| Size | Price | Stock | Qty |
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| 10mg |
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| 25mg |
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| 50mg |
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| 100mg |
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| 250mg |
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| Other Sizes |
| Targets |
N-Methylacetamide-d6 does not have a biological target as it is a deuterated solvent and analytical reagent rather than a pharmacologically active compound. Its function is chemical—it serves as a solvent for NMR spectroscopy, minimizing proton interference and allowing clearer observation of other nuclei. The compound is also used in organic synthesis and materials science. The labeled compound is not evaluated for biological activity against specific targets.
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| ln Vitro |
Drug compounds have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as quantitative tracers while the drugs were being developed. Because deuteration may have an effect on a drug's pharmacokinetics and metabolic properties, it is a cause for concern [1].
The labeled compound does not possess in vitro biological activity. N-Methylacetamide is a solvent and chemical reagent that is not used in pharmacological studies. The labeled compound is used as a deuterated solvent for NMR spectroscopy and as a reagent in organic synthesis. It is not used as a test compound for biological activity in cell-based assays. |
| ln Vivo |
N-Methylacetamide-d6 does not have in vivo biological activity and is not used for therapeutic purposes. Its non-deuterated form, N-methylacetamide, is used as a solvent and chemical intermediate. The labeled compound is used as a deuterated solvent for NMR spectroscopy and as a reagent in organic synthesis. It is not administered to animals in pharmacological studies.
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| Enzyme Assay |
In vitro assays for N-methylacetamide-d6 focus on its use as a solvent and analytical reagent rather than a receptor-binding agent. A standard protocol involves using the compound as a solvent for NMR spectroscopy, where samples are dissolved in NMA-d6 and analyzed by NMR. The deuterium substitution minimizes proton interference, allowing clearer observation of other nuclei. Quality control includes purity analysis (>98%).
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| Cell Assay |
Cell-based experiments are not performed with N-methylacetamide-d6, as it is a solvent and analytical reagent. The compound is used exclusively in analytical chemistry and organic synthesis applications. No cytotoxicity or cell viability studies are conducted with this compound as it is not intended for biological applications.
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| Animal Protocol |
In vivo animal studies are not conducted with N-methylacetamide-d6, as it is a solvent and analytical reagent. The compound is not used in pharmacological or toxicological studies in animals. Its applications are limited to NMR spectroscopy and organic synthesis.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of N-methylacetamide-d6 are not characterized, as it is not a drug substance. The compound is not intended for human exposure and has not been evaluated in formal pharmacokinetic studies.
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| Toxicity/Toxicokinetics |
Toxicological data for N-methylacetamide-d6 are not available. N-Methylacetamide is a solvent that can be harmful if swallowed, inhaled, or in contact with skin. Standard laboratory safety precautions should be followed when handling this compound, including the use of gloves, safety glasses, and working in a fume hood.
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| References | |
| Additional Infomation |
N-Methylacetamide-d6 is a deuterated solvent used in NMR spectroscopy, organic synthesis, and materials science. It is also known as NMA-d6. The compound is used in analytical method development and quality control. The incorporation of deuterium minimizes proton interference in NMR spectra, allowing clearer observation of other nuclei.
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| Molecular Formula |
C3HD6NO
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|---|---|
| Molecular Weight |
79.13
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| Exact Mass |
79.09
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| CAS # |
3669-73-6
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| PubChem CID |
90472358
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| Appearance |
Colorless to light yellow liquid
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| LogP |
0.143
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
1
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| Rotatable Bond Count |
0
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| Heavy Atom Count |
5
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| Complexity |
42.2
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| Defined Atom Stereocenter Count |
0
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| SMILES |
[2H]C(NC(C([2H])([2H])[2H])=O)([2H])[2H]
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| InChi Key |
OHLUUHNLEMFGTQ-WFGJKAKNSA-N
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| InChi Code |
InChI=1S/C3H7NO/c1-3(5)4-2/h1-2H3,(H,4,5)/i1D3,2D3
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| Chemical Name |
2,2,2-trideuterio-N-(trideuteriomethyl)acetamide
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 12.6374 mL | 63.1872 mL | 126.3743 mL | |
| 5 mM | 2.5275 mL | 12.6374 mL | 25.2749 mL | |
| 10 mM | 1.2637 mL | 6.3187 mL | 12.6374 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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