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| Other Sizes |
| Targets |
Diglycolic anhydride functions primarily through the formation of amide bonds with biomolecules. The reaction is facilitated by the nucleophilic attack of amines on the electrophilic carbonyl carbon of the anhydride. The resulting products can exhibit significant biological activity, particularly in antimicrobial and anticancer applications. The compound's ability to form covalent bonds with phosphate groups in DNA may underlie its antimicrobial properties. It has been used in the synthesis of compounds that inhibit prostate cancer cells. The compound is also used in proteomics research.
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| ln Vitro |
N,N-diethyl diglycol amide is created by reacting diethylamine with diglycol anhydride. It is a biochemical material that is helpful in proteomic research.
In vitro, diglycolic anhydride is used to react with diethylamine to prepare N,N-diethyldiglycolamide. It is a useful biochemical substance for proteomics research. The compound has been shown to inhibit prostate cancer cells and extract infectious disease-causing bacteria. It also exhibits antimicrobial properties, potentially due to its ability to form covalent bonds with phosphate groups in DNA. Cellular assays typically evaluate its antimicrobial or anticancer activity. The compound's reactivity with amines makes it valuable for bioconjugation applications. |
| ln Vivo |
In vivo data for diglycolic anhydride is limited, as it is primarily a research reagent. The compound is classified for research use only and is not intended for human or veterinary applications. However, biologically active compounds synthesized using this building block have been investigated for their therapeutic potential. The compound's antimicrobial and anticancer properties suggest potential for in vivo efficacy when incorporated into drug molecules. Specific in vivo data for the parent compound is not available in the public literature.
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| Enzyme Assay |
In vitro enzyme/receptor binding assays for diglycolic anhydride are not typically performed, as it is a reactive chemical used for synthesis rather than a receptor-targeting agent. The compound is used to react with amines to form amide bonds. A typical assay involves using the compound in chemical reactions to prepare biologically active compounds. The compound is typically dissolved in organic solvents such as DMSO or THF. The resulting products can be evaluated for antimicrobial or anticancer activity using standard biochemical assays. IC₅₀ or MIC values are calculated from dose-response curves.
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| Cell Assay |
Cellular assays for diglycolic anhydride typically evaluate the activity of compounds synthesized using this building block. A standard protocol involves culturing cancer cell lines (e.g., prostate cancer cells) or bacterial strains in appropriate growth medium. Cells are treated with synthesized compounds at varying concentrations. Cell viability is assessed using MTT or similar assays for anticancer activity. Antimicrobial activity is evaluated by measuring minimum inhibitory concentration or zone of inhibition. IC₅₀ or MIC values are calculated from dose-response curves.
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| Animal Protocol |
In vivo animal studies for diglycolic anhydride are not standard, as it is a research reagent rather than a therapeutic candidate. The compound is classified for research use only and is not intended for human or veterinary applications. Any animal studies involving this compound would typically be conducted on the final biologically active compounds synthesized using this building block rather than on the building block itself. The compound's primary value lies in its use as a synthetic intermediate.
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| ADME/Pharmacokinetics |
Pharmacokinetic data for diglycolic anhydride is limited, as it is primarily a research reagent. The compound has a molecular formula of C₄H₄O₄ and a molecular weight of approximately 116.07 g/mol. It is stored as a solid at room temperature. As an anhydride, the compound is reactive and will hydrolyze in aqueous environments. The compound's reactivity with amines makes it valuable for bioconjugation. Specific ADME data is not available in the public literature.
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| Toxicity/Toxicokinetics |
Toxicological data for diglycolic anhydride is limited, as it is primarily a research reagent. The compound is classified for research use only and is not intended for human or veterinary applications. As an anhydride, it may be a skin and respiratory irritant. Standard safety precautions include handling with appropriate personal protective equipment (gloves, lab coat, safety goggles) in a well-ventilated area. The compound should be stored in a dry, cool place away from moisture and incompatible materials. Acute toxicity data is not readily available in the public literature. As with all research chemicals, appropriate laboratory safety practices should be followed.
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| Additional Infomation |
Diglycolic anhydride (1,4-Dioxane-2,6-dione, CAS 4480-83-5) is a biochemical reagent with the molecular formula C₄H₄O₄. It is used in the synthesis of biologically active compounds and has antimicrobial and anticancer properties. The compound is classified as a research-use-only compound not intended for diagnostic or therapeutic purposes. It is available from multiple commercial suppliers in various pack sizes. No clinical trials or approved drug status exist for this compound as it is not a therapeutic agent. Its applications are limited to research and chemical synthesis.
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| Molecular Formula |
C4H4O4
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|---|---|
| Molecular Weight |
116.07
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| Exact Mass |
116.01
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| CAS # |
4480-83-5
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| PubChem CID |
78232
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| Appearance |
White to off-white solid powder
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| Density |
1.4±0.1 g/cm3
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| Boiling Point |
240-241 ºC
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| Melting Point |
90-95 ºC
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| Flash Point |
105.7±20.4 °C
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| Vapour Pressure |
0.0±0.5 mmHg at 25°C
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| Index of Refraction |
1.450
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| LogP |
-2.06
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
0
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| Heavy Atom Count |
8
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| Complexity |
114
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| Defined Atom Stereocenter Count |
0
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| SMILES |
O1C([H])([H])C(=O)OC(C1([H])[H])=O
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| InChi Key |
PIYNUZCGMLCXKJ-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C4H4O4/c5-3-1-7-2-4(6)8-3/h1-2H2
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| Chemical Name |
1,4-dioxane-2,6-dione
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
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| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 8.6155 mL | 43.0775 mL | 86.1549 mL | |
| 5 mM | 1.7231 mL | 8.6155 mL | 17.2310 mL | |
| 10 mM | 0.8615 mL | 4.3077 mL | 8.6155 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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