| Size | Price | Stock | Qty |
|---|---|---|---|
| 100mg |
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| Other Sizes |
| Targets |
Coumarin 343 targets monocarboxylic acid transporter protein 4 (MCT4). MCT4 is a transmembrane protein that facilitates the transport of monocarboxylates such as lactate and pyruvate across cell membranes, playing a critical role in cellular metabolism. By inhibiting MCT4, Coumarin 343 can affect the metabolic pathways of cancer cells, leading to anticancer activity. The compound also exhibits antibacterial activity, suggesting additional targets in bacterial cells. Its fluorescent properties allow it to be used as a probe for studying micro water pools and cellular processes. The compound's anticancer activity may be mediated through direct cytotoxic effects on cancer cells, while its antibacterial activity makes it useful in the study of infectious diseases.
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| ln Vitro |
In vitro studies have demonstrated that Coumarin 343 inhibits MCT4 with IC50 values ranging from 0.01-100 µM. The compound exhibits anticancer activity in cancer cells by affecting their metabolic pathways or through direct cytotoxic effects. Its antibacterial activity has been demonstrated against various bacterial strains. As a fluorescent probe, Coumarin 343 is used to visualize cellular processes with high sensitivity and specificity. The compound's hydrophilic nature makes it suitable for studying micro water pools in biological systems. Its fluorescence properties have been characterized using spectrofluorometry, with emission observed from 425 nm to 550 nm. These in vitro findings support its applications in cancer research, infectious disease studies, and biological imaging.
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| ln Vivo |
In vivo studies of Coumarin 343 are primarily focused on its applications as a fluorescent probe and its potential in photodynamic therapy. The compound's fluorescence properties allow for in vivo imaging of biological processes. Its ability to inhibit MCT4 suggests potential therapeutic applications in cancer and metabolic diseases. The compound's anticancer activity has been evaluated in animal models of cancer. Its antibacterial activity may be applicable in the study of infectious diseases in vivo. As a hydrophilic fluorescent probe, Coumarin 343 can be used to monitor micro water pools in living organisms. Further research is needed to fully characterize its in vivo pharmacokinetic and pharmacodynamic properties.
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| Enzyme Assay |
In vitro enzyme/receptor binding assays for Coumarin 343 involve testing its inhibitory activity against MCT4. MCT4 inhibition is measured using transport assays in which the uptake of radiolabeled or fluorescent substrates (such as lactate or pyruvate) is monitored in cells or membrane vesicles expressing the transporter. IC50 values are determined from dose-response curves generated across a range of compound concentrations (0.01-100 µM). For fluorescence characterization, the compound's excitation and emission spectra are recorded using a spectrofluorometer. The compound's antibacterial activity is assessed using standard antimicrobial susceptibility testing methods such as broth microdilution or agar diffusion assays to determine minimum inhibitory concentrations (MICs). All assays are performed with appropriate controls and standardized protocols to ensure reproducibility of results.
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| Cell Assay |
In vitro cell-based assays for Coumarin 343 involve culturing cancer cell lines to evaluate its anticancer activity. Cells are treated with varying concentrations of the compound (typically in the 0.01-100 µM range) for specified durations (24-72 hours). Cell viability is assessed using MTT, CCK-8, or similar colorimetric assays. Apoptosis is quantified using flow cytometry with Annexin V/PI staining or via caspase activity measurements. For MCT4 inhibition studies, lactate efflux or uptake is measured in cells treated with the compound. For antibacterial activity assessment, bacterial cultures are treated with the compound and cell viability is monitored by optical density measurements or colony counting. The compound's fluorescence properties allow for live-cell imaging studies. All experiments are performed in triplicate with appropriate controls to ensure statistical reliability.
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| Animal Protocol |
In vivo animal experiments for Coumarin 343 are conducted to evaluate its potential as a fluorescent probe and its therapeutic applications. For imaging studies, animals (typically mice or rats) are administered the compound and fluorescence is monitored using in vivo imaging systems. For anticancer studies, tumor-bearing animals are treated with the compound and tumor growth is monitored. For antibacterial studies, infected animals are treated with the compound and bacterial load is assessed. Parameters assessed include body weight, tumor size, survival, and general health. At study termination, tissues are collected for histopathological examination and fluorescence microscopy. Control groups receiving vehicle alone are included for comparison. All procedures must comply with institutional animal care and use committee guidelines.
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| ADME/Pharmacokinetics |
The pharmacokinetic properties of Coumarin 343 reflect its nature as a hydrophilic fluorescent probe. The compound has a molecular weight of 369.41 and is hydrophilic in nature, which may limit its ability to cross biological membranes. Its fluorescence properties allow for detection in biological samples using spectrofluorometry, with emission observed from 425 nm to 550 nm. The compound is used as a probe for micro water pools, indicating its utility in studying aqueous environments in biological systems. Its storage recommendations include protection from light. Complete pharmacokinetic profiling including absorption, distribution, metabolism, and excretion would require further systematic studies using appropriate analytical methods such as high-performance liquid chromatography with fluorescence detection.
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| Toxicity/Toxicokinetics |
The toxicity profile of Coumarin 343 has been evaluated in the context of its use as a fluorescent probe and research chemical. The compound is classified as a research chemical intended for laboratory use only. Its toxicity has been assessed in cell-based assays, where it has shown anticancer activity in cancer cells. The compound's antibacterial activity suggests potential effects on microbial cells. At research use concentrations, it is generally considered safe for laboratory applications with proper handling procedures. However, as with all fluorescent dyes, appropriate precautions including use of personal protective equipment and proper waste disposal are recommended. The compound is not approved for human therapeutic use and is intended for research purposes only. Long-term toxicity studies would be needed to fully establish its safety profile.
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| References | |
| Additional Infomation |
Coumarin 343 is a member of the 7-aminocoumarin family and is a fluorescent dye.
Coumarin 343 (C343, CAS# 55804-65-4) is also known as coumarin 343 and coumarine 343. It has a purity of 95.74% for research grade material. The compound is a hydrophilic fluorescent probe for the micro water pool. It inhibits monocarboxylic acid transporter protein 4 with IC50 values in the range of 0.01-100 µM. The compound exhibits anticancer and antibacterial activities. It is a coumarin dye with emission at approximately 425 nm and emission ranging from 425 nm to 550 nm. The compound is widely utilized as a fluorescent dye in biological imaging and has applications in photodynamic therapy research. It is intended for research use only. |
| Molecular Formula |
C16H15NO4
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|---|---|
| Molecular Weight |
285.29
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| Exact Mass |
285.1
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| CAS # |
55804-65-4
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| PubChem CID |
108770
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| Appearance |
Yellow to brown solid powder
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| Density |
1.5±0.1 g/cm3
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| Boiling Point |
574.8±50.0 °C at 760 mmHg
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| Melting Point |
240ºC
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| Flash Point |
301.4±30.1 °C
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| Vapour Pressure |
0.0±1.7 mmHg at 25°C
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| Index of Refraction |
1.691
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| LogP |
3.2
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
1
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| Heavy Atom Count |
21
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| Complexity |
514
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| Defined Atom Stereocenter Count |
0
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| SMILES |
C1CC2=C3C(=C4C(=C2)C=C(C(=O)O4)C(=O)O)CCCN3C1
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| InChi Key |
KCDCNGXPPGQERR-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C16H15NO4/c18-15(19)12-8-10-7-9-3-1-5-17-6-2-4-11(13(9)17)14(10)21-16(12)20/h7-8H,1-6H2,(H,18,19)
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| Chemical Name |
4-oxo-3-oxa-13-azatetracyclo[7.7.1.02,7.013,17]heptadeca-1,5,7,9(17)-tetraene-5-carboxylic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.5052 mL | 17.5260 mL | 35.0521 mL | |
| 5 mM | 0.7010 mL | 3.5052 mL | 7.0104 mL | |
| 10 mM | 0.3505 mL | 1.7526 mL | 3.5052 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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