| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| Other Sizes |
| Targets |
9H-Fluoren-2-ol-d9 does not interact with a specific biological target. It is an analytical standard used solely for quantification purposes, with the deuterium label enabling mass discrimination in LC-MS/MS and GC-MS assays against the non-deuterated analyte 9H-Fluoren-2-ol.
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|---|---|
| ln Vitro |
Drug compounds have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as tracers that influence measurement during the drug development process. It's possible that the pharmacokinetics and functional range of medications contribute to the concern over mutagenesis [1].
This deuterated standard exhibits no inherent biological or pharmacological activity. Its analytical activity is defined by its performance in mass spectrometry, where it perfectly co-elutes with the target analyte and provides a distinct mass shift (+9 Da) for accurate peak integration and quantification in complex biological matrices. |
| ln Vivo |
There is no in vivo activity for this compound, as it is a stable isotope-labeled internal standard not intended for systemic administration. It is employed ex vivo as a quality control tool in method validation and bioanalysis of biological samples for environmental or biomedical research.
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| Enzyme Assay |
As a reference standard, 9H-Fluoren-2-ol-d9 is not used in enzyme/receptor binding assays. For analytical use, stock solutions are prepared in an appropriate solvent such as methanol or acetonitrile, and diluted with matched matrix to prepare a calibration curve. The compound is injected into GC-MS or LC-MS, monitoring the mass transitions of both the labeled and unlabeled analyte.
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| Cell Assay |
This compound is not used in cell-based experiments. It serves as an internal standard for the quantification of 9H-Fluoren-2-ol in complex biological fluids such as plasma, urine, or tissue homogenates. Quality control samples containing the internal standard are analyzed in parallel with study samples to ensure batch-to-batch consistency.
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| Animal Protocol |
There is no animal protocol for the labeled standard itself. In typical bioanalytical studies, animals are dosed with the unlabeled compound (e.g., 9H-Fluoren-2-ol), and plasma or tissue samples are collected. The stable isotope-labeled internal standard is then added to these samples during sample preparation prior to instrumental analysis to quantify the test compound.
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| ADME/Pharmacokinetics |
PK properties are not applicable for this labeled standard, as it is not administered in vivo. However, when used as an internal standard in PK studies of 9H-Fluoren-2-ol, it is typically formulated in organic solvents (e.g., methanol, acetonitrile, or DMSO) at ug/mL concentrations and added to biological samples during workup.
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| Toxicity/Toxicokinetics |
As a stable isotope-labeled analytical standard, 9H-Fluoren-2-ol-d9 is considered a low-toxicity chemical reagent. Standard safety precautions for handling organic solvents apply. The compound is not intended for human or animal use and is for research purposes only.
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| References | |
| Additional Infomation |
9H-Fluoren-2-ol-d9 is a deuterium-labeled analog of the aromatic compound NSC 31248. It is produced under ISO 17034 accredited quality systems, ensuring high purity and certified concentration. Stable heavy isotope-labeled compounds like this are extensively employed as tracers for quantitation during the drug development process and for method validation by regulatory agencies.
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| Molecular Formula |
C13HD9O
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|---|---|
| Molecular Weight |
191.27
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| Exact Mass |
191.13
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| CAS # |
922510-18-7
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| PubChem CID |
46781810
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| Appearance |
Typically exists as solid at room temperature
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| Density |
1.303g/cm3
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| Boiling Point |
372.079ºC at 760 mmHg
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| Flash Point |
182.013ºC
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| Index of Refraction |
1.683
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| LogP |
2.963
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
1
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| Rotatable Bond Count |
0
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| Heavy Atom Count |
14
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| Complexity |
213
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| Defined Atom Stereocenter Count |
0
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| SMILES |
c1ccc-2c(c1)Cc3c2ccc(c3)O
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| InChi Key |
ZDOIAPGLORMKTR-MFNUZUOVSA-N
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| InChi Code |
InChI=1S/C13H10O/c14-11-5-6-13-10(8-11)7-9-3-1-2-4-12(9)13/h1-6,8,14H,7H2/i1D,2D,3D,4D,5D,6D,7D2,8D
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| Chemical Name |
1,3,4,5,6,7,8,9,9-nonadeuteriofluoren-2-ol
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.2282 mL | 26.1411 mL | 52.2821 mL | |
| 5 mM | 1.0456 mL | 5.2282 mL | 10.4564 mL | |
| 10 mM | 0.5228 mL | 2.6141 mL | 5.2282 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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