| Size | Price | Stock | Qty |
|---|---|---|---|
| 10g |
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| Other Sizes |
| Targets |
Biochemical; tree resin
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|---|---|
| ln Vitro |
Gum guaiac, a tree resin, serves as a natural source of 2,5-di-(4-hydroxy-3-methoxyphenyl)-3,4-dimethylfuran, commonly known as α-guaiaconic acid. This phenolic compound functions as a chromogenic substrate for peroxidases. In the presence of oxidizing agents—whether organic or inorganic—α-guaiaconic acid undergoes oxidation, forming a blue-colored quinone. This property enables gum guaiac to trigger a reaction capable of detecting trace amounts of heme from hemoglobin when peroxide is present. Additionally, when prepared as a 67% w/v solution in 96% ethanol, gum guaiac is used in the Nobles test to assess the production of extracellular oxidases.
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| References |
[1]. Horseradish peroxidase: a modern view of a classic enzyme. Phytochemistry. 2004 Feb;65(3):249-59.
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| Additional Infomation |
Horseradish peroxidase (HRP) is a significant heme-containing enzyme that has been the subject of scientific inquiry for over a century. In recent years, substantial advancements have been made in understanding its three-dimensional structure, catalytic intermediates, reaction mechanisms, and the functional roles of specific amino acid residues. Techniques such as site-directed mutagenesis and directed evolution are now commonly employed to investigate HRP structure and function, enabling the development of engineered variants with practical applications in natural product synthesis, fine chemical production, medical diagnostics, and bioremediation. The combination of HRP with indole-3-acetic acid or its derivatives is currently under investigation as a potential strategy for targeted cancer therapies. While the molecular basis of HRP’s physiological roles—including its involvement in indole-3-acetic acid metabolism, cross-linking of biological polymers, and lignification—is becoming increasingly clear, the specific contributions of individual HRP isoenzymes to these processes remain poorly defined. Future progress in this area is expected to benefit from the recent identification and characterization of the complete peroxidase gene family in Arabidopsis thaliana.
[1]
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| CAS # |
9000-29-7
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|---|---|
| PubChem CID |
119026203
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| Appearance |
Typically exists as solid at room temperature
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| Melting Point |
85 °C (dec.)(lit.)
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| SMILES |
CC\\1=C(/C(=C/2\\C=CC(=O)C(=C2)OC)/O/C1=C/3\\C=CC(=O)C(=C3)OC)C
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| Synonyms |
Guaiacum Blue; Furoguaiacin Blue; QS5C7E9070; 2,5-Cyclohexadien-1-one, 4,4'-(3,4-dimethyl-2,5-furandiylidene)bis(2-methoxy-; ...; 9000-29-7
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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