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| Other Sizes |
| Targets |
Peptone from soya is a nutrient source and culture medium component that does not have specific biological receptors as its primary targets. Its mechanism of action is nutritional rather than pharmacological, providing essential nutrients for microbial and cell growth. The compound is a protein hydrolysate containing a rich composition of amino acids, peptides, nucleosides, minerals, and other constituents. It stimulates or regulates cyclic arachidonic acid biosynthesis and exhibits enteric activity in the jejunum of piglets through this mechanism.
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| ln Vitro |
In vitro, peptone from soya is used as a component of culture medium for microbiology, tissue culture, and fermentation applications. It provides needed sources of nitrogen, carbon, and other nutrients for cell growth. The compound stimulates or regulates cyclic arachidonic acid biosynthesis. It has been shown to achieve improved cell growth and optimized cell performance compared to other peptones suitable for cell culture. Cellular assays typically use the compound as a medium additive to support cell growth and maintenance.
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| ln Vivo |
In vivo, peptone from soya stimulates or regulates cyclic arachidonic acid biosynthesis and exhibits enteric activity in the jejunum of piglets through this mechanism. The compound is classified for research use and is not intended for human or veterinary therapeutic applications. Its primary applications are as a culture medium component for microbiology and cell culture. Specific in vivo data for therapeutic applications is limited. The compound's nutritional value makes it valuable for research and industrial applications.
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| Enzyme Assay |
In vitro assays for peptone from soya typically evaluate its effectiveness as a culture medium component. A standard protocol involves preparing culture media with varying concentrations of the peptone and culturing microorganisms or cell lines under appropriate conditions. Cell growth, viability, and productivity are assessed by measuring optical density, colony counting, or metabolic assays. The compound's ability to support cell growth and protein production is evaluated. The compound's endotoxin content is measured using LAL assays for cell culture applications.
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| Cell Assay |
Cellular assays for peptone from soya typically involve its use as a culture medium additive to support cell growth and maintenance. A standard protocol involves culturing mammalian cell lines or microbial strains in media containing the peptone at varying concentrations. Cell growth, viability, and morphology are assessed using standard assays. The compound's ability to support cell proliferation and protein expression is evaluated. The compound's effects on cell metabolism and productivity are assessed using appropriate assays.
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| Animal Protocol |
In vivo animal studies for peptone from soya are limited, as it is primarily used as a culture medium component. The compound has been shown to exhibit enteric activity in the jejunum of piglets through stimulation of cyclic arachidonic acid biosynthesis. Any animal studies would be limited to evaluating its nutritional effects or its use as a feed additive. The compound's primary value lies in its use as a culture medium component for microbiology and cell culture. Specific in vivo protocols are not available in the public literature.
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| ADME/Pharmacokinetics |
Pharmacokinetic data for peptone from soya is not applicable, as it is a complex mixture of peptides, amino acids, and other nutrients rather than a discrete chemical compound. It is a solid at room temperature and is soluble in water at 25 mg/mL. It is stored as a powder at -20°C for up to 3 years or at 4°C for up to 2 years, or in solvent at -80°C for up to 6 months. As a protein hydrolysate, it is digested and absorbed as amino acids and peptides.
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| Toxicity/Toxicokinetics |
Peptone from soya is generally considered safe for research use as a culture medium component. The compound is classified for research use only and is not intended for human or veterinary therapeutic applications. Standard safety precautions include handling with appropriate personal protective equipment (gloves, lab coat, safety goggles) in a well-ventilated area. The compound should be stored in a dry, cool place away from incompatible materials. Acute toxicity data is limited. As with all research chemicals, appropriate laboratory safety practices should be followed.
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| References |
[1]. Jager LP, et al. Enteropooling in piglets induced by soya-peptone mediated via an increased biosynthesis of prostanoids. Vet Res Commun. 1986 Sep;10(5):407-12.
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| Additional Infomation |
Peptone is a mixture of derived proteins or cleavage products produced by the partial hydrolysis of natural proteins by acid or enzymes. Peptone is readily soluble in water and cannot be precipitated by heating, alkali, or ammonium sulfate saturation. (Dorland, 28th edition)
See also: Paraffin (Petroleum), n-cell C5-20 (note moved to). Peptone from soya (Peptones, soybean, CAS 91079-46-8) is a biochemical reagent and protein hydrolysate extracted from soybean flour. It is commonly used as a component of culture medium for microbiology, tissue culture, and fermentation applications. It provides nitrogen, carbon, and other nutrients and stimulates cyclic arachidonic acid biosynthesis. It is classified as a research-use-only compound and is available from multiple commercial suppliers. |
| Exact Mass |
5000.64
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|---|---|
| CAS # |
91079-46-8
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| PubChem CID |
167312541
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| Appearance |
Light yellow to light brown solid powder
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| Hydrogen Bond Donor Count |
8
|
| Hydrogen Bond Acceptor Count |
72
|
| Rotatable Bond Count |
46
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| Heavy Atom Count |
370
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| Complexity |
7410
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| Defined Atom Stereocenter Count |
0
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| SMILES |
CCC1(OC(=C(C(=O)O1)C=CC=CC=C2C(=O)OC3(CCC4(CC3)OC(=O)C(=CC=CC=CC5=C(OC(OC5=O)(C)CC)[O-])C(=O)O4)OC2=O)[O-])C.CC1CCC(C2(C1)OC(=C(C(=O)O2)C=CC=CC=C3C(=O)OC4(CCC5(CC4)OC(=O)C(=CC=CC=CC6=C(OC7(CC(CCC7C)C)OC6=O)[O-])C(=O)O5)OC3=O)[O-])C.CC1(OC(=O)C(=CC=CC=CC2=C(OC3(CCC4(CC3)OC(=C(C(=O)O4)C=CC=CC=C5C(=O)OC(OC5=O)(C)C)[O-])OC2=O)[O-])C(=O)O1)C.C1CCC2(CC1)OC(=O)C(=CC=CC=CC3=C(OC4(CCC5(CC4)OC(=C(C(=O)O5)C=CC=CC=C6C(=O)OC7(CCCCC7)OC6=O)[O-])OC3=O)[O-])C(=O)O2.C1=CC=C(C=C1)C2=C(C=CC(=C2)[N+]3=CC=C(C=C3)C4=CC=[N+](C=C4)C5=CC(=C(C=C5)O)C6=CC=CC=C6)O.C1=CC=C(C=C1)C2=C(C=CC(=C2)[N+]3=CC=C(C=C3)C4=CC=[N+](C=C4)C5=CC(=C(C=C5)O)C6=CC=CC=C6)O.C1=CC=C(C=C1)C2=C(C=CC(=C2)[N+]3=CC=C(C=C3)C4=CC=[N+](C=C4)C5=CC(=C(C=C5)O)C6=CC=CC=C6)O.C1=CC=C(C=C1)C2=C(C=CC(=C2)[N+]3=CC=C(C=C3)C4=CC=[N+](C=C4)C5=CC(=C(C=C5)O)C6=CC=CC=C6)O
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| InChi Key |
DENDBKLJAQIVGF-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C44H48O16.C40H40O16.C36H36O16.4C34H24N2O2.C34H32O16/c1-25-15-17-27(3)43(23-25)57-37(49)31(38(50)58-43)13-9-5-7-11-29-33(45)53-41(54-34(29)46)19-21-42(22-20-41)55-35(47)30(36(48)56-42)12-8-6-10-14-32-39(51)59-44(60-40(32)52)24-26(2)16-18-28(44)4;41-29-25(30(42)50-37(49-29)17-9-3-10-18-37)13-5-1-7-15-27-33(45)53-39(54-34(27)46)21-23-40(24-22-39)55-35(47)28(36(48)56-40)16-8-2-6-14-26-31(43)51-38(52-32(26)44)19-11-4-12-20-38;1-5-33(3)45-25(37)21(26(38)46-33)13-9-7-11-15-23-29(41)49-35(50-30(23)42)17-19-36(20-18-35)51-31(43)24(32(44)52-36)16-12-8-10-14-22-27(39)47-34(4,6-2)48-28(22)40;4*37-33-13-11-29(23-31(33)27-7-3-1-4-8-27)35-19-15-25(16-20-35)26-17-21-36(22-18-26)30-12-14-34(38)32(24-30)28-9-5-2-6-10-28;1-31(2)43-23(35)19(24(36)44-31)11-7-5-9-13-21-27(39)47-33(48-28(21)40)15-17-34(18-16-33)49-29(41)22(30(42)50-34)14-10-6-8-12-20-25(37)45-32(3,4)46-26(20)38/h5-14,25-28,49,51H,15-24H2,1-4H3;1-2,5-8,13-16,45,47H,3-4,9-12,17-24H2;7-16,37,39H,5-6,17-20H2,1-4H3;4*1-24H;5-14,39,41H,15-18H2,1-4H3
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| Chemical Name |
3,12-bis[5-(2,2-dimethyl-4,6-dioxo-1,3-dioxan-5-ylidene)penta-1,3-dienyl]-4,13-dioxo-1,5,10,14-tetraoxadispiro[5.2.59.26]hexadeca-2,11-diene-2,11-diolate;3,12-bis[5-(2,4-dioxo-1,5-dioxaspiro[5.5]undecan-3-ylidene)penta-1,3-dienyl]-4,13-dioxo-1,5,10,14-tetraoxadispiro[5.2.59.26]hexadeca-2,11-diene-2,11-diolate;3-[5-[12-[5-(8,11-dimethyl-2-oxido-4-oxo-1,5-dioxaspiro[5.5]undec-2-en-3-yl)penta-2,4-dienylidene]-2,4,11,13-tetraoxo-1,5,10,14-tetraoxadispiro[5.2.59.26]hexadecan-3-ylidene]penta-1,3-dienyl]-8,11-dimethyl-4-oxo-1,5-dioxaspiro[5.5]undec-2-en-2-olate;2-ethyl-5-[5-[12-[5-(2-ethyl-2-methyl-4-oxido-6-oxo-1,3-dioxin-5-yl)penta-2,4-dienylidene]-2,4,11,13-tetraoxo-1,5,10,14-tetraoxadispiro[5.2.59.26]hexadecan-3-ylidene]penta-1,3-dienyl]-2-methyl-6-oxo-1,3-dioxin-4-olate;4-[4-[1-(4-hydroxy-3-phenylphenyl)pyridin-1-ium-4-yl]pyridin-1-ium-1-yl]-2-phenylphenol
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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