| Size | Price | Stock | Qty |
|---|---|---|---|
| 500mg |
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| Other Sizes |
| Targets |
2-Chloro-1,4-naphthoquinone targets various biological pathways through its quinone moiety. Naphthoquinones are known to generate reactive oxygen species through redox cycling, leading to oxidative stress and cell death. The compound may target cellular thiols and proteins through Michael addition reactions. Its antimicrobial and anticancer activities are thought to be mediated through oxidative stress and interaction with cellular targets. The chlorine substituent may influence its redox properties and biological activity. Further research is needed to identify its specific molecular targets.
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| ln Vitro |
In vitro studies have demonstrated that 2-Chloro-1,4-naphthoquinone exhibits antimicrobial, anticancer, and antifungal activities. The compound's ability to generate reactive oxygen species has been characterized in various cell-free systems. Its cytotoxic effects on cancer cell lines have been evaluated using cell viability assays. The compound's antimicrobial activity has been assessed using standard disc diffusion or broth microdilution methods. These in vitro findings support its potential applications in antimicrobial and anticancer research.
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| ln Vivo |
In vivo studies of 2-Chloro-1,4-naphthoquinone are limited, as the compound is primarily used as a research chemical and chemical intermediate. Its antimicrobial and anticancer activities suggest potential for in vivo evaluation in infection and cancer models. Naphthoquinone derivatives have been studied in animal models for their therapeutic potential. However, comprehensive in vivo studies specifically targeting 2-Chloro-1,4-naphthoquinone are not well documented in the available literature. Further research is needed to fully characterize its in vivo efficacy and safety.
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| Enzyme Assay |
In vitro enzyme and receptor binding assays for 2-Chloro-1,4-naphthoquinone typically involve testing its redox activity and enzyme inhibition. The compound's ability to generate reactive oxygen species is measured using fluorescent or colorimetric probes. Enzyme inhibition assays are performed for potential targets such as kinases or dehydrogenases. For antimicrobial activity, standard disc diffusion or broth microdilution methods are employed to determine minimum inhibitory concentrations (MICs). All assays are performed with appropriate controls and standardized protocols to ensure reproducibility of results.
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| Cell Assay |
In vitro cell-based assays for 2-Chloro-1,4-naphthoquinone involve culturing cancer cell lines to evaluate its anticancer activity. Cells are treated with varying concentrations of the compound for specified durations (24-72 hours). Cell viability is assessed using MTT, CCK-8, or similar colorimetric assays. Reactive oxygen species levels are measured using fluorescent probes. Apoptosis is quantified using flow cytometry with Annexin V/PI staining or via caspase activity measurements. For antimicrobial studies, bacterial or fungal cultures are treated and cell viability is monitored. All experiments are performed in triplicate with appropriate controls.
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| Animal Protocol |
In vivo animal experiments for 2-Chloro-1,4-naphthoquinone would be conducted to evaluate its antimicrobial and anticancer activities. For antimicrobial studies, infected animals would be treated and pathogen load assessed. For anticancer studies, tumor-bearing animals would be treated and tumor growth monitored. Parameters assessed would include body weight, organ weights, survival, and histopathology. Control groups receiving vehicle alone would be included for comparison. All procedures would comply with institutional animal care and use committee guidelines. Comprehensive in vivo studies are not well documented in the available literature.
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| ADME/Pharmacokinetics |
The pharmacokinetic properties of 2-Chloro-1,4-naphthoquinone reflect its nature as a small quinone compound. It has a molecular weight consistent with its formula C10H5ClO2. As a naphthoquinone, it would have moderate lipophilicity that facilitates absorption and distribution. The compound is expected to be metabolized through redox reactions and conjugation pathways in the liver. Complete pharmacokinetic profiling including half-life, clearance, volume of distribution, and bioavailability would require further systematic studies using appropriate analytical methods such as high-performance liquid chromatography-mass spectrometry.
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| Toxicity/Toxicokinetics |
The toxicity profile of 2-Chloro-1,4-naphthoquinone has been evaluated in the context of its use as a research chemical. As a quinone compound, it can generate reactive oxygen species and cause oxidative stress, which may contribute to cytotoxicity. Proper handling procedures including use of personal protective equipment are recommended when working with pure compound. The compound is not approved for human therapeutic use and is intended for research purposes only. Long-term toxicity studies would be needed to fully establish its safety profile. Naphthoquinones should be handled with caution due to potential toxicity.
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| References |
[1]. Dr Anton Wagner, et al. Process for the preparation of 2-chloronaphthoquinone- (1, 4). Patent. DE1182648B.
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| Additional Infomation |
2-Chloro-1,4-Naphthoquinone is an organochlorine compound belonging to the 1,4-naphthoquinone class of compounds.
2-Chloro-1,4-naphthoquinone (CAS# 1010-60-2) is a naphthoquinone derivative with the molecular formula C10H5ClO2. It is a quinone compound featuring a chlorine substituent at the 2 position of the 1,4-naphthoquinone core. The compound is used as a chemical intermediate in organic synthesis and pharmaceutical research. Naphthoquinones are known for various biological activities including antimicrobial, anticancer, and antifungal properties. 2-Chloro-1,4-naphthoquinone is intended for research use only and is not for human therapeutic use. |
| Molecular Formula |
C10H5CLO2
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|---|---|
| Molecular Weight |
192.60
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| Exact Mass |
191.997
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| CAS # |
1010-60-2
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| PubChem CID |
13891
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| Appearance |
Light yellow to yellow solid powder
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| Density |
1.4±0.1 g/cm3
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| Boiling Point |
308.0±42.0 °C at 760 mmHg
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| Melting Point |
108-109°C
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| Flash Point |
129.3±28.5 °C
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| Vapour Pressure |
0.0±0.7 mmHg at 25°C
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| Index of Refraction |
1.626
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| LogP |
1.98
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
2
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| Rotatable Bond Count |
0
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| Heavy Atom Count |
13
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| Complexity |
293
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| Defined Atom Stereocenter Count |
0
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| SMILES |
ClC1=C([H])C(C2=C([H])C([H])=C([H])C([H])=C2C1=O)=O
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| InChi Key |
CCTJHVLTAJTPBV-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C10H5ClO2/c11-8-5-9(12)6-3-1-2-4-7(6)10(8)13/h1-5H
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| Chemical Name |
2-chloronaphthalene-1,4-dione
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.1921 mL | 25.9605 mL | 51.9211 mL | |
| 5 mM | 1.0384 mL | 5.1921 mL | 10.3842 mL | |
| 10 mM | 0.5192 mL | 2.5961 mL | 5.1921 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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