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| Other Sizes |
| Targets |
2-Amino-3-hydroxypyridine is a synthetic intermediate and chromogenic reagent rather than a direct pharmacological agent. As a building block, it does not have specific biological receptors as its primary targets. The compound's amino and hydroxyl groups allow for diverse chemical transformations, including complexation with transition metals. It has been used to synthesize functionalized coumarins and 1,4-oxazines. Its primary applications are as a chromogenic reagent for analytical chemistry, a corrosion inhibitor, and a synthetic intermediate for organic synthesis.
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| ln Vitro |
2-Amino-3-hydroxypyridine is employed as a chromogenic reagent in spectrophotometric osmium measurement.
In vitro, 2-amino-3-hydroxypyridine is used as a biochemical reagent and chromogenic reagent for the spectrophotometric determination of osmium. It is also used as a biomaterial or organic/chemical reagent for biomedical research. The compound forms complexes with transition metals and can be used in the synthesis of various heterocyclic compounds. Cellular assays are not typically performed with this compound as a direct cell-modulating agent, as its primary applications are in analytical chemistry and organic synthesis. |
| ln Vivo |
In vivo data for 2-amino-3-hydroxypyridine is limited, as it is primarily a research reagent and analytical reagent. The compound is classified for research use only and is not intended for human or veterinary therapeutic applications. Its primary applications are in analytical chemistry for osmium determination, corrosion inhibition, and organic synthesis. Specific in vivo data for the parent compound is not available in the public literature. The compound is not a therapeutic candidate.
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| Enzyme Assay |
In vitro assays for 2-amino-3-hydroxypyridine typically evaluate its use as a chromogenic reagent for osmium determination. A standard protocol involves preparing a solution of the compound in an appropriate buffer. Osmium-containing samples are added, and the development of color is measured spectrophotometrically at a specific wavelength. The compound's ability to form complexes with transition metals can be studied using UV-Vis spectroscopy. For corrosion inhibition studies, metal samples are immersed in acidic solutions containing varying concentrations of the compound, and corrosion rates are measured using electrochemical techniques or weight loss methods.
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| Cell Assay |
Cellular assays for 2-amino-3-hydroxypyridine are not standard, as the compound is primarily used as an analytical reagent and synthetic intermediate rather than as a cell-modulating agent. The compound's potential for metal complexation suggests possible cytotoxicity at high concentrations. Any cellular studies would focus on evaluating the compound's cytotoxicity. A typical protocol would involve culturing appropriate cell lines in growth medium at 37°C with 5% CO₂. Cells would be treated with varying concentrations of the compound. Cell viability would be assessed using MTT or similar assays.
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| Animal Protocol |
In vivo animal studies for 2-amino-3-hydroxypyridine are not standard, as it is primarily a research reagent and analytical reagent. The compound is classified for research use only and is not intended for human or veterinary applications. Any animal studies would be limited to toxicological evaluations of the compound as an industrial chemical. The compound's primary value lies in its use as a chromogenic reagent and synthetic intermediate. Specific in vivo protocols are not available in the public literature.
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| ADME/Pharmacokinetics |
Pharmacokinetic data for 2-amino-3-hydroxypyridine is limited, as it is primarily a research reagent. The compound has a molecular weight of 110.12 g/mol and a molecular formula of C₅H₆N₂O. It is a solid at room temperature with a melting point of 170-176°C. It is insoluble in water but soluble in alcohol and DMSO. As an aminopyridine, it may undergo metabolic conjugation and oxidation. Specific ADME data is not available. The compound is stored in a dry, cool place.
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| Toxicity/Toxicokinetics |
Toxicity Summary
It is safe at the current usage and concentration. Ingredient, concentration, and usage information can be found at: https://cir-reports.cir-safety.org 2-Amino-3-hydroxypyridine is classified for research use only and is not intended for human or veterinary applications. Standard safety precautions include handling with appropriate personal protective equipment (gloves, lab coat, safety goggles) in a well-ventilated area. The compound should be stored in a dry, cool place away from incompatible materials. Acute toxicity data is not readily available in the public literature. As with all research chemicals, appropriate laboratory safety practices should be followed. No specific LD₅₀ values are available in the public domain. |
| Additional Infomation |
2-Amino-3-hydroxypyridine is a monohydroxypyridine and also an aminopyridine.
2-Amino-3-hydroxypyridine (2-Amino-3-pyridinol, CAS 16867-03-1) is a biochemical reagent with the molecular formula C₅H₆N₂O. It is a heterocyclic compound featuring amino and hydroxyl groups on a pyridine ring. It is used as a chromogenic reagent for the spectrophotometric determination of osmium, forms complexes with transition metals, and inhibits corrosion of aluminium and copper in acidic solutions. The compound is classified as a research-use-only compound not intended for diagnostic or therapeutic purposes. |
| Molecular Formula |
C5H6N2O
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|---|---|
| Molecular Weight |
110.11
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| Exact Mass |
110.048
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| CAS # |
16867-03-1
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| PubChem CID |
28114
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| Appearance |
Off-white to light brown solid powder
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| Density |
1.3±0.1 g/cm3
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| Boiling Point |
385.2±27.0 °C at 760 mmHg
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| Melting Point |
168-172 °C(lit.)
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| Flash Point |
186.8±23.7 °C
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| Vapour Pressure |
0.0±0.9 mmHg at 25°C
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| Index of Refraction |
1.651
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| LogP |
0.77
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
0
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| Heavy Atom Count |
8
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| Complexity |
76.8
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| Defined Atom Stereocenter Count |
0
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| SMILES |
O([H])C1C([H])=C([H])C([H])=NC=1N([H])[H]
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| InChi Key |
BMTSZVZQNMNPCT-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C5H6N2O/c6-5-4(8)2-1-3-7-5/h1-3,8H,(H2,6,7)
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| Chemical Name |
2-aminopyridin-3-ol
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 9.0818 mL | 45.4091 mL | 90.8183 mL | |
| 5 mM | 1.8164 mL | 9.0818 mL | 18.1637 mL | |
| 10 mM | 0.9082 mL | 4.5409 mL | 9.0818 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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