| Size | Price | Stock | Qty |
|---|---|---|---|
| 10mg |
|
||
| 50mg |
|
||
| 100mg |
|
||
| Other Sizes |
| Targets |
Carbon-13C labeled multi-walled carbon nanotubes do not have a specific biological target as they are a nanomaterial and analytical tool. Their function is physicochemical—they can be used as a tracer for studying the biodistribution, clearance, and environmental fate of carbon nanotubes. In biological systems, carbon nanotubes can interact with cells and tissues, but these interactions are non-specific and depend on the physicochemical properties of the nanotubes.
|
|---|---|
| ln Vitro |
Drug compounds have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as quantitative tracers while the drugs were being developed. Because deuteration may have an effect on a drug's pharmacokinetics and metabolic properties, it is a cause for concern [1].
The labeled nanotubes themselves do not possess intrinsic pharmacological activity in vitro. Their utility is demonstrated in tracing studies where the ¹³C label allows for the detection and quantification of the nanotubes in biological and environmental samples using mass spectrometry or other analytical techniques. The nanotubes are not used as therapeutic agents but as research tools for studying the behavior of nanomaterials. |
| ln Vivo |
The labeled nanotubes do not have in vivo biological activity and are not used for therapeutic purposes. They are used in biodistribution and toxicokinetic studies to track the fate of carbon nanotubes in animals after administration. The ¹³C label enables the differentiation of the administered nanotubes from endogenous carbon sources.
|
| Enzyme Assay |
In vitro assays for carbon-13C labeled multi-walled carbon nanotubes focus on their use as a tracer rather than a receptor-binding agent. A standard protocol involves dispersing the nanotubes in an appropriate medium (e.g., water with a surfactant) and adding them to cell cultures or environmental samples. The ¹³C label is detected by isotope ratio mass spectrometry (IRMS) or other techniques to quantify the nanotubes. Quality control includes purity analysis and characterization of the nanotubes (e.g., diameter, length).
|
| Cell Assay |
In vitro cell culture experiments with the labeled nanotubes are conducted to study their cellular uptake, toxicity, and effects on cell function. Cells are cultured and exposed to the nanotubes at various concentrations. Cellular uptake and intracellular localization are assessed using microscopy and other techniques. The ¹³C label allows for quantification of the nanotubes in cell lysates.
|
| Animal Protocol |
In vivo animal studies with the labeled nanotubes are conducted to study their biodistribution, clearance, and toxicity. Animals are administered the nanotubes via various routes (e.g., intravenous, oral, inhalation), and tissues are collected at various time points. The ¹³C label allows for the quantification of the nanotubes in tissues using IRMS.
|
| ADME/Pharmacokinetics |
Pharmacokinetic properties of carbon-13C labeled multi-walled carbon nanotubes are studied using the ¹³C label as a tracer. The nanotubes are typically not absorbed systemically after oral administration but can distribute to various organs after intravenous injection. The elimination half-life can be long, and nanotubes may accumulate in the liver and spleen.
|
| Toxicity/Toxicokinetics |
Toxicological data for the labeled nanotubes are similar to those for unlabeled MWCNTs. Carbon nanotubes can cause pulmonary inflammation and fibrosis after inhalation exposure. The ¹³C label does not significantly alter the toxicity profile. Standard laboratory safety precautions should be followed when handling nanotubes.
|
| References | |
| Additional Infomation |
carbon-13 atom is a carbon atom.
Carbon-13C labeled multi-walled carbon nanotubes are used as a tracer for studying the biodistribution, environmental fate, and toxicity of carbon nanotubes. They are also known as ¹³C-MWCNTs. The compound is used in materials science, nanotechnology, and biomedical research. |
| Molecular Formula |
13C
|
|---|---|
| Molecular Weight |
13.00
|
| Exact Mass |
17.034
|
| CAS # |
14762-74-4
|
| Related CAS # |
Carbon;7440-44-0
|
| PubChem CID |
105026
|
| Appearance |
Dark gray to black solid powder
|
| Melting Point |
3550ºC(lit.)
|
| LogP |
0.636
|
| Hydrogen Bond Donor Count |
0
|
| Hydrogen Bond Acceptor Count |
0
|
| Rotatable Bond Count |
0
|
| Heavy Atom Count |
1
|
| Complexity |
0
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
CC(CCC[C@H](CCC[C@H](CCCC(CC(=O)O)C)C)C)C
|
| InChi Key |
VNWKTOKETHGBQD-OUBTZVSYSA-N
|
| InChi Code |
InChI=1S/CH4/h1H4/i1+1
|
| Chemical Name |
carbane
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 76.9231 mL | 384.6154 mL | 769.2308 mL | |
| 5 mM | 15.3846 mL | 76.9231 mL | 153.8462 mL | |
| 10 mM | 7.6923 mL | 38.4615 mL | 76.9231 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
