| Size | Price | Stock | Qty |
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| 1mg |
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| Other Sizes |
| Targets |
Cholesterol sulfate-d7 sodium, as an isotopically labeled internal standard, is not designed to bind to specific biological receptors. However, the non-labeled parent compound, cholesterol sulfate, has several biological targets. Cholesterol sulfate is an endogenous component of cell membranes, where it contributes to membrane stability and rigidity. It is a substrate for steroid synthesis, serving as a precursor for various steroid hormones. Cholesterol sulfate also influences lipid metabolism and participates in cellular signaling pathways, including the hedgehog signaling pathway. Additionally, cholesterol sulfate has been shown to activate certain protein kinase C (PKC) isoforms and modulate the activity of various ion channels and receptors. The deuterated version is used solely as an analytical standard and does not engage in biological interactions.
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| ln Vitro |
Drug compounds have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as quantitative tracers while the drugs were being developed. Because deuteration may have an effect on a drug's pharmacokinetics and metabolic properties, it is a cause for concern [1].
As a deuterium-labeled internal standard, cholesterol sulfate-d7 sodium itself does not possess intrinsic in vitro biological activity. It is used exclusively for analytical quantification purposes. The non-labeled cholesterol sulfate, however, is an endogenous molecule with various biological functions. In vitro, cholesterol sulfate has been shown to stabilize cell membranes, inhibit complement activation, and regulate the activity of certain enzymes, including cholesterol sulfotransferase and steroid sulfatase. It can also influence the activity of ion channels and receptors. Cholesterol sulfate-d7 sodium serves as an internal standard for the accurate quantification of cholesterol sulfate in biological samples, such as plasma, tissues, and cell culture supernatants, using mass spectrometry-based methods. |
| ln Vivo |
Cholesterol sulfate-d7 sodium does not exhibit in vivo biological activity because it is an analytical standard. The non-labeled cholesterol sulfate, however, is an endogenous molecule with important physiological functions. In vivo, cholesterol sulfate is present in various tissues, including the brain, skin, and reproductive organs. It is involved in the regulation of lipid metabolism, cell membrane stability, and cellular signaling. Abnormal levels of cholesterol sulfate have been associated with several diseases, including Alzheimer's disease, cancer, and skin disorders such as X-linked ichthyosis. As an internal standard, the deuterated form may be administered in tracer amounts in animal studies to enable precise quantification of endogenous cholesterol sulfate levels or to study the pharmacokinetics of co-administered compounds.
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| Enzyme Assay |
A typical non-cellular protocol for using cholesterol sulfate-d7 sodium as an internal standard involves its incorporation into the sample preparation workflow for LC-MS analysis. A stock solution of the internal standard (1 mg/mL) is prepared in methanol or chloroform:methanol (1:1, v/v). For plasma samples, 50 uL of plasma is transferred to a glass tube. Then, 10 uL of the internal standard solution (diluted to a working concentration of 1 ug/mL) is added. Lipids are extracted using a modified Folch extraction method: 1 mL of chloroform:methanol (2:1, v/v) is added, and the mixture is vortexed for 1 minute. The mixture is centrifuged at 3,000 rpm for 10 minutes, and the lower organic layer is transferred to a clean tube. The organic solvent is evaporated under a stream of nitrogen at 40degC. The residue is reconstituted in 100 uL of methanol and transferred to an autosampler vial. The sample (5-10 uL) is injected onto an LC-MS/MS system operated in negative or positive ion mode for the specific transitions of cholesterol sulfate and the deuterated internal standard.
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| Cell Assay |
An established in vitro cellular protocol for using cholesterol sulfate-d7 sodium as an internal standard involves the quantification of cholesterol sulfate in cultured cells. Cells (e.g., 1×10⁶ hepatocytes or neuronal cells) are cultured in appropriate medium for 24 hours. After treatment with test compounds, cells are washed twice with ice-cold PBS and harvested by scraping. Cell pellets are resuspended in 200 uL of PBS, and 10 uL of cholesterol sulfate-d7 sodium internal standard (1 ug/mL) is added. Lipids are extracted by adding 1 mL of chloroform:methanol (2:1, v/v) and 200 uL of water. After vortexing and centrifugation, the lower organic phase is collected and dried under nitrogen. The residue is reconstituted in 100 uL of methanol and analyzed by LC-MS/MS. Cholesterol sulfate levels are normalized to total protein content or cell number. The internal standard corrects for extraction efficiency and matrix effects.
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| Animal Protocol |
Cholesterol sulfate-d7 sodium is typically used as an internal standard in animal studies for the quantification of cholesterol sulfate in plasma and tissues. In a typical protocol, male C57BL/6 mice (8-10 weeks old, 20-25 g) are used. Blood samples (50-100 uL) are collected via the tail vein into EDTA-coated tubes at various time points (0, 0.5, 1, 2, 4, 6, 8, 12, 24 hours) after oral or intravenous administration of a test compound. Plasma is separated by centrifugation (2,000 g, 10 minutes, 4degC). For tissue analysis, mice are euthanized at the final time point, and liver, brain, and other tissues are harvested, rinsed with PBS, snap-frozen in liquid nitrogen, and stored at -80degC. Tissues are homogenized in PBS (100 mg tissue/1 mL PBS) using a tissue homogenizer. For extraction, 50 uL of plasma or tissue homogenate is mixed with 10 uL of internal standard solution (1 ug/mL) and 1 mL of chloroform:methanol (2:1, v/v). After centrifugation, the organic phase is collected, dried, and reconstituted in methanol for LC-MS/MS analysis. The internal standard corrects for variations in sample preparation and matrix effects.
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| ADME/Pharmacokinetics |
As an analytical internal standard, cholesterol sulfate-d7 sodium is not characterized by typical pharmacokinetic parameters. It is not intended to be administered as a test article for PK studies. The non-labeled cholesterol sulfate is an endogenous metabolite present in biological systems, with plasma concentrations typically in the range of 0.1-1 uM in humans. Its distribution is tissue-specific, with higher concentrations in the brain and skin. The deuterium-labeled version, when used as an internal standard, is added exogenously to samples in known amounts and does not contribute to the in vivo PK profile of any test compound. Formal PK studies of the deuterated compound itself have not been conducted, as its sole purpose is to serve as a quantitative reference for mass spectrometry-based assays.
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| Toxicity/Toxicokinetics |
Toxicity data specific to cholesterol sulfate-d7 sodium are not available, as this compound is intended for use as an analytical internal standard and is not a therapeutic agent. The non-labeled cholesterol sulfate is an endogenous metabolite found in all mammalian tissues and is generally considered non-toxic at physiological concentrations. At the concentrations typically used for internal standardization (ng/mL to ug/mL range in final sample extracts), no toxic effects are expected. Standard laboratory safety precautions should be followed when handling the compound, including the use of gloves, lab coats, and safety glasses. Avoid inhalation, ingestion, and direct skin or eye contact. The compound should be stored at -20degC or 4degC in a tightly sealed container, protected from light and moisture. It is intended for research use only and should not be used in humans or animals for therapeutic or diagnostic purposes.
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| References | |
| Additional Infomation |
Cholesterol sulfate-d7 sodium (CAS# 2687961-01-7) is a stable isotope-labeled compound with a molecular weight of 495.74. The molecular formula is C27H38D7NaO4S, and the chemical name is Cholest-5-en-25,26,26,26,27,27,27-d7-3-ol, 3-(hydrogen sulfate), sodium salt (1:1), (3beta). It is also known as cholesterol sulfate-d7 sodium salt, and its isotopic purity is typically greater than 98%. The compound is supplied as a solid powder with a purity of ≥98% and should be stored at -20degC or 4degC, sealed, and protected from moisture. Cholesterol sulfate is an endogenous component of cell membranes, where it serves as a substrate for steroid synthesis, influences lipid metabolism, and stabilizes cell membranes. The deuterated version is intended for use as an internal standard for the quantification of cholesterol sulfate by GC- or LC-MS. This product is for research use only and is not approved for clinical or veterinary applications.
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| Molecular Formula |
C27H45NAO4S
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|---|---|
| Molecular Weight |
495.741710424423
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| Exact Mass |
495.337
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| CAS # |
2687961-01-7
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| PubChem CID |
162640504
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| Appearance |
White to off-white solid powder
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
7
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| Heavy Atom Count |
33
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| Complexity |
819
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| Defined Atom Stereocenter Count |
8
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| SMILES |
S(=O)(=O)([O-])O[C@H]1CC[C@@]2(C)C(C1)=CC[C@@H]1[C@@H]2CC[C@]2(C)[C@@H]([C@H](C)CCCC([2H])(C([2H])([2H])[2H])C([2H])([2H])[2H])CC[C@H]21.[Na+]
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| InChi Key |
LMPVQXVJTZWENW-CPCPJSHUSA-M
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| InChi Code |
InChI=1S/C27H46O4S.Na/c1-18(2)7-6-8-19(3)23-11-12-24-22-10-9-20-17-21(31-32(28,29)30)13-15-26(20,4)25(22)14-16-27(23,24)5;/h9,18-19,21-25H,6-8,10-17H2,1-5H3,(H,28,29,30);/q;+1/p-1/t19-,21+,22+,23-,24+,25+,26+,27-;/m1./s1/i1D3,2D3,18D;
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| Chemical Name |
sodium;[(3S,8S,9S,10R,13R,14S,17R)-10,13-dimethyl-17-[(2R)-6,7,7,7-tetradeuterio-6-(trideuteriomethyl)heptan-2-yl]-2,3,4,7,8,9,11,12,14,15,16,17-dodecahydro-1H-cyclopenta[a]phenanthren-3-yl] sulfate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.0172 mL | 10.0859 mL | 20.1719 mL | |
| 5 mM | 0.4034 mL | 2.0172 mL | 4.0344 mL | |
| 10 mM | 0.2017 mL | 1.0086 mL | 2.0172 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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