| Size | Price | |
|---|---|---|
| Other Sizes |
| Targets |
5-Hydroxyisoquinoline is a synthetic intermediate and biochemical reagent used in medicinal chemistry. As a building block, it does not have specific biological receptors as its primary targets. The compound is used as a reagent in the preparation of PARP inhibitors. The isoquinoline scaffold is a privileged structure in medicinal chemistry, and the hydroxyl group provides a handle for further derivatization. PARP inhibitors are important therapeutic agents for cancer treatment. The compound's primary applications are as a synthetic intermediate for drug discovery and as a biochemical reagent.
|
|---|---|
| ln Vitro |
In vitro, 5-hydroxyisoquinoline is used as a biochemical reagent and organic compound for biomedical research. It is used as a reagent in the preparation of PARP inhibitors. Cellular assays are typically performed on the final PARP inhibitors synthesized from this building block. The compound's isoquinoline scaffold allows for various chemical transformations, including O-alkylation, electrophilic aromatic substitution, and cross-coupling reactions, making it valuable for drug discovery and medicinal chemistry.
|
| ln Vivo |
In vivo data for 5-hydroxyisoquinoline is limited, as it is primarily a research reagent and synthetic intermediate. The compound is classified for research use only and is not intended for human or veterinary therapeutic applications. However, PARP inhibitors synthesized using this building block have been investigated for their therapeutic potential in cancer treatment. The compound's primary value lies in its use as a synthetic intermediate for preparing PARP inhibitors and other bioactive compounds. Specific in vivo data for the parent compound is not available in the public literature.
|
| Enzyme Assay |
In vitro enzyme assays for 5-hydroxyisoquinoline are not typically performed, as it is primarily a synthetic intermediate. The compound is used as a building block in the synthesis of PARP inhibitors. A typical assay involves using the compound in chemical reactions to prepare final compounds. The compound is dissolved in organic solvents such as DMSO or ethanol. The resulting products can be evaluated for biological activity. PARP inhibition assays with purified PARP enzyme can be performed on the final synthesized compounds. IC₅₀ values are calculated from dose-response curves.
|
| Cell Assay |
Cellular assays for 5-hydroxyisoquinoline derivatives typically evaluate PARP inhibitory activity. A standard protocol involves culturing cancer cell lines in growth medium at 37°C with 5% CO₂. Cells are treated with synthesized compounds at varying concentrations for 24-72 hours. Cell viability is assessed using MTT or similar assays. PARP inhibition is evaluated by measuring PARP activity in cell lysates or by assessing DNA damage repair. IC₅₀ values are calculated from dose-response curves.
|
| Animal Protocol |
In vivo animal studies for 5-hydroxyisoquinoline are not standard, as it is a synthetic intermediate rather than a therapeutic candidate. The compound is classified for research use only and is not intended for human or veterinary applications. Any animal studies would typically be conducted on the final PARP inhibitors synthesized using this building block. These studies would evaluate efficacy in cancer models, pharmacokinetics, and safety. The compound's primary value lies in its use as a synthetic intermediate.
|
| ADME/Pharmacokinetics |
Pharmacokinetic data for 5-hydroxyisoquinoline is limited, as it is primarily a research reagent. The compound has a molecular weight of 145.16 g/mol and a molecular formula of C₉H₇NO. It is a solid at room temperature. As a hydroxyisoquinoline, it may undergo metabolic conjugation (glucuronidation, sulfation) and oxidation. Specific ADME data is not available. The compound is stored in a dry, cool place.
|
| Toxicity/Toxicokinetics |
5-Hydroxyisoquinoline is classified for research use only and is not intended for human or veterinary applications. Standard safety precautions include handling with appropriate personal protective equipment (gloves, lab coat, safety goggles) in a well-ventilated area. The compound should be stored in a dry, cool place away from incompatible materials. Acute toxicity data is not readily available in the public literature. As with all research chemicals, appropriate laboratory safety practices should be followed. No specific LD₅₀ values or detailed toxicological profiles are available in the public domain.
|
| Additional Infomation |
5-Hydroxyisoquinoline (Isoquinolin-5-ol, CAS 2439-04-5) is a biochemical reagent with the molecular formula C₉H₇NO. It is used as a synthetic intermediate for PARP inhibitors and as a biochemical reagent for biomedical research. The compound is classified as a research-use-only compound not intended for diagnostic or therapeutic purposes. It is available from multiple commercial suppliers in various pack sizes. No clinical trials or approved drug status exist for this compound as it is not a therapeutic agent.
|
| Molecular Formula |
C9H7NO
|
|---|---|
| Molecular Weight |
145.16
|
| Exact Mass |
145.052
|
| CAS # |
2439-04-5
|
| PubChem CID |
30386
|
| Appearance |
Off-white to yellow solid powder
|
| Density |
1.3±0.1 g/cm3
|
| Boiling Point |
332.1±15.0 °C at 760 mmHg
|
| Melting Point |
213-215
|
| Flash Point |
154.6±20.4 °C
|
| Vapour Pressure |
0.0±0.7 mmHg at 25°C
|
| Index of Refraction |
1.691
|
| LogP |
1.22
|
| Hydrogen Bond Donor Count |
1
|
| Hydrogen Bond Acceptor Count |
2
|
| Rotatable Bond Count |
0
|
| Heavy Atom Count |
11
|
| Complexity |
138
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
O([H])C1=C([H])C([H])=C([H])C2C([H])=NC([H])=C([H])C=21
|
| InChi Key |
CSNXUYRHPXGSJD-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C9H7NO/c11-9-3-1-2-7-6-10-5-4-8(7)9/h1-6,11H
|
| Chemical Name |
isoquinolin-5-ol
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
|---|
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 6.8890 mL | 34.4448 mL | 68.8895 mL | |
| 5 mM | 1.3778 mL | 6.8890 mL | 13.7779 mL | |
| 10 mM | 0.6889 mL | 3.4445 mL | 6.8890 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
