| Size | Price | Stock | Qty |
|---|---|---|---|
| 100mg |
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| Other Sizes |
| Targets |
IC50: synthesis of long chain fatty acids[1]
Metazachlor targets plant cell division and growth by inhibiting lipid synthesis and microtubule assembly. As a chloroacetanilide herbicide, it interferes with very-long-chain fatty acid synthesis in plants, disrupting cell membrane formation and cell division. The compound's herbicidal activity is selective for certain weed species. Its targets are plant-specific enzymes involved in fatty acid elongation. The compound is not intended for human therapeutic use. |
|---|---|
| ln Vitro |
In vitro studies of Metazachlor have focused on its herbicidal activity and mechanism of action. The compound's inhibition of very-long-chain fatty acid synthesis has been characterized in plant systems. Its effects on cell division and microtubule assembly have been studied. These in vitro findings support its applications in herbicide research and weed management studies.
|
| ln Vivo |
In vivo studies of Metazachlor have demonstrated its efficacy as a herbicide for weed control in various crops. The compound is applied to soil or foliage and inhibits weed growth through its effects on cell division and lipid synthesis. Its selectivity for certain weed species over crop plants has been characterized. The compound is used in agricultural research for studying herbicide mechanisms and weed management.
|
| Enzyme Assay |
In vitro enzyme assays for Metazachlor typically involve testing its inhibition of very-long-chain fatty acid synthesis in plant systems. Enzyme activity is measured by monitoring fatty acid elongation using chromatographic methods. The compound's effects on cell division are assessed using plant cell cultures. All assays are performed with appropriate controls and standardized protocols.
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| Cell Assay |
In vitro cell-based assays for Metazachlor involve culturing plant cells to evaluate its herbicidal activity. Plant cells are treated with varying concentrations of the compound and cell viability is assessed. Effects on cell division and microtubule assembly are monitored using microscopy. All experiments are performed with appropriate controls to ensure statistical reliability.
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| Animal Protocol |
In vivo animal experiments for Metazachlor are conducted for toxicology and environmental safety assessment. Animals are administered the compound and observed for signs of toxicity. Parameters assessed include body weight, organ weights, and histopathology. The compound's environmental fate and effects on non-target organisms are also studied. Control groups receiving vehicle alone are included for comparison. All procedures comply with institutional animal care and use committee guidelines.
|
| ADME/Pharmacokinetics |
The pharmacokinetic properties of Metazachlor reflect its nature as a herbicide. It has a molecular formula of C14H16ClN3O. As a small lipophilic molecule, it is absorbed through plant roots and foliage. The compound is metabolized through standard xenobiotic pathways in plants and animals. Complete pharmacokinetic profiling would require further systematic studies.
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| Toxicity/Toxicokinetics |
The toxicity profile of Metazachlor has been evaluated for environmental and occupational safety. As a herbicide, it may have toxic effects on non-target organisms including aquatic life. Proper handling procedures including use of personal protective equipment are recommended when working with pure compound. The compound is not approved for human therapeutic use and is intended for research purposes only.
|
| References |
[1]. Christiane Wiemann, et al. Metazachlor: Mode of action analysis for rat liver tumour formation and human relevance. Toxicology. 2019 Oct 1;426:152282.
|
| Additional Infomation |
Metazachlor is an organochlorine compound with the structure 2-chloroacetamide, where the nitrogen atom is replaced by a 2,6-dimethylphenyl and a (1H-pyrazol-1-ylmethyl) group. It is an environmental pollutant, an exogenous substance, and a herbicide. Metazachlor is an aromatic amide, an organochlorine compound, and belongs to the pyrazole class of compounds.
Metazachlor (methoxychloramine, CAS# 67129-08-2) is a chloroacetanilide herbicide used for weed control in various crops. It has the molecular formula C14H16ClN3O. Metazachlor inhibits cell division and growth in susceptible plants by interfering with lipid synthesis and microtubule assembly. The compound is used in agricultural research for studying herbicide mechanisms and weed management. It is intended for research use only. |
| Molecular Formula |
C14H16CLN3O
|
|---|---|
| Molecular Weight |
277.75
|
| Exact Mass |
277.098
|
| CAS # |
67129-08-2
|
| PubChem CID |
49384
|
| Appearance |
Typically exists as solid at room temperature
|
| Density |
1.19 g/cm3
|
| Boiling Point |
439.2ºC at 760 mmHg
|
| Melting Point |
74-78°C
|
| Flash Point |
2 °C
|
| Index of Refraction |
1.586
|
| LogP |
2.729
|
| Hydrogen Bond Donor Count |
0
|
| Hydrogen Bond Acceptor Count |
2
|
| Rotatable Bond Count |
4
|
| Heavy Atom Count |
19
|
| Complexity |
303
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
ClC([H])([H])C(N(C([H])([H])N1C([H])=C([H])C([H])=N1)C1C(C([H])([H])[H])=C([H])C([H])=C([H])C=1C([H])([H])[H])=O
|
| InChi Key |
STEPQTYSZVCJPV-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C14H16ClN3O/c1-11-5-3-6-12(2)14(11)18(13(19)9-15)10-17-8-4-7-16-17/h3-8H,9-10H2,1-2H3
|
| Chemical Name |
2-chloro-N-(2,6-dimethylphenyl)-N-(pyrazol-1-ylmethyl)acetamide
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO: 100 mg/mL (360.04 mM)
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.6004 mL | 18.0018 mL | 36.0036 mL | |
| 5 mM | 0.7201 mL | 3.6004 mL | 7.2007 mL | |
| 10 mM | 0.3600 mL | 1.8002 mL | 3.6004 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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