| Size | Price | Stock | Qty |
|---|---|---|---|
| 500mg |
|
||
| Other Sizes |
| Targets |
2,5-Dimethoxybenzoic acid targets various biological pathways depending on its derivatives. As a benzoic acid derivative, it may interact with enzymes involved in aromatic acid metabolism. The compound's methoxy groups provide electron-donating properties that can influence its biological activity. It may exhibit antimicrobial properties through interactions with microbial cell components. Its antioxidant activity suggests interactions with oxidative stress pathways. The compound's role as a chemical intermediate suggests it can be used to generate compounds with diverse biological targets. Further research is needed to identify its specific molecular targets.
|
|---|---|
| ln Vitro |
In vitro studies have demonstrated that 2,5-Dimethoxybenzoic acid exhibits potential antimicrobial and antioxidant activities. Its ability to scavenge free radicals has been evaluated using cell-free systems such as DPPH radical scavenging assays. The compound's antimicrobial activity has been assessed using standard disc diffusion or broth microdilution methods. As a chemical intermediate, its reactivity and stability have been characterized. These in vitro studies provide foundational data for understanding the compound's properties and its potential applications in pharmaceutical research and organic synthesis.
|
| ln Vivo |
In vivo studies of 2,5-Dimethoxybenzoic acid are limited, as the compound is primarily used as a research chemical and chemical intermediate. Its antioxidant and antimicrobial properties suggest potential for in vivo evaluation in models of oxidative stress and infection. As a benzoic acid derivative, it would be absorbed through the gastrointestinal tract and metabolized through standard pathways for benzoic acid derivatives. Further research is needed to fully characterize its in vivo pharmacokinetic and pharmacodynamic properties.
|
| Enzyme Assay |
In vitro enzyme and receptor binding assays for 2,5-Dimethoxybenzoic acid typically involve testing its antioxidant and antimicrobial activities. Antioxidant activity is assessed using cell-free systems such as DPPH radical scavenging assays, ABTS assays, or ferric reducing antioxidant power (FRAP) assays. For antimicrobial activity, standard disc diffusion or broth microdilution methods are employed to determine minimum inhibitory concentrations (MICs). The compound's purity and identity are assessed using analytical chemistry methods such as nuclear magnetic resonance spectroscopy, high-performance liquid chromatography, and mass spectrometry. All assays are performed with appropriate controls.
|
| Cell Assay |
In vitro cell-based assays for 2,5-Dimethoxybenzoic acid involve culturing cells to evaluate its antioxidant and potential other biological effects. Cells are treated with varying concentrations of the compound and then exposed to oxidative stress inducers such as H2O2. Reactive oxygen species levels are measured using fluorescent probes. Cell viability is assessed using MTT or similar colorimetric assays. For antimicrobial studies, bacterial or fungal cultures are treated with the compound and cell viability is monitored. All experiments are performed in triplicate with appropriate controls to ensure statistical reliability.
|
| Animal Protocol |
In vivo animal experiments for 2,5-Dimethoxybenzoic acid would be conducted to evaluate its antioxidant and antimicrobial activities. For antioxidant studies, animals would be administered the compound and markers of oxidative stress measured in blood and tissue samples. For antimicrobial studies, infected animals would be treated and pathogen load assessed. Parameters assessed would include body weight, organ weights, and histopathology. Control groups receiving vehicle alone would be included for comparison. All procedures would comply with institutional animal care and use committee guidelines. Comprehensive in vivo studies are not well documented in the available literature.
|
| ADME/Pharmacokinetics |
The pharmacokinetic properties of 2,5-Dimethoxybenzoic acid reflect its nature as a small benzoic acid derivative. It has a molecular weight consistent with its formula C9H10O4. As a carboxylic acid, it would be absorbed through the gastrointestinal tract and metabolized through standard pathways for benzoic acid derivatives, including conjugation reactions. Its LogP and other physicochemical properties suggest moderate lipophilicity. Complete pharmacokinetic profiling including half-life, clearance, volume of distribution, and bioavailability would require further systematic studies.
|
| Toxicity/Toxicokinetics |
The toxicity profile of 2,5-Dimethoxybenzoic acid has been evaluated in the context of its use as a research chemical. As a benzoic acid derivative, it may have irritant properties. Proper handling procedures including use of personal protective equipment are recommended when working with pure compound. The compound is not approved for human therapeutic use and is intended for research purposes only. Long-term toxicity studies would be needed to fully establish its safety profile. The compound's methoxy groups may influence its metabolism and toxicity.
|
| References | |
| Additional Infomation |
2,5-Dimethoxybenzoic acid (CAS# 2785-98-0) is a benzoic acid derivative with the molecular formula C9H10O4. It features two methoxy groups at the 2 and 5 positions of the benzene ring and a carboxylic acid group. The compound is used as a chemical intermediate in organic synthesis and pharmaceutical research. As a dimethoxybenzoic acid, it exhibits potential biological activities including antimicrobial and antioxidant properties. 2,5-Dimethoxybenzoic acid is used in research applications for studying phenolic compounds and their biological activities. It is intended for research use only and is not for human therapeutic use.
|
| Molecular Formula |
C9H10O4
|
|---|---|
| Molecular Weight |
182.17
|
| Exact Mass |
182.057
|
| CAS # |
2785-98-0
|
| PubChem CID |
76027
|
| Appearance |
White to light yellow solid
|
| Density |
1.2±0.1 g/cm3
|
| Boiling Point |
329.5±22.0 °C at 760 mmHg
|
| Melting Point |
74-78 °C
|
| Flash Point |
133.6±15.8 °C
|
| Vapour Pressure |
0.0±0.8 mmHg at 25°C
|
| Index of Refraction |
1.533
|
| LogP |
1.67
|
| Hydrogen Bond Donor Count |
1
|
| Hydrogen Bond Acceptor Count |
4
|
| Rotatable Bond Count |
3
|
| Heavy Atom Count |
13
|
| Complexity |
181
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
O(C([H])([H])[H])C1C([H])=C([H])C(=C([H])C=1C(=O)O[H])OC([H])([H])[H]
|
| InChi Key |
NYJBTJMNTNCTCP-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C9H10O4/c1-12-6-3-4-8(13-2)7(5-6)9(10)11/h3-5H,1-2H3,(H,10,11)
|
| Chemical Name |
2,5-Dimethoxybenzoic acid
|
| Synonyms |
2,5-Dimethoxybenzoic acid
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.4894 mL | 27.4469 mL | 54.8938 mL | |
| 5 mM | 1.0979 mL | 5.4894 mL | 10.9788 mL | |
| 10 mM | 0.5489 mL | 2.7447 mL | 5.4894 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
