| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| Other Sizes |
| Targets |
MQCA-d4 does not have a specific biological target as it is an analytical standard. Its non-deuterated form, MQCA, is a metabolite of quinoxaline antibiotics that do not exert direct pharmacological effects. MQCA is used as a marker for the presence of these antibiotics in animal products. The labeled compound is used as an internal standard for the quantification of MQCA in food and biological samples.
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|---|---|
| ln Vitro |
Drug compounds have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as quantitative tracers while the drugs were being developed. Because deuteration may have an effect on a drug's pharmacokinetics and metabolic properties, it is a cause for concern [1].
The deuterated compound does not possess in vitro biological activity. MQCA is a metabolite of quinoxaline antibiotics and is not pharmacologically active. The labeled compound is used as an analytical standard for food safety testing. |
| ln Vivo |
MQCA-d4 does not have in vivo biological activity and is not used for therapeutic purposes. MQCA is a marker residue used to monitor the use of quinoxaline antibiotics in food-producing animals. The labeled compound is used as an internal standard for the quantification of MQCA in food safety testing.
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| Enzyme Assay |
In vitro assays for MQCA-d4 focus on its use as an analytical standard. A standard protocol involves preparing a solution of the compound in an appropriate solvent (e.g., methanol) and using it as an internal standard for LC-MS/MS analysis of MQCA in animal tissues, feed, or food products. The compound is added to samples before extraction and cleanup procedures. Quantification is performed by monitoring specific mass transitions for the labeled and unlabeled compounds.
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| Cell Assay |
Cell-based experiments are not performed with MQCA-d4, as it is an analytical standard.
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| Animal Protocol |
In vivo animal studies are not conducted with MQCA-d4. When used in residue analysis, the labeled compound serves as an internal standard for the quantification of MQCA in animal tissues or biological fluids.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of MQCA-d4 are not characterized, as it is not a drug substance. MQCA is a metabolite of quinoxaline antibiotics that is excreted in urine and feces. The labeled compound is used as an internal standard for food safety testing.
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| Toxicity/Toxicokinetics |
Toxicological data for MQCA-d4 are not available. Quinoxaline antibiotics have been associated with toxicity, and MQCA is monitored as a marker residue. Standard laboratory safety precautions should be followed when handling this compound.
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| References | |
| Additional Infomation |
MQCA-d4 is a stable isotope-labeled internal standard used for the quantification of MQCA in food safety testing for quinoxaline antibiotics. It is also known as 3-methyl-2-quinoxalinecarboxylic acid-d4. The compound is used in analytical method development, quality control, and regulatory compliance testing.
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| Molecular Formula |
C10H8N2O2
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|---|---|
| Molecular Weight |
192.207368850708
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| Exact Mass |
192.083
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| CAS # |
2244217-93-2
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| Related CAS # |
3-Methyl-2-quinoxalinecarboxylic acid;74003-63-7
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| PubChem CID |
139024628
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| Appearance |
Off-white to light brown solid powder
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| LogP |
1.5
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
1
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| Heavy Atom Count |
14
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| Complexity |
232
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| Defined Atom Stereocenter Count |
0
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| SMILES |
N1=C2C(C([2H])=C([2H])C([2H])=C2[2H])=NC(C)=C1C(O)=O
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| InChi Key |
BJPNADFNSANIPF-QFFDRWTDSA-N
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| InChi Code |
InChI=1S/C10H8N2O2/c1-6-9(10(13)14)12-8-5-3-2-4-7(8)11-6/h2-5H,1H3,(H,13,14)/i2D,3D,4D,5D
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| Chemical Name |
5,6,7,8-tetradeuterio-3-methylquinoxaline-2-carboxylic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.2026 mL | 26.0132 mL | 52.0264 mL | |
| 5 mM | 1.0405 mL | 5.2026 mL | 10.4053 mL | |
| 10 mM | 0.5203 mL | 2.6013 mL | 5.2026 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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