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| Targets |
N,N'-Methylenebisacrylamide is a cross-linking agent and biochemical reagent rather than a direct pharmacological agent. As a reagent, it does not have specific biological receptors as its primary targets. Its mechanism of action is physical rather than pharmacological, involving the cross-linking of acrylamide polymers to form polyacrylamide gels. These gels are used for the electrophoretic separation of nucleic acids and proteins. Its primary applications are in molecular biology, biochemistry, and polymer chemistry.
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| ln Vitro |
In vitro, N,N'-methylenebisacrylamide is used as a cross-linking agent in the preparation of polyacrylamide gels for the electrophoretic separation of nucleic acids and proteins. It is used as an organic intermediate and cross-linking agent for making polyacrylamides. Cellular assays are not typically performed with this compound as a direct cell-modulating agent, as its primary applications are in gel electrophoresis and polymer chemistry. The compound's ability to form cross-linked polymers makes it valuable for molecular biology research.
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| ln Vivo |
In vivo data for N,N'-methylenebisacrylamide is limited, as it is primarily used as an in vitro research reagent. The compound is classified for research use only and is not intended for human or veterinary therapeutic applications. As a monomer, acrylamide derivatives can be neurotoxic and potentially carcinogenic. The compound's primary applications are in molecular biology and biochemistry rather than as a therapeutic agent. Specific in vivo data for the parent compound is not available in the public literature.
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| Enzyme Assay |
In vitro assays for N,N'-methylenebisacrylamide typically evaluate its use as a cross-linking agent in gel electrophoresis. A standard protocol involves mixing the compound with acrylamide in appropriate buffer solutions to prepare polyacrylamide gels. The compound is dissolved in water or buffer at concentrations ranging from 1-5% (w/v) of the total acrylamide content. Gel polymerization is initiated by the addition of ammonium persulfate and TEMED. The resulting gels are used for the electrophoretic separation of proteins or nucleic acids. Gel porosity and resolution are evaluated based on the separation of molecular weight markers.
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| Cell Assay |
Cellular assays for N,N'-methylenebisacrylamide are not standard, as the compound is primarily used as a reagent for gel electrophoresis rather than as a cell-modulating agent. The compound's acrylamide structure suggests potential for neurotoxicity and cytotoxicity at high concentrations. Any cellular studies would focus on evaluating the compound's cytotoxicity. A typical protocol would involve culturing appropriate cell lines in growth medium at 37°C with 5% CO₂. Cells would be treated with varying concentrations of the compound. Cell viability would be assessed using MTT or similar assays.
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| Animal Protocol |
In vivo animal studies for N,N'-methylenebisacrylamide are not standard, as it is primarily an in vitro research reagent. The compound is classified for research use only and is not intended for human or veterinary applications. Any animal studies would be limited to toxicological evaluations of the compound as an industrial chemical or potential neurotoxin. The compound's acrylamide structure suggests potential for neurotoxicity. Specific in vivo protocols are not available in the public literature.
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| ADME/Pharmacokinetics |
Pharmacokinetic data for N,N'-methylenebisacrylamide is not applicable, as it is a cross-linking reagent rather than a therapeutic agent. The compound has a molecular weight of 154.17 g/mol and a molecular formula of C₇H₁₀N₂O₂. It is a solid at room temperature with a melting point of 185°C. It is very soluble in water. As an acrylamide derivative, it may be metabolized and excreted, but specific ADME data is not available. The compound is stored in a dry, cool place.
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| Toxicity/Toxicokinetics |
Toxicity Data
LC (Rat) > 41 ppm/6 hours N,N'-Methylenebisacrylamide is classified for research use only and is not intended for human or veterinary applications. Standard safety precautions include handling with appropriate personal protective equipment (gloves, lab coat, safety goggles) in a well-ventilated area. The compound should be stored in a dry, cool place away from incompatible materials. Acute toxicity data is limited. As with all research chemicals, appropriate laboratory safety practices should be followed. No specific LD₅₀ values are available in the public domain. |
| Additional Infomation |
N,n'-Methylenebisacrylamide is a white crystalline powder, odorless. (NTP, 1992)
N,N'-Methylenebisacrylamide (Bisacrylamide, CAS 110-26-9) is a biochemical reagent with the molecular formula C₇H₁₀N₂O₂ and a molecular weight of 154.17 g/mol. It is widely used as a cross-linking agent in the preparation of polyacrylamide gels for electrophoresis. The compound is classified as a research-use-only reagent and is available from multiple commercial suppliers. |
| Molecular Formula |
C7H10N2O2
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| Molecular Weight |
154.17
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| Exact Mass |
154.074
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| CAS # |
110-26-9
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| Related CAS # |
26949-19-9
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| PubChem CID |
8041
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| Appearance |
White to off-white solid powder
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| Density |
1.1±0.1 g/cm3
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| Boiling Point |
445.1±41.0 °C at 760 mmHg
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| Melting Point |
365 °F (with decomposition) (NTP, 1992)
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| Flash Point |
215.0±27.8 °C
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| Vapour Pressure |
0.0±1.1 mmHg at 25°C
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| Index of Refraction |
1.475
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| LogP |
-1.44
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
2
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
11
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| Complexity |
167
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| Defined Atom Stereocenter Count |
0
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| SMILES |
O=C(C([H])=C([H])[H])N([H])C([H])([H])N([H])C(C([H])=C([H])[H])=O
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| InChi Key |
ZIUHHBKFKCYYJD-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C7H10N2O2/c1-3-6(10)8-5-9-7(11)4-2/h3-4H,1-2,5H2,(H,8,10)(H,9,11)
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| Chemical Name |
N-[(prop-2-enoylamino)methyl]prop-2-enamide
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 25 mg/mL (162.16 mM)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 6.4863 mL | 32.4317 mL | 64.8635 mL | |
| 5 mM | 1.2973 mL | 6.4863 mL | 12.9727 mL | |
| 10 mM | 0.6486 mL | 3.2432 mL | 6.4863 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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