widely used in oral and parenteral formulations.
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| Enzyme Assay |
For non-cellular assays, Tween 65 is characterized by standard analytical techniques including HPLC, FTIR, and surface tension measurements. The compound can be evaluated for its emulsifying properties, critical micelle concentration, and surface activity. Typical protocols involve preparing serial dilutions of the compound in aqueous buffer and measuring surface tension using a tensiometer. The compound is also evaluated for its solubilization capacity for various hydrophobic compounds.
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| Cell Assay |
For in vitro cell-based studies, Tween 65 may be used as a component of cell culture media or as a solubilizing agent for hydrophobic test compounds. The compound is typically dissolved in aqueous buffers or cell culture medium at appropriate concentrations. Cells are cultured under standard conditions and exposed to Tween 65-containing formulations to assess biocompatibility and potential cytotoxic effects. Appropriate controls should be included in the experimental design.
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| Animal Protocol |
For in vivo animal studies, Tween 65 may be used as an excipient in drug formulations for administration via various routes including oral and parenteral. The compound is typically formulated with active pharmaceutical ingredients to improve solubility and bioavailability. Dosing solutions should be prepared according to the specific formulation protocol. Standard animal welfare guidelines should be followed for all in vivo studies.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of Tween 65 are not well characterized as it is a pharmaceutical excipient. The compound has an exact mass of 1010.407. It is a hydrophilic non-ionic surfactant that is dispersible in fats, oils, and water. Flash point: 300°F (149°C). Storage: room temperature. The compound should be stored in a sealed container away from moisture and incompatible materials.
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| Toxicity/Toxicokinetics |
Tween 65 (Polysorbate 65) is generally regarded as safe for use as a pharmaceutical excipient at appropriate concentrations. The compound is for research and pharmaceutical use and should be handled with standard laboratory safety practices. Specific LD50 values and detailed toxicological profiles have been established for regulatory purposes. Appropriate personal protective equipment including gloves and safety glasses should be worn when handling.
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| Additional Infomation |
See also: Polysorbate 65 (note moved to).
Tween 65 (CAS 9005-71-4) is also known as Polysorbate 65, polyoxyethylene sorbitan tristearate, and polyoxyethylene 20 sorbitan tristearate. It has an E number of E436. Synonyms include Alkamuls PSTS-20, Crillet 35, Glycosperse TS-20, Liposorb TS-20, and Montanox 65. It is used as an emulsifier in pharmaceuticals, cosmetics, and food products. No therapeutic claims exist for the compound itself. |
| Exact Mass |
1010.407
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|---|---|
| CAS # |
9005-71-4
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| PubChem CID |
168009877
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| Appearance |
Typically exists as solid at room temperature
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| Density |
1.05 g/mL at 20℃
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| Flash Point |
149°C
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| Hydrogen Bond Donor Count |
4
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
71
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| Complexity |
1180
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
MTUNHHDLLVEYBS-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/3C15H16ClNO.C15H17NO/c2*1-8-5-9-6-13-10(12(9)7-14(8)16)3-2-4-11(13)15(17)18;1-8-5-9(16)6-13-10-3-2-4-11(15(17)18)14(10)7-12(8)13;1-9-5-6-10-8-14-11(13(10)7-9)3-2-4-12(14)15(16)17/h2*5,7,11H,2-4,6H2,1H3,(H2,17,18);5-6,11H,2-4,7H2,1H3,(H2,17,18);5-7,12H,2-4,8H2,1H3,(H2,16,17)
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| Chemical Name |
6-chloro-7-methyl-2,3,4,9-tetrahydro-1H-fluorene-1-carboxamide;6-chloro-8-methyl-2,3,4,9-tetrahydro-1H-fluorene-1-carboxamide;6-methyl-2,3,4,9-tetrahydro-1H-fluorene-1-carboxamide
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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