| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 50mg |
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| Targets |
The primary target of 2-deoxy-D-glucose 6-phosphate disodium is hexokinase, the first enzyme in the glycolytic pathway. The compound is an ATP-competitive, 2-deoxy-D-glucose non-competitive hexokinase inhibitor with a Ki value of 1.45 mM for bovine heart hexokinase. It also inhibits the function of glucose-6-phosphate isomerase. By inhibiting these enzymes, the compound blocks glycolysis and disrupts cellular energy metabolism.
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| ln Vitro |
In vitro studies demonstrate that 2-deoxy-D-glucose 6-phosphate disodium inhibits glycolysis in a concentration-dependent manner. The compound acts as a competitive inhibitor of hexokinase, competing with ATP for binding to the enzyme. It is produced in mammalian cells by the action of hexokinase on 2-DG, and its accumulation leads to the inhibition of glucose metabolism.
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| ln Vivo |
In vivo activity data for 2-deoxy-D-glucose 6-phosphate disodium are related to its role as a glycolysis inhibitor. The compound is used to study the effects of glycolytic inhibition on cellular metabolism, energy homeostasis, and tumor growth. By inhibiting glycolysis, it may have potential applications in cancer research where glycolytic dependence is a hallmark of many tumors.
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| Enzyme Assay |
Non-cellular assays for 2-deoxy-D-glucose 6-phosphate disodium typically involve measuring its inhibitory activity against purified hexokinase. The enzyme is incubated with the compound, ATP, and glucose, and the production of glucose-6-phosphate is measured spectrophotometrically. IC50 or Ki values are determined from dose-response curves. These cell-free systems allow for precise characterization of the compound's inhibitory potency.
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| Cell Assay |
Cellular assays for 2-deoxy-D-glucose 6-phosphate disodium are conducted using cancer cell lines to assess its effects on glycolysis and cell viability. Cells are treated with the compound, and glucose uptake, lactate production, and ATP levels are measured. Cell proliferation and viability are assessed using MTT or similar assays. The compound's ability to sensitize cells to other anticancer agents may also be evaluated.
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| Animal Protocol |
In vivo animal experiments for 2-deoxy-D-glucose 6-phosphate disodium are typically conducted in tumor xenograft models. The compound is administered orally or intravenously, and tumor growth, glucose metabolism (using PET imaging with 18F-FDG), and survival are assessed. These studies evaluate the compound's potential as an anticancer agent and its effects on tumor metabolism.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of 2-deoxy-D-glucose 6-phosphate disodium are related to its use as a research tool. The compound has a molecular weight of 288.10 g/mol and is supplied as a white to off-white solid. It is soluble in water and should be stored at -20°C. Its absorption, distribution, metabolism, and excretion depend on the route of administration and the experimental model.
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| Toxicity/Toxicokinetics |
Toxicological data for 2-deoxy-D-glucose 6-phosphate disodium are limited, as it is a research reagent not intended for human use. The compound is for research use only. Standard laboratory safety precautions should be followed when handling the compound. No significant toxicity has been reported at the concentrations used in research studies.
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| References | |
| Additional Infomation |
Other information includes 2-deoxy-D-glucose 6-phosphate disodium's role as a glucose analog that inhibits glycolysis. It inhibits the function of hexokinase and glucose-6-phosphate isomerase. The compound is produced in mammalian cells by the action of hexokinase on 2-DG and acts as a competitive inhibitor of glucose metabolism. It is used to study glycolysis and cellular energy metabolism in research settings. The compound is available as a research reagent with ≥98% purity.
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| Molecular Formula |
C6H11NA2O8P
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|---|---|
| Molecular Weight |
288.10
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| Exact Mass |
266.017
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| CAS # |
33068-19-8
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| Related CAS # |
2-Deoxy-D-glucose 6-phosphate;3573-50-0
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| PubChem CID |
91871894
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| Appearance |
White to off-white solid powder
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
8
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| Rotatable Bond Count |
6
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| Heavy Atom Count |
17
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| Complexity |
226
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| Defined Atom Stereocenter Count |
3
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| SMILES |
O=CC[C@H]([C@@H]([C@@H](COP([O-])([O-])=O)O)O)O.[Na+].[Na+]
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| InChi Key |
OKITYZJBWJMLMD-SYDFPMGNSA-L
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| InChi Code |
InChI=1S/C6H13O8P.2Na/c7-2-1-4(8)6(10)5(9)3-14-15(11,12)13;;/h2,4-6,8-10H,1,3H2,(H2,11,12,13);;/q;2*+1/p-2/t4-,5-,6+;;/m1../s1
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| Chemical Name |
disodium;[(2R,3S,4R)-2,3,4-trihydroxy-6-oxohexyl] phosphate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O: 250 mg/mL (867.75 mM)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.4710 mL | 17.3551 mL | 34.7102 mL | |
| 5 mM | 0.6942 mL | 3.4710 mL | 6.9420 mL | |
| 10 mM | 0.3471 mL | 1.7355 mL | 3.4710 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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