| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| Other Sizes |
| Targets |
This compound does not have a specific biological target. The parent compound, TDCPP, is an industrial chemical used as a flame retardant in plastics, foams, paints, textiles, and furniture. The deuterated analog (TDCPP-d15) is an analytical reference standard designed for environmental monitoring, not for biological or pharmacological interaction studies.
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| ln Vitro |
Drug compounds have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as quantitative tracers while the drugs were being developed. Because deuteration may have an effect on a drug's pharmacokinetics and metabolic properties, it is a cause for concern [1].
There is no in vitro biological activity for this compound. Its application is strictly analytical, used to determine the presence and concentration of the flame retardant TDCPP in environmental and biological matrices. It is not used for enzymatic or cell-based assays to measure bioactivity. |
| ln Vivo |
There is no in vivo activity for this compound. TDCPP is an environmental pollutant and potential carcinogen, not a therapeutic agent. The deuterated standard is used in animal studies solely as an internal standard to measure the concentration of the flame retardant in tissues for toxicokinetic studies or environmental exposure assessments.
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| Enzyme Assay |
As an analytical standard, Tris(1,3-dichloro-2-propyl) Phosphate-d15 is not used in enzyme/receptor binding protocols. A typical GC-MS or LC-MS/MS analytical protocol involves spiking the sample (e.g., water, soil, fish tissue) with a known amount of the deuterated standard. The sample is then extracted, purified, and analyzed. The signal from the d15 standard is used to calibrate the measurement of the native TDCPP.
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| Cell Assay |
There is no standard cellular protocol for the internal standard. In a toxicological study, cells are exposed to unlabeled TDCPP to study its cytotoxic effects. The deuterated standard is then added during sample processing to quantify the amount of TDCPP that has accumulated in or been metabolized by the cells using LC-MS/MS.
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| Animal Protocol |
In an animal study for toxicological assessment, rats or fish are exposed to unlabeled TDCPP. Tissue samples (e.g., liver, fat) are then collected. The deuterated internal standard is added to the homogenized tissue before extraction. The concentration of TDCPP is then determined by LC-MS/MS, using the d15 standard to correct for any analyte loss during sample cleanup.
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| ADME/Pharmacokinetics |
As an analytical standard, TDCPP-d15 is not used for its own PK profile. For in vivo studies where it is used as a standard, it is formulated as a neat liquid or a solution in an organic solvent such as acetone or n-hexane. Typical analytical concentrations range from 10 pg/uL to 100 ng/uL.
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| Toxicity/Toxicokinetics |
The toxicity of the labeled standard is not routinely considered, as it is used at trace levels. The parent compound, TDCPP, is a potential human carcinogen (Carc. 2) and is toxic to aquatic life (Aquatic Chronic 1). Standard safety precautions for handling hazardous environmental contaminants should be used.
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| References | |
| Additional Infomation |
TDCPP-d15 is a labeled flame retardant used for environmental monitoring and regulatory compliance. The heavy isotope labeling provides a unique mass tag that distinguishes it from the natural compound, allowing for precise quantitation. It is also used in toxicological studies to understand the bioaccumulation and metabolism of OPFRs in living organisms.
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| Molecular Formula |
C9H15CL6O4P
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|---|---|
| Molecular Weight |
430.904758691788
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| Exact Mass |
444.975
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| CAS # |
1447569-77-8
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| PubChem CID |
124202862
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| Appearance |
Colorless to light yellow oil
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| LogP |
3.3
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
12
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| Heavy Atom Count |
20
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| Complexity |
243
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| Defined Atom Stereocenter Count |
0
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| SMILES |
[2H]C([2H])(C([2H])(C([2H])([2H])Cl)OP(=O)(OC([2H])(C([2H])([2H])Cl)C([2H])([2H])Cl)OC([2H])(C([2H])([2H])Cl)C([2H])([2H])Cl)Cl
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| InChi Key |
ASLWPAWFJZFCKF-BXSQCBKHSA-N
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| InChi Code |
InChI=1S/C9H15Cl6O4P/c10-1-7(2-11)17-20(16,18-8(3-12)4-13)19-9(5-14)6-15/h7-9H,1-6H2/i1D2,2D2,3D2,4D2,5D2,6D2,7D,8D,9D
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| Chemical Name |
tris(1,3-dichloro-1,1,2,3,3-pentadeuteriopropan-2-yl) phosphate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.3207 mL | 11.6036 mL | 23.2072 mL | |
| 5 mM | 0.4641 mL | 2.3207 mL | 4.6414 mL | |
| 10 mM | 0.2321 mL | 1.1604 mL | 2.3207 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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