| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| Other Sizes |
| Targets |
L-5-Hydroxytryptophan-d4 shares the same metabolic target as its non-deuterated form, L-5-HTP. L-5-HTP is the direct precursor of serotonin (5-HT), a neurotransmitter involved in mood regulation, appetite, and sleep. It is converted to serotonin by aromatic L-amino acid decarboxylase. The labeled compound is used as an internal standard for the quantification of L-5-HTP and serotonin metabolites in biological samples.
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|---|---|
| ln Vitro |
Drug compounds have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as tracers that influence measurement during the drug development process. It's possible that the pharmacokinetics and functional range of medications contribute to the concern over mutagenesis [1].
The labeled compound itself does not possess intrinsic pharmacological activity in vitro; its biological activity is identical to that of L-5-HTP. L-5-HTP is a precursor of serotonin and is used to study serotonergic signaling. The labeled compound is used as an internal standard for quantifying L-5-HTP in biological samples, not for evaluating its own pharmacological activity. |
| ln Vivo |
L-5-Hydroxytryptophan-d4 is not used as a therapeutic agent; its non-deuterated form, L-5-HTP, is a dietary supplement used as an antidepressant, appetite suppressant, and sleep aid. In vivo, L-5-HTP is absorbed after oral administration and converted to serotonin in the brain. The labeled compound is used in pharmacokinetic and metabolic studies to accurately measure L-5-HTP levels in biological samples.
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| Enzyme Assay |
In vitro assays for L-5-hydroxytryptophan-d4 focus on its use as an analytical standard. A standard protocol involves preparing a solution of the compound in an appropriate solvent (e.g., methanol) and using it as an internal standard for LC-MS analysis of L-5-HTP in biological samples (e.g., plasma, urine, brain tissue). The compound is added to samples before extraction and analysis. Quantification is performed by monitoring specific mass transitions.
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| Cell Assay |
In vitro cell culture experiments are not performed with L-5-hydroxytryptophan-d4. When studying the effects of L-5-HTP in cellular systems, the non-labeled compound is used. Cells are treated with L-5-HTP, and serotonin production is measured. The labeled compound is used as an internal standard for LC-MS analysis.
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| Animal Protocol |
In vivo animal studies are not conducted with L-5-hydroxytryptophan-d4. When used in pharmacokinetic studies, the labeled compound serves as an internal standard for the quantification of L-5-HTP in animal plasma or brain tissue samples.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of L-5-hydroxytryptophan-d4 itself are not characterized, as it is not a drug substance. L-5-HTP has a bioavailability of approximately 50-60% after oral administration, with a half-life of 2-4 hours in humans. It is metabolized to serotonin and excreted as 5-hydroxyindoleacetic acid (5-HIAA). The labeled compound is used as an internal standard for pharmacokinetic studies.
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| Toxicity/Toxicokinetics |
L-5-Hydroxytryptophan-d4 is not a therapeutic agent and has not been evaluated for toxicity in humans. L-5-HTP is generally well-tolerated, with common side effects including nausea and gastrointestinal discomfort. The labeled compound is for research use only and should be handled with standard laboratory precautions.
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| References |
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| Additional Infomation |
L-5-Hydroxytryptophan-d4 is a stable isotope-labeled internal standard used in studies of serotonin metabolism and pathway quantitation. It is also known as L-5-HTP-d4 and Oxitriptan-d4. The compound is used in analytical method development, quality control, and metabolic studies.
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| Molecular Formula |
C11H8D4N2O3
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|---|---|
| Molecular Weight |
224.25
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| Exact Mass |
224.11
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| CAS # |
1246818-91-6
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| Related CAS # |
L-5-Hydroxytryptophan;4350-09-8;L-5-Hydroxytryptophan-d3;1276197-29-5;L-5-Hydroxytryptophan-d3-1
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| PubChem CID |
71749398
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| Appearance |
Light yellow to light brown solid powder
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| LogP |
1.528
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| Hydrogen Bond Donor Count |
4
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
16
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| Complexity |
272
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| Defined Atom Stereocenter Count |
1
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| SMILES |
[2H]C1=C(C(=C(C2=C1NC(=C2C[C@@H](C(=O)O)N)[2H])[2H])O)[2H]
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| InChi Key |
LDCYZAJDBXYCGN-RUTJMMHGSA-N
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| InChi Code |
InChI=1S/C11H12N2O3/c12-9(11(15)16)3-6-5-13-10-2-1-7(14)4-8(6)10/h1-2,4-5,9,13-14H,3,12H2,(H,15,16)/t9-/m0/s1/i1D,2D,4D,5D
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| Chemical Name |
(2S)-2-amino-3-(2,4,6,7-tetradeuterio-5-hydroxy-1H-indol-3-yl)propanoic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.4593 mL | 22.2965 mL | 44.5931 mL | |
| 5 mM | 0.8919 mL | 4.4593 mL | 8.9186 mL | |
| 10 mM | 0.4459 mL | 2.2297 mL | 4.4593 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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