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2-Bromo-3-methoxypyridine is a drug intermediate used for the synthesis of the metabolite of Piroxicam and for the preparation of triazolopyrimidine derivatives as AXL receptor tyrosine kinase function inhibitors. As a synthetic intermediate, it does not have specific biological targets. The brominated methoxypyridine scaffold allows for various chemical transformations, including cross-coupling reactions, ether cleavage, and nucleophilic substitution. The methoxy group influences the compound's electronic properties. The compound's derivatives may interact with AXL kinase and other biological targets.
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| ln Vitro |
In vitro, 2-bromo-3-methoxypyridine is used as a biochemical reagent and drug intermediate. It is used in the preparation of triazolopyrimidine derivatives and analogs as AXL receptor tyrosine kinase function inhibitors and for the synthesis of the metabolite of Piroxicam. Cellular assays are typically performed on the final compounds synthesized from this building block. The compound's brominated methoxypyridine scaffold allows for various chemical transformations, making it valuable for drug discovery and chemical synthesis.
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| ln Vivo |
In vivo data for 2-bromo-3-methoxypyridine is limited, as it is primarily a research reagent and synthetic intermediate. The compound is classified for research use only and is not intended for human or veterinary therapeutic applications. However, AXL kinase inhibitors and Piroxicam metabolites synthesized using this building block have been investigated for their biological activities. The compound's primary value lies in its use as a synthetic intermediate. Specific in vivo data for the parent compound is not available.
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| Enzyme Assay |
In vitro enzyme assays for 2-bromo-3-methoxypyridine are not typically performed, as it is a synthetic intermediate. The compound is used as a building block in the synthesis of AXL kinase inhibitors and Piroxicam metabolites. A typical assay involves using the compound in chemical reactions, including cross-coupling reactions and ether cleavage. The compound is dissolved in organic solvents such as DMSO or THF. Kinase inhibition assays with purified AXL kinase or other targets can be performed to evaluate the activity of the final synthesized compounds. IC₅₀ values are calculated from dose-response curves.
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| Cell Assay |
Cellular assays for 2-bromo-3-methoxypyridine derivatives typically evaluate AXL kinase inhibitory activity or other biological activities. A standard protocol involves culturing cancer cell lines in growth medium at 37°C with 5% CO₂. Cells are treated with synthesized compounds at varying concentrations for 24-72 hours. Cell viability is assessed using MTT or similar assays. AXL kinase inhibition is evaluated by measuring phosphorylation of downstream targets by Western blot or ELISA. IC₅₀ values are calculated from dose-response curves.
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| Animal Protocol |
In vivo animal studies for 2-bromo-3-methoxypyridine are not standard, as it is a synthetic intermediate rather than a therapeutic candidate. The compound is classified for research use only and is not intended for human or veterinary applications. Any animal studies would typically be conducted on the final AXL kinase inhibitors or other compounds synthesized using this building block. These studies would evaluate efficacy, pharmacokinetics, and safety in appropriate animal models. The compound's primary value lies in its use as a synthetic intermediate.
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| ADME/Pharmacokinetics |
Pharmacokinetic data for 2-bromo-3-methoxypyridine is limited, as it is primarily a research reagent. The compound has a molecular weight of 188.02 g/mol and a molecular formula of C₆H₆BrNO. It is a solid at room temperature with a melting point of 45-49°C and is stored in a dry, cool place. As a brominated methoxypyridine, it may undergo metabolic dehalogenation, O-demethylation, and oxidation. Specific ADME data is not available.
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| Toxicity/Toxicokinetics |
2-Bromo-3-methoxypyridine is classified for research use only and is not intended for human or veterinary applications. Standard safety precautions include handling with appropriate personal protective equipment (gloves, lab coat, safety goggles) in a well-ventilated area. The compound should be stored in a dry, cool place away from incompatible materials. Acute toxicity data is not readily available in the public literature. As with all research chemicals, appropriate laboratory safety practices should be followed. No specific LD₅₀ values or detailed toxicological profiles are available in the public domain.
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| Additional Infomation |
2-Bromo-3-methoxypyridine (CAS 24100-18-3) is a biochemical reagent with the molecular formula C₆H₆BrNO. It is a drug intermediate used for the synthesis of the metabolite of Piroxicam and for the preparation of AXL receptor tyrosine kinase inhibitors. The compound is classified as a research-use-only compound not intended for diagnostic or therapeutic purposes. It is available from multiple commercial suppliers.
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| Molecular Formula |
C6H6BRNO
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| Molecular Weight |
188.02
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| Exact Mass |
186.963
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| CAS # |
24100-18-3
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| PubChem CID |
90364
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| Appearance |
Off-white to light yellow solid powder
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| Density |
1.5±0.1 g/cm3
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| Boiling Point |
233.4±20.0 °C at 760 mmHg
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| Melting Point |
45-49 °C(lit.)
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| Flash Point |
95.0±21.8 °C
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| Vapour Pressure |
0.1±0.4 mmHg at 25°C
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| Index of Refraction |
1.543
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| LogP |
1.68
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
2
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| Rotatable Bond Count |
1
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| Heavy Atom Count |
9
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| Complexity |
89.1
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| Defined Atom Stereocenter Count |
0
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| SMILES |
COC1=CC=CN=C1Br
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| InChi Key |
PDOWLYNSFYZIQX-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C6H6BrNO/c1-9-5-3-2-4-8-6(5)7/h2-4H,1H3
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| Chemical Name |
2-bromo-3-methoxypyridine
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.3186 mL | 26.5929 mL | 53.1858 mL | |
| 5 mM | 1.0637 mL | 5.3186 mL | 10.6372 mL | |
| 10 mM | 0.5319 mL | 2.6593 mL | 5.3186 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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