| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| Other Sizes |
| Targets |
Homocapsaicin II targets cyclooxygenase-1 (COX-1) and cyclooxygenase-2 (COX-2) enzymes. By inhibiting these enzymes, it reduces the synthesis of prostaglandins, which are mediators of inflammation and pain. This mechanism is similar to that of non-steroidal anti-inflammatory drugs (NSAIDs). The compound's ability to inhibit COX enzymes makes it a subject of interest for anti-inflammatory research.
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| ln Vitro |
In vitro, Homocapsaicin II has been shown to inhibit COX-1 and COX-2 activity. Its inhibitory activity can be assessed by measuring the production of prostaglandins in cell-based or cell-free enzyme assays. The compound's IC50 values for COX-1 and COX-2 inhibition can be determined. Its anti-inflammatory activity is a key area of research.
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| ln Vivo |
Homocapsaicin II is useful for its anti-inflammatory activities. As a COX-1 and COX-2 inhibitor, it may exhibit in vivo anti-inflammatory effects similar to those of other NSAIDs. Its potential therapeutic applications include the treatment of inflammatory conditions. However, specific in vivo study details are not extensively documented in general references.
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| Enzyme Assay |
The inhibitory activity of Homocapsaicin II on COX-1 and COX-2 can be assessed using an in vitro enzyme assay. Purified COX enzymes are incubated with arachidonic acid and varying concentrations of the compound. The production of prostaglandins is measured, and the IC50 values are determined. The compound's selectivity for COX-1 versus COX-2 can also be evaluated.
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| Cell Assay |
Homocapsaicin II is used in cell-based assays to evaluate its anti-inflammatory activity. Cells such as macrophages are treated with the compound and stimulated with inflammatory agents. The production of pro-inflammatory mediators, including prostaglandins and cytokines, is measured. The compound's ability to reduce inflammation in cell culture models can be assessed.
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| Animal Protocol |
The in vivo efficacy of Homocapsaicin II can be studied in animal models of inflammation. The compound is administered to animals, and its effects on inflammatory markers and tissue damage are assessed. However, specific animal study protocols are not detailed in general references. The compound is primarily used in research settings to study its anti-inflammatory properties.
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| ADME/Pharmacokinetics |
Homocapsaicin II has a molecular weight of 319.44 g/mol and a molecular formula of C19H29NO3. It is a lipophilic, colorless, odorless crystalline to waxy compound. The compound is typically stored at room temperature and is soluble in organic solvents. As a capsaicinoid, its pharmacokinetic properties would be characteristic of this class of compounds.
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| Toxicity/Toxicokinetics |
Toxicological data for Homocapsaicin II are not extensively detailed, as it is a research compound. It is not intended for human or veterinary use. Standard laboratory safety precautions, including the use of personal protective equipment (PPE), should be followed when handling the compound. Its safety profile would be established through dedicated toxicology studies if it were to be developed further.
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| References | |
| Additional Infomation |
Homocapsaicin II has been reported in chili peppers and data are available.
Homocapsaicin II is a pungent compound isolated from the fruits of Capsicum annuum L. It is a capsaicinoid that accounts for about 1% of the total capsaicinoids mixture in chili peppers and has approximately half the pungency of capsaicin. The compound displays significant potential to act as an effective inhibitor of COX-1 and COX-2 and is useful for its anti-inflammatory activities. It is a research-grade compound not approved for clinical use. |
| Molecular Formula |
C19H29NO3
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|---|---|
| Molecular Weight |
319.44
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| Exact Mass |
319.215
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| CAS # |
71240-51-2
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| PubChem CID |
11674147
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| Appearance |
Typically exists as solid at room temperature
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| LogP |
4.57
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
10
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| Heavy Atom Count |
23
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| Complexity |
357
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| Defined Atom Stereocenter Count |
0
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| SMILES |
OC1C=CC(CNC(=O)CCCC/C=C/C(C)CC)=CC=1OC
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| InChi Key |
MLJGZARGNROKAC-VQHVLOKHSA-N
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| InChi Code |
InChI=1S/C19H29NO3/c1-4-15(2)9-7-5-6-8-10-19(22)20-14-16-11-12-17(21)18(13-16)23-3/h7,9,11-13,15,21H,4-6,8,10,14H2,1-3H3,(H,20,22)/b9-7+
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| Chemical Name |
(E)-N-[(4-hydroxy-3-methoxyphenyl)methyl]-8-methyldec-6-enamide
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| Synonyms |
Homocapsaicin II
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.1305 mL | 15.6524 mL | 31.3048 mL | |
| 5 mM | 0.6261 mL | 3.1305 mL | 6.2610 mL | |
| 10 mM | 0.3130 mL | 1.5652 mL | 3.1305 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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