| Size | Price | Stock | Qty |
|---|---|---|---|
| 100mg |
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| Other Sizes |
| Targets |
G3-CNP targets α-amylase as its substrate. The compound is specifically designed to be cleaved by α-amylase, releasing 2-chloro-4-nitrophenol which can be detected colorimetrically. As a biochemical reagent, it does not have a pharmacological target but serves as a tool for measuring enzyme activity.
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|---|---|
| ln Vitro |
α-amylase cleaves G3-CNP (50 µL; 10 min) at 37°C to yield 2-chloro-4-nitrophenol [1].
In vitro activity of G3-CNP is assessed in enzyme activity assays. The compound (50 µL; 10 min) is incubated with α-amylase at 37°C, and the cleavage product 2-chloro-4-nitrophenol is measured at 405 nm. This colorimetric assay can be used to determine α-amylase activity in various biological samples. |
| ln Vivo |
No specific in vivo pharmacological activity data have been documented for G3-CNP as it is a chromogenic substrate and research reagent. The compound is not administered to animals for pharmacological evaluation. Its applications are limited to in vitro biochemical and diagnostic research.
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| Enzyme Assay |
For non-cellular assays, G3-CNP is used as a substrate in α-amylase activity assays. Typical protocols involve incubating the compound with enzyme samples in appropriate buffer at 37°C, then measuring the release of 2-chloro-4-nitrophenol spectrophotometrically at 405 nm. Purity: >95% by HPLC. Moisture: <2%. Free CNP: <0.01%.
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| Cell Assay |
For in vitro cell-based studies, G3-CNP is not typically used as a direct test compound in cell culture. The compound is used in biochemical enzyme assays and diagnostic applications to measure α-amylase activity. If handled in a biological laboratory context, standard safety precautions should be taken.
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| Animal Protocol |
For in vivo animal studies, G3-CNP is not typically administered as a test compound. The compound is used primarily in in vitro biochemical and diagnostic research. If used in animal studies for specific research purposes, it would be administered via appropriate routes with suitable formulations.
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| ADME/Pharmacokinetics |
G3-CNP has a molecular weight of 659.98 and molecular formula C24H34ClNO18. It appears as an off-white to pale yellow powder. Purity: >95% by HPLC. Moisture: <2%. Free CNP: <0.01%. Storage: should be stored under recommended conditions.
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| Toxicity/Toxicokinetics |
G3-CNP is for research use only and not for human consumption. Standard laboratory safety practices should be followed when handling this chemical. Appropriate personal protective equipment including gloves and safety glasses should be worn. Specific LD50 values and detailed toxicological profiles have not been extensively reported.
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| References |
[1]. Ninomiya K, et al. Suppressive Effect of the α-Amylase Inhibitor Albumin from Buckwheat (Fagopyrum esculentum Moench) on Postprandial Hyperglycaemia. Nutrients. 2018 Oct 15;10(10):1503.
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| Additional Infomation |
G3-CNP (CAS 118291-90-0) is also known as 2-chloro-4-nitrophenyl α-D-maltotrioside and CNP-G3. It is a chromogenic substrate for α-amylase used in colorimetric enzyme activity assays. The compound is widely used in clinical diagnostics and biochemical research for the measurement of α-amylase activity.
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| Molecular Formula |
C24H34CLNO18
|
|---|---|
| Molecular Weight |
659.98
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| Exact Mass |
659.146
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| CAS # |
118291-90-0
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| PubChem CID |
9939545
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| Appearance |
White to off-white solid powder
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| Density |
1.8±0.1 g/cm3
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| Boiling Point |
976.0±65.0 °C at 760 mmHg
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| Flash Point |
544.1±34.3 °C
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| Vapour Pressure |
0.0±0.3 mmHg at 25°C
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| Index of Refraction |
1.690
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| LogP |
-1.25
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| Hydrogen Bond Donor Count |
10
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| Hydrogen Bond Acceptor Count |
18
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| Rotatable Bond Count |
9
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| Heavy Atom Count |
44
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| Complexity |
934
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| Defined Atom Stereocenter Count |
15
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| SMILES |
OC[C@@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@@H](O[C@@H]2[C@@H](O)[C@H](O)[C@@H](CO)O[C@]2(C2=CC=C([N+]([O-])=O)C=C2Cl)O[C@H]2O[C@H](CO)[C@@H](O)[C@H](O)[C@H]2O)O1
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| InChi Key |
KMYYNUOXSFGLNX-XFNLHOCBSA-N
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| InChi Code |
InChI=1S/C24H34ClNO18/c25-8-3-7(26(37)38)1-2-9(8)39-22-18(35)15(32)20(11(5-28)41-22)44-24-19(36)16(33)21(12(6-29)42-24)43-23-17(34)14(31)13(30)10(4-27)40-23/h1-3,10-24,27-36H,4-6H2/t10-,11-,12-,13-,14+,15-,16-,17-,18-,19-,20-,21-,22+,23-,24-/m1/s1
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| Chemical Name |
(2R,3R,4S,5S,6R)-2-[(2R,3S,4R,5R,6R)-6-[(2R,3S,4R,5R,6R)-6-(2-chloro-4-nitrophenoxy)-4,5-dihydroxy-2-(hydroxymethyl)oxan-3-yl]oxy-4,5-dihydroxy-2-(hydroxymethyl)oxan-3-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O: 125 mg/mL (189.40 mM)
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.5152 mL | 7.5760 mL | 15.1520 mL | |
| 5 mM | 0.3030 mL | 1.5152 mL | 3.0304 mL | |
| 10 mM | 0.1515 mL | 0.7576 mL | 1.5152 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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