| Size | Price | |
|---|---|---|
| 500mg | ||
| 1g | ||
| Other Sizes |
| Targets |
This compound does not target specific biological receptors but functions through direct chelation of metal ions. As a multidentate ligand, it forms stable complexes with divalent and trivalent metal ions (e.g., calcium, magnesium, zinc, cobalt, copper, and iron) through its multiple carboxylate groups. This property makes it an effective tool for studying metal-protein interactions and developing metal affinity purification methods.
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|---|---|
| ln Vitro |
In vitro, aminocaproic nitrilotriacetic acid functions primarily through its metal-chelating properties. It forms stable complexes with various divalent metal ions, effectively sequestering these metal ions and preventing their involvement in unintended biochemical reactions. In biochemical research, it is used to remove or capture metal ions from complex samples and to study the role of metal ions in enzyme-catalyzed reactions.
|
| Enzyme Assay |
A common application of this compound involves immobilizing it onto solid supports for metal ion affinity chromatography. For instance, literature reports describe the development of AC-NTA functionalized magnetic beads for rapid and efficient metal chelate affinity purification. The typical protocol may involve chemically conjugating AC-NTA to magnetic beads or agarose gel surfaces, followed by loading with metal ions (such as Ni²⁺ or Co²⁺) to create metal chelate affinity media for capturing histidine-tagged proteins.
|
| References |
[1]. https://pubchem.ncbi.nlm.nih.gov/compound/29971403
|
| Molecular Formula |
C16H29N3O7
|
|---|---|
| Molecular Weight |
375.42
|
| Exact Mass |
375.201
|
| CAS # |
1043881-31-7
|
| PubChem CID |
29971403
|
| Appearance |
White to off-white solid at room temperature
|
| LogP |
1.257
|
| Hydrogen Bond Donor Count |
5
|
| Hydrogen Bond Acceptor Count |
9
|
| Rotatable Bond Count |
16
|
| Heavy Atom Count |
26
|
| Complexity |
455
|
| Defined Atom Stereocenter Count |
1
|
| SMILES |
C(CCC(=O)NCCCCC(C(=O)O)N(CC(=O)O)CC(=O)O)CCN
|
| InChi Key |
FHJGDBAWMRVKAB-LBPRGKRZSA-N
|
| InChi Code |
InChI=1S/C16H29N3O7/c17-8-4-1-2-7-13(20)18-9-5-3-6-12(16(25)26)19(10-14(21)22)11-15(23)24/h12H,1-11,17H2,(H,18,20)(H,21,22)(H,23,24)(H,25,26)/t12-/m0/s1
|
| Chemical Name |
(2S)-6-(6-aminohexanoylamino)-2-[bis(carboxymethyl)amino]hexanoic acid
|
| Synonyms |
Aminocaproic Nitrilotriacetic Acid; 1043881-31-7; (2S)-6-(6-aminohexanoylamino)-2-[bis(carboxymethyl)amino]hexanoic acid; (S)-2,2'-((5-(6-Aminohexanamido)-1-carboxypentyl)azanediyl)diacetic acid; AC-NTA;
|
| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.6637 mL | 13.3184 mL | 26.6368 mL | |
| 5 mM | 0.5327 mL | 2.6637 mL | 5.3274 mL | |
| 10 mM | 0.2664 mL | 1.3318 mL | 2.6637 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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