| Size | Price | Stock | Qty |
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| 50mg |
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| 100mg |
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| Other Sizes |
| Targets |
Acetamide-15N does not have a specific biological target. It is a 15N-labeled compound used as a tracer for studying nitrogen metabolism, protein synthesis, and enzymatic reactions. Its primary function is as a stable isotopic marker for studying reaction mechanisms and metabolic pathways.
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|---|---|
| ln Vitro |
Drug compounds have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as quantitative tracers while the drugs were being developed. Because deuteration may have an effect on a drug's pharmacokinetics and metabolic properties, it is a cause for concern [1].
The compound is not evaluated for traditional in vitro biological activity. Its "activity" is as a stable isotope-labeled tracer for studying nitrogen metabolism and enzymatic reactions. It is used as a stable isotopic marker to study reaction mechanisms, intermediate species formation, and product distribution. |
| ln Vivo |
No in vivo activity is applicable for Acetamide-15N, as it is not a therapeutic agent. Its use is confined to the laboratory for biochemical and pharmaceutical research. The compound is not administered to living organisms for any biological effect.
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| Enzyme Assay |
The compound is used as a stable isotopic marker to study reaction mechanisms, intermediate species formation, and product distribution. Its performance is assessed by its isotopic purity and its ability to serve as a reliable tracer in nitrogen metabolism studies.
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| Cell Assay |
Acetamide-15N is not used in standard cell culture for pharmacological studies. It is a chemical reagent used in biochemical and pharmaceutical research for studying nitrogen metabolism and enzymatic reactions. Its isotopic labeling allows for the tracking of nitrogen atoms in biological systems.
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| Animal Protocol |
Animal studies are not conducted with Acetamide-15N as an active compound. It is used as a tracer in studies of nitrogen metabolism and protein synthesis in animals. The compound is never administered to animals for therapeutic or investigative purposes.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties are not relevant for Acetamide-15N, as it is not a therapeutic agent. Its chemical properties include a molecular weight of 60.06 g/mol and a molecular formula of C2H5NO (with 15N).
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| Toxicity/Toxicokinetics |
Toxicological data for Acetamide-15N are not extensively detailed, as it is a research compound. However, acetamide is carcinogenic. The compound is not intended for human or veterinary use. Standard laboratory safety precautions, including the use of personal protective equipment (PPE), should be followed when handling the compound.
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| References | |
| Additional Infomation |
Acetamide-15N is a research-grade compound used as a stable isotopic marker for studying nitrogen metabolism, protein synthesis, and enzymatic reactions. Its 15N labeling enables the tracking of nitrogen atoms in biological and chemical systems. The compound is not approved for therapeutic use and is strictly for laboratory research.
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| Molecular Formula |
C2H515NO
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|---|---|
| Molecular Weight |
60.06
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| Exact Mass |
60.034
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| CAS # |
1449-72-5
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| Related CAS # |
Acetamide;60-35-5
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| PubChem CID |
12228446
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| Appearance |
White to off-white solid powder
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| Density |
0.947g/cm3
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| Boiling Point |
221ºC(lit.)
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| Melting Point |
78-80ºC(lit.)
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| Index of Refraction |
1.392
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| LogP |
0.191
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
1
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| Rotatable Bond Count |
0
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| Heavy Atom Count |
4
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| Complexity |
33
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| Defined Atom Stereocenter Count |
0
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| SMILES |
CC(=O)[15NH2]
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| InChi Key |
DLFVBJFMPXGRIB-LBPDFUHNSA-N
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| InChi Code |
InChI=1S/C2H5NO/c1-2(3)4/h1H3,(H2,3,4)/i3+1
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| Chemical Name |
acetamide
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: (1). This product requires protection from light (avoid light exposure) during transportation and storage. (2). Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 16.6500 mL | 83.2501 mL | 166.5002 mL | |
| 5 mM | 3.3300 mL | 16.6500 mL | 33.3000 mL | |
| 10 mM | 1.6650 mL | 8.3250 mL | 16.6500 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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