| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 10mg |
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| Other Sizes |
| Targets |
L-Arginine-13C6 hydrochloride shares the same metabolic role as its non-labeled form, L-arginine. L-Arginine is a conditionally essential amino acid that is a precursor for the synthesis of nitric oxide (NO), urea, creatine, and polyamines. It is a substrate for nitric oxide synthase (NOS) and arginase. The labeled compound is used as a tracer to study arginine metabolism and flux through these pathways.
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| ln Vitro |
Drug compounds have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as quantitative tracers while the drugs were being developed. Because deuteration may have an effect on a drug's pharmacokinetics and metabolic properties, it is a cause for concern [1].
The labeled compound itself does not possess intrinsic pharmacological activity in vitro; its biological activity is identical to that of L-arginine. L-Arginine is an amino acid that is incorporated into proteins and serves as a precursor for NO and other metabolites. The labeled compound is used as a tracer for quantifying arginine metabolism, not for evaluating its own biological activity. |
| ln Vivo |
L-Arginine-13C6 hydrochloride is not used as a therapeutic agent; its non-labeled form, L-arginine, is a dietary supplement and has been studied for various clinical applications, including cardiovascular health and erectile dysfunction. In vivo, arginine is metabolized to NO and urea. The labeled compound is used in metabolic flux studies to trace the fate of arginine carbon atoms.
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| Enzyme Assay |
In vitro assays for L-arginine-13C6 hydrochloride focus on its use as a metabolic tracer. A standard protocol involves preparing a solution of the compound in an appropriate solvent and adding it to cell culture media or enzyme assays. The incorporation of ¹³C into metabolites (e.g., citrulline, ornithine) is monitored by mass spectrometry. The compound is also used as an internal standard for the quantification of arginine by LC-MS.
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| Cell Assay |
In vitro cell culture experiments with L-arginine-13C6 hydrochloride are conducted to study arginine metabolism. Cells are cultured in media containing the labeled arginine, and the incorporation of ¹³C into metabolites is analyzed by mass spectrometry. The compound is used as a tracer for metabolic studies, not as a test compound for pharmacological activity.
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| Animal Protocol |
In vivo animal studies with L-arginine-13C6 hydrochloride are conducted to study arginine metabolism and flux. Animals are administered the labeled arginine, and blood, urine, or tissue samples are collected at various time points. The ¹³C enrichment in metabolites is measured by mass spectrometry to calculate metabolic flux rates.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of L-arginine-13C6 hydrochloride are identical to those of L-arginine. Arginine is absorbed from the diet and metabolized in the liver and other tissues. The labeled compound is used as a tracer for metabolic studies.
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| Toxicity/Toxicokinetics |
L-Arginine-13C6 hydrochloride is not a therapeutic agent and has not been evaluated for toxicity in humans. L-Arginine is a naturally occurring amino acid that is generally recognized as safe at recommended dietary levels. The labeled compound is for research use only and should be handled with standard laboratory precautions.
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| References | |
| Additional Infomation |
L-Arginine-13C6 hydrochloride is a stable isotope-labeled tracer used in studies of arginine metabolism, the urea cycle, and nitric oxide synthesis. It is also known as (S)-(+)-arginine-13C6 hydrochloride. The compound is used in analytical method development, quality control, and metabolic flux studies.
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| Molecular Formula |
13C6H15CLN4O2
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|---|---|
| Molecular Weight |
216.62
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| Exact Mass |
216.108
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| CAS # |
201740-91-2
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| Related CAS # |
L-Arginine hydrochloride;1119-34-2
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| PubChem CID |
16217798
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| Appearance |
White to off-white solid powder
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| Hydrogen Bond Donor Count |
5
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
5
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| Heavy Atom Count |
13
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| Complexity |
176
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| Defined Atom Stereocenter Count |
1
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| SMILES |
[13CH2]([13CH2][13C@@H]([13C](=O)O)N)[13CH2]N=[13C](N)N.Cl
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| InChi Key |
KWTQSFXGGICVPE-BJPSCUOKSA-N
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| InChi Code |
InChI=1S/C6H14N4O2.ClH/c7-4(5(11)12)2-1-3-10-6(8)9;/h4H,1-3,7H2,(H,11,12)(H4,8,9,10);1H/t4-;/m0./s1/i1+1,2+1,3+1,4+1,5+1,6+1;
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| Chemical Name |
(2S)-2-amino-5-(diamino(113C)methylideneamino)(1,2,3,4,5-13C5)pentanoic acid;hydrochloride
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O: 250 mg/mL (1154.09 mM)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.6164 mL | 23.0819 mL | 46.1638 mL | |
| 5 mM | 0.9233 mL | 4.6164 mL | 9.2328 mL | |
| 10 mM | 0.4616 mL | 2.3082 mL | 4.6164 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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