| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| Other Sizes |
| Targets |
The unlabeled parent compound, 2-Aminonaphthalene (2-naphthylamine), is a known human bladder carcinogen and occupational hazard. It targets DNA, forming mutagenic adducts following metabolic activation by cytochrome P450 enzymes. The deuterated analog is used solely as an analytical internal standard and has no intrinsic biological activity or toxicological relevance.
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|---|---|
| ln Vitro |
Drug compounds have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as quantitative tracers while the drugs were being developed. Because deuteration may have an effect on a drug's pharmacokinetics and metabolic properties, it is a cause for concern [1].
In analytical assays, 2-Aminonaphthalene-d7 exhibits no in vitro biological activity. Its performance is assessed by its ability to co-elute with the analyte, provide a distinct mass shift (+7 Da), and yield consistent peak area ratios across a defined concentration range in GC-MS/MS or LC-MS/MS methods, enabling precise quantification of trace levels of 2-naphthylamine in urine or environmental samples. |
| ln Vivo |
There is no in vivo activity for the deuterated standard, as it is not administered to animals. In toxicology studies, it is used as an internal standard for the quantification of 2-naphthylamine in biological samples collected from exposed individuals or animals to monitor occupational exposure levels or to study metabolism and disposition of the carcinogen.
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| Enzyme Assay |
2-Aminonaphthalene-d7 is not used in enzyme/receptor binding assays. A typical analytical protocol involves reconstituting the compound in an organic solvent such as n-hexane or methanol, spiking it into calibration standards and samples at a fixed concentration (e.g., 20 ng/mL), and analyzing by GC-MS in negative chemical ionization mode, monitoring specific ions for the unlabeled and labeled forms.
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| Cell Assay |
This compound is not used in cell-based experiments. In cell culture studies of aromatic amine metabolism, 2-Aminonaphthalene-d7 is employed as an internal standard when quantifying levels of 2-naphthylamine in cell lysates or culture media following exposure of cells to the parent compound or its metabolites.
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| Animal Protocol |
There is no in vivo protocol for the labeled standard. In typical exposure or toxicology studies, animals or humans are exposed to unlabeled 2-naphthylamine. Biological samples (urine, blood) are then processed by adding the deuterated internal standard, extracting the analytes, and analyzing by GC-MS to quantify the concentration of the carcinogenic amine.
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| ADME/Pharmacokinetics |
PK properties are not applicable for this labeled standard, as it is not administered in vivo. For analytical use, 2-Aminonaphthalene-d7 is typically supplied as a solution in methanol at a concentration of 100 ug/mL. Stock solutions are stored at -20degC and protected from light. It is stable in solution for several months under proper storage conditions.
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| Toxicity/Toxicokinetics |
2-Aminonaphthalene-d7 is an isotopically labeled standard of a known human carcinogen. While the labeled compound itself is not intended for human exposure, it should be handled with extreme care using appropriate PPE (gloves, lab coat, eye protection) and used only within a certified fume hood due to the potential mutagenic properties of the unlabeled parent compound.
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| References | |
| Additional Infomation |
2-Aminonaphthalene-d7 is used for the determination of aromatic amines, including o-toluidine, 2,6-dimethylaniline, o-anisidine, 1-aminonaphthalene, 2-aminonaphthalene, 3-aminobiphenyl, and 4-aminobiphenyl, in mainstream cigarette smoke by GC/MS-NCI. The standard is compliant with regulatory guidelines for analytical method development and validation.
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| Molecular Formula |
C10H2D7N
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|---|---|
| Molecular Weight |
150.23
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| Exact Mass |
150.117
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| CAS # |
93951-94-1
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| PubChem CID |
46782451
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| Appearance |
White to off-white solid powder
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| Boiling Point |
306ºC(lit.)
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| Melting Point |
109-110ºC
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| LogP |
3.003
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
1
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| Rotatable Bond Count |
0
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| Heavy Atom Count |
11
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| Complexity |
133
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| Defined Atom Stereocenter Count |
0
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| SMILES |
C1=CC=C2C=C(C=CC2=C1)N
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| InChi Key |
JBIJLHTVPXGSAM-GSNKEKJESA-N
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| InChi Code |
InChI=1S/C10H9N/c11-10-6-5-8-3-1-2-4-9(8)7-10/h1-7H,11H2/i1D,2D,3D,4D,5D,6D,7D
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| Chemical Name |
1,3,4,5,6,7,8-heptadeuterionaphthalen-2-amine
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
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| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 6.6565 mL | 33.2823 mL | 66.5646 mL | |
| 5 mM | 1.3313 mL | 6.6565 mL | 13.3129 mL | |
| 10 mM | 0.6656 mL | 3.3282 mL | 6.6565 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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