| Size | Price | |
|---|---|---|
| Other Sizes |
| Targets |
Its substrates are single-stranded RNA molecules. It cleaves the phosphodiester bond between a pyrimidine nucleotide and the adjacent nucleotide. The enzyme's active site contains histidine residues (His12 and His119) that are critical for its catalytic activity.
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|---|---|
| ln Vitro |
RNase A is most frequently used to remove RNA during the production of genomic or plasmid DNA. Making the first stock solution of RNase A. To make 10 mg/mL RNase A stock solution, use 10 mM sodium acetate (pH 5.2); 2. Heat for 15 minutes at 100°C; 3. Add 1/10 (v/v) of 1 M Tris-HCl (pH 7.4) after cooling to room temperature, and then adjust the pH to 7.4; 4. For up to two years, store frozen at -20°C in a stable storage environment. Note: RNase precipitate will form if the RNase A solution is cooked at a pH of zero; if it is boiled at a lower pH, protein contaminants may be the cause of any precipitation. At this time, high-speed centrifugation (13,000 rpm) can be used to remove the precipitate.
RNase A is an endoribonuclease that exhibits its highest activity with single-stranded RNA. It cleaves the sequence pG-pG-pC-pA-pG to yield pG-pG-pCp and A-pG. Its activity can be inhibited by alkylation of histidine residues in the active site and is activated by potassium and sodium salts. |
| ln Vivo |
RNase A is not a therapeutic agent; it is a laboratory reagent. Its activity is studied in vitro, not in vivo. However, its biological role in the pancreas is to digest RNA in the diet. Inhibitors of RNase A, such as the ribonuclease inhibitor protein, regulate its activity within cells.
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| Enzyme Assay |
A typical RNase A activity assay involves incubating the enzyme with a defined RNA substrate in a suitable buffer (e.g., 0.2 M sodium phosphate, pH 6.4). The reaction is stopped, and the remaining intact RNA is quantified by precipitation or by measuring the increase in absorbance at 260 nm, which indicates the release of nucleotides.
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| Cell Assay |
While not typically used in cell culture as an active biological modulator, RNase A can be added to cell lysates to remove RNA during protein or DNA extraction. Its activity in this context is verified by the absence of RNA contamination in the final sample, as confirmed by agarose gel electrophoresis.
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| Animal Protocol |
Animal studies are not conducted with RNase A as a therapeutic or investigative agent. It is a reagent used in the laboratory to process samples from animals. For example, it is used to remove RNA from plasmid DNA preparations isolated from bacteria.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties are not relevant for RNase A, as it is a research reagent. Its physical properties are well-characterized: molecular mass 13.7 kDa, extinction coefficient E1% = 7.1 at 280 nm, isoelectric point pI = 9.6, optimal temperature 60°C, and optimal pH 7.6.
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| Toxicity/Toxicokinetics |
Toxicological data are not typically provided, as RNase A is a common laboratory reagent. It is considered safe to handle with standard laboratory precautions. It is not intended for diagnostic or therapeutic use. Its safety data sheet (SDS) should be consulted for specific safety information.
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| References |
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| Additional Infomation |
A dipeptide neuropeptide naturally found in muscles.
See also: Papain (note moved to). RNase A is one of the most widely used enzymes in molecular biology research. Its primary applications include the removal of RNA from DNA plasmid preparations and protein samples, RNase protection assays, and RNA sequence analysis. Its high stability and specific activity make it an essential tool for nucleic acid research. |
| Molecular Formula |
C9H14N4O3
|
|---|---|
| Molecular Weight |
226.2325
|
| Exact Mass |
226.106
|
| CAS # |
9001-99-4
|
| PubChem CID |
9369
|
| Appearance |
White to off-white solid powder
|
| Density |
1.4±0.1 g/cm3
|
| Boiling Point |
656.2±55.0 °C at 760 mmHg
|
| Melting Point |
243 ºC (dec.)
|
| Flash Point |
350.7±31.5 °C
|
| Vapour Pressure |
0.0±2.1 mmHg at 25°C
|
| Index of Refraction |
1.591
|
| LogP |
-2.17
|
| Hydrogen Bond Donor Count |
4
|
| Hydrogen Bond Acceptor Count |
5
|
| Rotatable Bond Count |
6
|
| Heavy Atom Count |
16
|
| Complexity |
259
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
C1=C(NC=N1)CC(C(=O)O)NC(=O)CCN
|
| InChi Key |
CQOVPNPJLQNMDC-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C9H14N4O3/c10-2-1-8(14)13-7(9(15)16)3-6-4-11-5-12-6/h4-5,7H,1-3,10H2,(H,11,12)(H,13,14)(H,15,16)
|
| Chemical Name |
2-(3-aminopropanoylamino)-3-(1H-imidazol-5-yl)propanoic acid
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
H2O: 100 mg/mL
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.4203 mL | 22.1014 mL | 44.2028 mL | |
| 5 mM | 0.8841 mL | 4.4203 mL | 8.8406 mL | |
| 10 mM | 0.4420 mL | 2.2101 mL | 4.4203 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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