| Size | Price | Stock | Qty |
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| 5mg |
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| Other Sizes |
| Targets |
(rac)-Metanephrine-d3 hydrochloride is a labeled form of metanephrine, which is a metabolite of epinephrine and norepinephrine. As a stable isotope-labeled compound, it does not have a specific biological target for its use as an analytical standard. Its primary function is as an internal standard for the quantification of metanephrine in diagnostic and research applications. The compound is not intended to interact with biological targets.
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| ln Vitro |
Drug compounds have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as quantitative tracers while the drugs were being developed. Because deuteration may have an effect on a drug's pharmacokinetics and metabolic properties, it is a cause for concern [1].
(rac)-Metanephrine-d3 hydrochloride itself is not evaluated for traditional in vitro biological activity. Its "activity" is as an analytical tool for quantitative analysis by LC-MS/MS. It is used as an internal standard to accurately measure metanephrine levels in biological samples. The compound's isotopic labeling allows for precise quantification in complex matrices. |
| ln Vivo |
(rac)-Metanephrine-d3 hydrochloride is not administered as a therapeutic agent in vivo. Its primary application is as an analytical standard for the quantification of metanephrine in diagnostic tests. The compound is used in laboratory settings to measure the concentration of metanephrine in biological samples. It is not used in animal models as an active treatment.
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| Enzyme Assay |
(rac)-Metanephrine-d3 hydrochloride is used as an internal standard in analytical chemistry. Its performance is assessed by its ability to provide accurate and precise quantification of metanephrine in complex matrices. The compound is typically analyzed by LC-MS/MS. Its isotopic purity and chemical purity are critical parameters for its use as an internal standard.
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| Cell Assay |
(rac)-Metanephrine-d3 hydrochloride is used as an internal standard in the analysis of biological samples. It is added to samples containing metanephrine to correct for variability in sample preparation and instrument performance. The labeled and unlabeled compounds are co-eluted and detected by mass spectrometry, allowing for accurate quantification of metanephrine.
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| Animal Protocol |
(rac)-Metanephrine-d3 hydrochloride is used in clinical and pharmaceutical research as an internal standard for the quantification of metanephrine in biological samples. It is added to samples from patients or animals to accurately measure metanephrine concentrations for diagnostic and research purposes. The compound itself is not administered to animals or patients.
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| ADME/Pharmacokinetics |
(rac)-Metanephrine-d3 hydrochloride has a molecular weight of 236.71 g/mol and a molecular formula of C10H13D3ClNO3. It is a deuterium-labeled form of (rac)-metanephrine hydrochloride. The compound is typically stored at -20°C for long-term stability. It is soluble in DMSO and other organic solvents. Its stability and solubility are well-characterized for use as an analytical standard.
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| Toxicity/Toxicokinetics |
Toxicological data for (rac)-Metanephrine-d3 hydrochloride are not extensively detailed, as it is a research compound. It is not intended for human or veterinary use except in clinical diagnostic applications. Standard laboratory safety precautions, including the use of personal protective equipment (PPE), should be followed when handling the compound.
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| References | |
| Additional Infomation |
(rac)-Metanephrine-d3 hydrochloride is a deuterium-labeled form of (rac)-metanephrine hydrochloride, a metabolite of epinephrine and norepinephrine. It is widely used in medical, environmental, and industrial research. The use of this compound enhances the accuracy of diagnostic tests, aiding in the effective management and treatment of related disorders. It is a biochemical reagent for life science research and is not approved for therapeutic use.
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| Molecular Formula |
C10H13D3CLNO3
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|---|---|
| Molecular Weight |
236.71
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| Exact Mass |
233.081
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| CAS # |
1215507-88-2
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| PubChem CID |
46782135
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| Appearance |
White to off-white solid powder
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| Hydrogen Bond Donor Count |
4
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
15
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| Complexity |
165
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| Defined Atom Stereocenter Count |
0
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| SMILES |
[2H]C([2H])([2H])NCC(C1=CC(=C(C=C1)O)OC)O.Cl
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| InChi Key |
HRIQFVCFOPJYEQ-NIIDSAIPSA-N
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| InChi Code |
InChI=1S/C10H15NO3.ClH/c1-11-6-9(13)7-3-4-8(12)10(5-7)14-2;/h3-5,9,11-13H,6H2,1-2H3;1H/i1D3;
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| Chemical Name |
4-[1-hydroxy-2-(trideuteriomethylamino)ethyl]-2-methoxyphenol;hydrochloride
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.2246 mL | 21.1229 mL | 42.2458 mL | |
| 5 mM | 0.8449 mL | 4.2246 mL | 8.4492 mL | |
| 10 mM | 0.4225 mL | 2.1123 mL | 4.2246 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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