| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| Other Sizes |
| Targets |
Glyphosate-13C2,15N targets the same biological target as glyphosate, which is the shikimate pathway in plants. Specifically, glyphosate inhibits the enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), which is essential for the synthesis of aromatic amino acids in plants. This pathway is not found in animals, making glyphosate selectively toxic to plants.
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| ln Vitro |
Drug compounds have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as tracers that influence measurement during the drug development process. It's possible that the pharmacokinetics and functional range of medications contribute to the concern over mutagenesis [2].
Glyphosate-13C2,15N is an isotopically labeled form of the herbicide glyphosate. As a labeled analog, it exhibits the same herbicidal activity as unlabeled glyphosate. Its in vitro activity can be assessed by measuring the inhibition of EPSPS enzyme activity in plant extracts. |
| ln Vivo |
Glyphosate-13C2,15N exhibits the same in vivo herbicidal activity as glyphosate. It is absorbed by plants and translocated to growing points, where it inhibits the shikimate pathway, leading to the depletion of aromatic amino acids and plant death. As a labeled compound, it is primarily used to study the absorption, translocation, and degradation pathways of glyphosate in plants and soil.
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| Enzyme Assay |
The inhibition of EPSPS by Glyphosate-13C2,15N can be assessed using an in vitro enzyme assay. Plant EPSPS enzyme is incubated with its substrate, phosphoenolpyruvate (PEP), and shikimate-3-phosphate (S3P) in the presence of varying concentrations of the compound. The production of EPSP is measured, and the IC50 is determined.
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| Cell Assay |
Glyphosate-13C2,15N is used in plant cell culture to study the effects of glyphosate on plant metabolism. Plant cells are treated with the labeled compound, and the incorporation of 13C and 15N into metabolic products is analyzed by mass spectrometry to study the shikimate pathway and glyphosate's mode of action.
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| Animal Protocol |
Glyphosate-13C2,15N is used in plant and soil studies to track the absorption, translocation, and degradation of glyphosate. Plants are treated with the labeled herbicide, and the distribution of 13C and 15N in plant tissues and soil is analyzed by mass spectrometry to study the fate of glyphosate in the environment.
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| ADME/Pharmacokinetics |
Glyphosate-13C2,15N has a molecular weight of 172.05 g/mol and a molecular formula of (13C)C2H8(15N)O5P. The compound has a minimum purity of 98.00% and a minimum isotopic enrichment of 99% 13C and 98% 15N. It is classified as a hazardous substance and may require additional shipping fees.
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| Toxicity/Toxicokinetics |
Toxicological data for Glyphosate-13C2,15N are not extensively detailed, as it is a research compound. Glyphosate itself is classified as a hazardous substance. The compound is not intended for human or veterinary use. Standard laboratory safety precautions, including the use of personal protective equipment (PPE), should be followed when handling the compound.
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| References |
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| Additional Infomation |
Glyphosate-13C2,15N is a research-grade compound used for studying the absorption, translocation, and degradation pathways of glyphosate in plants and soil. Its 13C and 15N labeling enables the precise tracking of glyphosate in environmental and agricultural research. The compound is also used for analytical method development and quality control applications. It is not approved for therapeutic use and is strictly for laboratory research.
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| Molecular Formula |
C13C2H815NO5P
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|---|---|
| Molecular Weight |
172.05
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| Exact Mass |
172.017
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| CAS # |
1185107-63-4
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| Related CAS # |
Glyphosate;1071-83-6
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| PubChem CID |
46780452
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| Appearance |
White to off-white solid powder
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| Density |
1.7±0.1 g/cm3
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| Melting Point |
>270°C (dec.) (lit.)
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| Index of Refraction |
1.529
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| LogP |
-4.6
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| Hydrogen Bond Donor Count |
4
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| Hydrogen Bond Acceptor Count |
6
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
10
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| Complexity |
162
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| Defined Atom Stereocenter Count |
0
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| SMILES |
C([15NH][13CH2][13C](=O)O)P(=O)(O)O
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| InChi Key |
XDDAORKBJWWYJS-QZJDPFNNSA-N
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| InChi Code |
InChI=1S/C3H8NO5P/c5-3(6)1-4-2-10(7,8)9/h4H,1-2H2,(H,5,6)(H2,7,8,9)/i1+1,3+1,4+1
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| Chemical Name |
2-(phosphonomethyl(15N)amino)acetic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.8123 mL | 29.0613 mL | 58.1226 mL | |
| 5 mM | 1.1625 mL | 5.8123 mL | 11.6245 mL | |
| 10 mM | 0.5812 mL | 2.9061 mL | 5.8123 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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