| Size | Price | |
|---|---|---|
| Other Sizes |
| Targets |
Ethyl stearate does not have specific biological receptors as its primary targets. As a fatty acid ester, it can be hydrolyzed to stearic acid and ethanol, which are metabolized through normal lipid metabolism pathways. Stearic acid is a saturated fatty acid that can be incorporated into cell membranes and used for energy production. The compound has been reported to disrupt the cell cycle and induce apoptosis in certain cell types. It may interact with lipid metabolism enzymes and cellular membranes.
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|---|---|
| ln Vitro |
In vitro, ethyl stearate is used as a biochemical reagent and as an emollient, solubilizer for cosmetics, and flavoring agent. It has been reported to disrupt the cell cycle and induce apoptosis. The compound is used in studies of lipid metabolism, cell membrane biology, and apoptosis. Cellular assays typically evaluate its effects on cell proliferation, cell cycle progression, and apoptosis. The compound's fatty acid ester structure allows it to be incorporated into cellular membranes and lipid droplets.
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| ln Vivo |
In vivo, ethyl stearate is a long-chain fatty acid ethyl ester that can be metabolized to stearic acid and ethanol. Stearic acid is a common dietary fatty acid with various physiological roles. The compound is used as a flavoring agent in foods and pharmaceuticals and as an emollient in cosmetics. It is also used as a dehydrating agent for grapes to produce raisins. The compound is classified for research use and as a food additive. Specific therapeutic in vivo data is limited.
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| Enzyme Assay |
In vitro enzyme assays for ethyl stearate typically evaluate its hydrolysis by esterases or lipases. A standard assay protocol involves incubating the compound with purified enzymes or tissue homogenates in appropriate buffer systems. The compound is dissolved in organic solvents such as ethanol or DMSO and diluted to working concentrations. Enzyme activity is measured by quantifying the release of stearic acid or ethanol using colorimetric, fluorometric, or chromatographic methods. IC₅₀ or kinetic parameters are calculated from dose-response curves.
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| Cell Assay |
Cellular assays for ethyl stearate typically evaluate its effects on cell cycle and apoptosis. A standard protocol involves culturing cancer cell lines in growth medium at 37°C with 5% CO₂. Cells are treated with varying concentrations of ethyl stearate for 24-72 hours. Cell viability is assessed using MTT or similar assays. Cell cycle analysis is performed using flow cytometry with propidium iodide staining. Apoptosis is evaluated using Annexin V/PI staining or caspase activity assays. IC₅₀ values are calculated from dose-response curves.
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| Animal Protocol |
In vivo animal studies for ethyl stearate are limited, as it is primarily a food additive and cosmetic ingredient. The compound is generally recognized as safe for use in foods and cosmetics. Any animal studies would be limited to toxicological evaluations or studies on its metabolism as a dietary fat. The compound's primary value lies in its use as a flavoring agent, emollient, and research chemical. Specific in vivo protocols are not available in the public literature.
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| ADME/Pharmacokinetics |
Pharmacokinetic data for ethyl stearate indicates that it is hydrolyzed to stearic acid and ethanol in the gastrointestinal tract or by serum esterases. The compound has a molecular weight of 312.53 g/mol and a molecular formula of C₂₀H₄₀O₂. It is a solid at room temperature with a melting point of 35-38°C and is insoluble in water but soluble in alcohol and ether. Stearic acid is absorbed and metabolized through beta-oxidation. Ethanol is metabolized through alcohol dehydrogenase. Specific ADME data is limited.
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| Toxicity/Toxicokinetics |
Ethyl stearate is generally recognized as safe for use in foods and cosmetics. The compound is classified for research use and as a food additive. Standard safety precautions include handling with appropriate personal protective equipment (gloves, lab coat, safety goggles) in a well-ventilated area. The compound should be stored in a dry, cool place away from incompatible materials. Acute toxicity data is limited. No significant toxicity is expected at normal usage levels.
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| Additional Infomation |
Ethyl stearate is an octadecanoate ester formed by the condensation of the carboxyl group of octadecanoic acid (stearic acid) with the hydroxyl group of ethanol. It is a plant metabolite. It is a long-chain fatty acid ethyl ester and octadecanoate ester. Ethyl stearate has been reported in Mandragora autumnalis, Mitracarpus hirtus, and other organisms with relevant data.
Ethyl stearate (Stearic acid ethyl ester, CAS 111-61-5) is a biochemical reagent with the molecular formula C₂₀H₄₀O₂. It is a long-chain fatty acid ethyl ester used as an emollient, solubilizer for cosmetics, and flavoring agent. The compound has been reported to disrupt the cell cycle and induce apoptosis. It is classified as a research-use-only compound and as a food additive. It is available from multiple commercial suppliers. No clinical trials or approved drug status exist for this compound as it is not a therapeutic agent. |
| Molecular Formula |
C20H40O2
|
|---|---|
| Molecular Weight |
312.54
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| Exact Mass |
312.302
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| CAS # |
111-61-5
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| Related CAS # |
Ethyl stearate-d35;1219795-49-9
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| PubChem CID |
8122
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| Appearance |
White to off-white solid powder
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| Density |
0.9±0.1 g/cm3
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| Boiling Point |
356.0±0.0 °C at 760 mmHg
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| Melting Point |
33.4 °C
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| Flash Point |
161.0±7.5 °C
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| Vapour Pressure |
0.0±0.8 mmHg at 25°C
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| Index of Refraction |
1.446
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| LogP |
9.21
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
2
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| Rotatable Bond Count |
18
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| Heavy Atom Count |
22
|
| Complexity |
226
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| Defined Atom Stereocenter Count |
0
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| SMILES |
O(C([H])([H])C([H])([H])[H])C(C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H])=O
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| InChi Key |
MVLVMROFTAUDAG-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C20H40O2/c1-3-5-6-7-8-9-10-11-12-13-14-15-16-17-18-19-20(21)22-4-2/h3-19H2,1-2H3
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| Chemical Name |
ethyl octadecanoate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 6.67 mg/mL (21.34 mM)
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.1996 mL | 15.9980 mL | 31.9959 mL | |
| 5 mM | 0.6399 mL | 3.1996 mL | 6.3992 mL | |
| 10 mM | 0.3200 mL | 1.5998 mL | 3.1996 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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