| Size | Price | Stock | Qty |
|---|---|---|---|
| 10g |
|
||
| Other Sizes |
| Targets |
Permanent Orange is a synthetic pigment that does not have specific biological receptors as its primary targets. As a pigment and coloring agent, its mechanism of action is physical rather than pharmacological, involving the absorption and reflection of light to produce color. The compound's azo chromophore is responsible for its orange color. The pigment's primary applications are in paints, coatings, plastics, and inks rather than as a therapeutic or pharmacological agent. Its use in research is primarily as a reference standard or for materials science applications.
|
|---|---|
| ln Vitro |
For life science-related research, Permanent Orange is a biochemical reagent that can be utilized as an organic compound or biological material.
In vitro, Permanent Orange is used as a biochemical reagent and synthetic pigment for life science research. It is widely used in the manufacture of paints and coatings. The pigment produces a bright orange color that is fade and weather resistant. Cellular assays are not typically performed with this compound as a direct cell-modulating agent, as its primary applications are in materials science and as a coloring agent. The compound's use in research is primarily as a reference standard for pigment analysis or in materials science applications. |
| ln Vivo |
In vivo data for Permanent Orange is limited, as it is primarily a pigment used in paints, coatings, and industrial applications rather than a therapeutic agent. The compound is classified for research use only and is not intended for human or veterinary therapeutic applications. Its primary applications are in the manufacture of paints, coatings, and other industrial products. Specific in vivo data for the parent compound is not available in the public literature. The compound is not a therapeutic candidate.
|
| Enzyme Assay |
In vitro assays for Permanent Orange typically evaluate its pigment properties rather than biological activity. A standard protocol involves dispersing the pigment in an appropriate medium and characterizing its color properties using spectrophotometry. The pigment's purity is assessed using UV-vis spectrophotometry, with a lambda max of 478.0-482.0 nm in CHCl₃. The pigment's stability, lightfastness, and weather resistance are evaluated using standard testing methods. The compound is used as a reference standard for pigment analysis in materials science and quality control applications.
|
| Cell Assay |
Cellular assays for Permanent Orange are not standard, as the compound is primarily used as a pigment in industrial applications rather than as a cell-modulating agent. The compound's azo structure suggests potential for cytotoxicity at high concentrations. Any cellular studies would focus on evaluating the compound's cytotoxicity or potential for DNA damage. A typical protocol would involve culturing appropriate cell lines in growth medium at 37°C with 5% CO₂. Cells would be treated with varying concentrations of the pigment. Cell viability would be assessed using MTT or similar assays.
|
| Animal Protocol |
In vivo animal studies for Permanent Orange are not standard, as it is primarily a pigment used in industrial applications rather than a therapeutic candidate. The compound is classified for research use only and is not intended for human or veterinary applications. Any animal studies would be limited to toxicological evaluations of the pigment as an industrial chemical. The compound's primary value lies in its use as a pigment in paints, coatings, and other products. Specific in vivo protocols are not available in the public literature.
|
| ADME/Pharmacokinetics |
Pharmacokinetic data for Permanent Orange is not applicable, as it is a pigment rather than a therapeutic agent. The compound has a molecular weight of 338.28 g/mol and a molecular formula of C₁₆H₁₀N₄O₅. It is a solid at room temperature with a melting point of 302°C. As a pigment, it is expected to have very low solubility and bioavailability. The compound is stored in a dry, cool place. Specific ADME data is not available.
|
| Toxicity/Toxicokinetics |
Permanent Orange is classified for research use only and is not intended for human or veterinary applications. Standard safety precautions include handling with appropriate personal protective equipment (gloves, lab coat, safety goggles) in a well-ventilated area. The compound should be stored in a dry, cool place away from incompatible materials. Acute toxicity data is not readily available in the public literature. As with all research chemicals, appropriate laboratory safety practices should be followed. No specific LD₅₀ values are available in the public domain.
|
| Additional Infomation |
According to the U.S. Food and Drug Administration (FDA), D&C Orange 17 may cause cancer.
Permanent Orange (Pigment Orange 5, CI 12075, CAS 3468-63-1) is a synthetic pigment with the molecular formula C₁₆H₁₀N₄O₅. It produces a bright orange color that is fade and weather resistant and is widely used in the manufacture of paints and coatings. The compound is classified as a research-use-only compound not intended for diagnostic or therapeutic purposes. It is available from multiple commercial suppliers in various pack sizes. |
| Molecular Formula |
C16H10N4O5
|
|---|---|
| Molecular Weight |
338.27
|
| Exact Mass |
338.065
|
| CAS # |
3468-63-1
|
| PubChem CID |
18981
|
| Appearance |
Orange to red solid powder
|
| Density |
1.5±0.1 g/cm3
|
| Boiling Point |
597.9±50.0 °C at 760 mmHg
|
| Melting Point |
302 °C
; 302 °C
|
| Flash Point |
315.4±30.1 °C
|
| Vapour Pressure |
0.0±1.8 mmHg at 25°C
|
| Index of Refraction |
1.712
|
| LogP |
5.09
|
| Hydrogen Bond Donor Count |
1
|
| Hydrogen Bond Acceptor Count |
7
|
| Rotatable Bond Count |
2
|
| Heavy Atom Count |
25
|
| Complexity |
532
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
C1=CC=C2C(=C1)C=CC(=C2N=NC3=C(C=C(C=C3)[N+](=O)[O-])[N+](=O)[O-])O
|
| InChi Key |
HBHZKFOUIUMKHV-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C16H10N4O5/c21-15-8-5-10-3-1-2-4-12(10)16(15)18-17-13-7-6-11(19(22)23)9-14(13)20(24)25/h1-9,21H
|
| Chemical Name |
1-[(2,4-dinitrophenyl)diazenyl]naphthalen-2-ol
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.9562 mL | 14.7811 mL | 29.5622 mL | |
| 5 mM | 0.5912 mL | 2.9562 mL | 5.9124 mL | |
| 10 mM | 0.2956 mL | 1.4781 mL | 2.9562 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
