| Size | Price | Stock | Qty |
|---|---|---|---|
| 10g |
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| 25g |
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| Other Sizes |
| Targets |
Sephadex LH-20 does not have a specific biological "target". It is a chromatographic medium, not a biologically active compound. Its function is based on its physicochemical properties, specifically its porous structure and its ability to interact with molecules through size-exclusion and adsorption mechanisms. It separates molecules based on their molecular weight and their affinity for the gel matrix.
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|---|---|
| ln Vitro |
In vitro, Sephadex LH-20 is used as a tool for the separation and purification of compounds. Its "activity" is its performance as a chromatographic medium, which is assessed by its ability to resolve a mixture of compounds into its individual components. This is measured by the resolution, peak shape, and recovery of the separated compounds.
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| ln Vivo |
In vivo, Sephadex LH-20 is not used as a therapeutic agent. Its application is entirely in vitro as a laboratory reagent.
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| Enzyme Assay |
A typical protocol involves packing Sephadex LH-20 into a glass column and equilibrating it with an appropriate solvent system. The sample is then applied to the column, and fractions are collected as the solvent flows through. The separated compounds are detected by UV absorbance, refractive index, or other methods.
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| Cell Assay |
Sephadex LH-20 is not used in cell culture. It is a chromatographic medium for separating molecules in solution.
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| Animal Protocol |
Sephadex LH-20 is not used in animal studies as an active compound. However, it can be used to purify compounds from animal tissues for subsequent analysis.
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| ADME/Pharmacokinetics |
Sephadex LH-20 is a cross-linked dextran gel. Its properties, such as bead size, pore size, and exclusion limit, are characterized by the manufacturer. It is typically stored at room temperature, protected from moisture.
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| Toxicity/Toxicokinetics |
Sephadex LH-20 is considered to be non-toxic and is a standard laboratory reagent. Standard laboratory safety precautions should be followed when handling the gel and the solvents used with it.
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| References |
[1]. Cyril Auger, et al. The red wine extract-induced activation of endothelial nitric oxide synthase is mediated by a great variety of polyphenolic compounds. Mol Nutr Food Res. 2010 Jul;54 Suppl 2:S171-83.
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| Additional Infomation |
Sephadex LH-20 is a widely used and versatile size-exclusion chromatography medium for the separation of natural products and small molecules. Its ability to swell in organic solvents makes it particularly valuable for separating lipophilic compounds. It is an essential tool in natural product chemistry, pharmacognosy, and biochemistry for the purification and analysis of complex mixtures. It is not a drug.
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| Molecular Formula |
C9H8N2O3S
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|---|---|
| Molecular Weight |
224.2364
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| Exact Mass |
224.025
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| CAS # |
9041-37-6
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| PubChem CID |
57637373
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| Appearance |
White to off-white solid powder
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| LogP |
1.4
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
15
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| Complexity |
297
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| Defined Atom Stereocenter Count |
0
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| SMILES |
CC(=O)N1C2=C(C=N1)SC(=C2)C(=O)OC
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| InChi Key |
XELZGAJCZANUQH-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C9H8N2O3S/c1-5(12)11-6-3-7(9(13)14-2)15-8(6)4-10-11/h3-4H,1-2H3
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| Chemical Name |
methyl 1-acetylthieno[3,2-c]pyrazole-5-carboxylate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O: < 0.1 mg/mL
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.4595 mL | 22.2975 mL | 44.5951 mL | |
| 5 mM | 0.8919 mL | 4.4595 mL | 8.9190 mL | |
| 10 mM | 0.4460 mL | 2.2298 mL | 4.4595 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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