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| Other Sizes |
| Targets |
4-(Imidazol-1-yl)phenol does not have a defined pharmacological target as it is a biochemical reagent and signal enhancer. The compound is used as a phenolic enhancer in enhanced chemiluminescent (ECL) reactions, which are useful in diagnostic assays. Its primary function is to enhance the signal in HRP-luminol chemiluminescence immunoassays.
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| ln Vitro |
In vitro activity of 4-(Imidazol-1-yl)phenol is assessed in chemiluminescence assays. The compound serves as a highly potent signal enhancer in horseradish peroxidase (HRP)-luminol chemiluminescence immunoassays. Its activity is measured by the enhancement of chemiluminescent signal in diagnostic assays.
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| ln Vivo |
No specific in vivo pharmacological activity data have been documented for 4-(Imidazol-1-yl)phenol as a therapeutic agent. The compound is used as a biochemical reagent and signal enhancer in diagnostic assays. It is not administered to animals for pharmacological evaluation. Its applications are limited to in vitro diagnostic research.
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| Enzyme Assay |
For non-cellular assays, 4-(Imidazol-1-yl)phenol is characterized by standard analytical techniques including HPLC, NMR, and mass spectrometry to confirm identity and purity. The compound has a molecular weight of 160.17 and formula C₉H₈N₂O. LogP: 1.5779. Storage: keep in a cool, dry place protected from light and moisture. The compound is a solid at room temperature.
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| Cell Assay |
For in vitro cell-based studies, 4-(Imidazol-1-yl)phenol is not typically used as a direct test compound. It is used as a signal enhancer in diagnostic immunoassays. If handled in a biological laboratory context, standard safety precautions should be taken. The compound should be stored in a cool, dry place protected from light and moisture.
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| Animal Protocol |
For in vivo animal studies, 4-(Imidazol-1-yl)phenol is not typically administered as a test compound. It is used as a biochemical reagent and signal enhancer in in vitro diagnostic assays. If used in animal studies for specific research purposes, it would be administered via appropriate routes with suitable formulations.
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| ADME/Pharmacokinetics |
4-(Imidazol-1-yl)phenol has a molecular weight of 160.17 and formula C₉H₈N₂O. LogP: 1.5779. Storage: keep in a cool, dry place protected from light and moisture. The compound is a solid at room temperature. Solubility: soluble in common organic solvents. The compound is stable under recommended storage conditions.
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| Toxicity/Toxicokinetics |
4-(Imidazol-1-yl)phenol is for research use only and not for human consumption. Standard laboratory safety practices should be followed when handling this chemical. Appropriate personal protective equipment including gloves and safety glasses should be worn. Specific LD₅₀ values and detailed toxicological profiles have not been extensively reported.
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| References | |
| Additional Infomation |
4-(Imidazol-1-yl)phenol (CAS 10041-02-8) is also known as 1-(p-hydroxyphenyl)imidazole and 4-(1H-imidazol-1-yl)phenol. It is a highly potent signal enhancer in HRP-luminol chemiluminescence immunoassays. Applications include diagnostic assays and enhanced chemiluminescent reactions. No clinical trials or therapeutic approvals exist.
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| Molecular Formula |
C9H8N2O
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|---|---|
| Molecular Weight |
160.17
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| Exact Mass |
160.064
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| CAS # |
10041-02-8
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| PubChem CID |
82315
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| Appearance |
White to off-white solid powder
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| Density |
1.2 g/cm3
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| Boiling Point |
350.7ºC at 760 mmHg
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| Melting Point |
204-206 °C(lit.)
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| Flash Point |
165.9ºC
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| Vapour Pressure |
2.14E-05mmHg at 25°C
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| Index of Refraction |
1.62
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| LogP |
1.577
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
2
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| Rotatable Bond Count |
1
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| Heavy Atom Count |
12
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| Complexity |
144
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| Defined Atom Stereocenter Count |
0
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| SMILES |
C1=CC(=CC=C1N2C=CN=C2)O
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| InChi Key |
CYKCUAPYWQDIKR-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C9H8N2O/c12-9-3-1-8(2-4-9)11-6-5-10-7-11/h1-7,12H
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| Chemical Name |
4-imidazol-1-ylphenol
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 6.2434 mL | 31.2168 mL | 62.4337 mL | |
| 5 mM | 1.2487 mL | 6.2434 mL | 12.4867 mL | |
| 10 mM | 0.6243 mL | 3.1217 mL | 6.2434 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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