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| Targets |
Poriol targets pathways involved in plant defense responses, as it is produced in reaction to infection by Poria weirii in Douglas fir. As a flavonoid, it may interact with various biological targets including enzymes, receptors, and signaling pathways involved in inflammation and infection. The compound's C-methylated flavanone structure provides specific structural features for biological interactions. Its use as an anti-infection agent suggests it may have antimicrobial or antifungal properties. Further research is needed to identify its specific molecular targets and mechanisms of action.
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| ln Vitro |
In vitro studies of Poriol have focused on its isolation and characterization from various plant species. The compound has been isolated from Pseudotsuga sinensis, Cephalotaxus sinensis, and Pinus morrisonicola. Its chemical structure has been confirmed as a C-methylated flavanone. The compound is used as an anti-infection agent in research applications. Its purity of ≥98% supports its use in research applications. These in vitro studies provide foundational data for understanding the compound's properties and its potential applications in anti-infection research.
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| ln Vivo |
In vivo studies of Poriol are limited as the compound is primarily used as a research chemical. Its production in Douglas fir in response to Poria weirii infection suggests it plays a role in plant defense mechanisms in vivo. As a flavonoid, it may have biological activities including anti-infection properties. However, comprehensive in vivo pharmacological studies specifically targeting Poriol are not well documented in the available literature. The compound is intended for research use only and is not for human therapeutic use.
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| Enzyme Assay |
In vitro enzyme assays for Poriol typically involve testing its anti-infection activity. Antimicrobial activity is assessed using standard disc diffusion or broth microdilution methods to determine minimum inhibitory concentrations (MICs) against various bacterial and fungal strains. The compound's purity and identity are assessed using analytical chemistry methods such as high-performance liquid chromatography, nuclear magnetic resonance spectroscopy, and mass spectrometry. Antioxidant activity may be assessed using cell-free systems such as DPPH radical scavenging assays. All assays are performed with appropriate controls and standardized protocols.
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| Cell Assay |
In vitro cell-based assays for Poriol involve culturing cells to evaluate its anti-infection and potential other biological activities. Cells are treated with varying concentrations of the compound and cell viability is assessed using MTT or similar colorimetric assays. For antimicrobial studies, bacterial or fungal cultures are treated with the compound and cell viability is monitored. For anti-inflammatory studies, immune cells are treated with the compound and stimulated with inflammatory stimuli; cytokine production is measured by ELISA. All experiments are performed in triplicate with appropriate controls to ensure statistical reliability.
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| Animal Protocol |
In vivo animal experiments for Poriol would be conducted to evaluate its anti-infection activities. For antimicrobial studies, infected animals would be treated with the compound and pathogen load assessed. For anti-inflammatory studies, animals with induced inflammation would be treated and inflammatory markers measured. Parameters assessed would include body weight, organ weights, and histopathology. Control groups receiving vehicle alone would be included for comparison. All procedures would comply with institutional animal care and use committee guidelines. Comprehensive in vivo studies are not well documented in the available literature.
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| ADME/Pharmacokinetics |
The pharmacokinetic properties of Poriol reflect its nature as a flavonoid compound. It has a molecular weight of 286.28 and the molecular formula C16H14O5. The compound is soluble in chloroform, dichloromethane, ethyl acetate, DMSO, and acetone, indicating moderate lipophilicity. It is stored desiccated at -20°C. As a flavonoid, it would be absorbed through the gastrointestinal tract and metabolized through standard xenobiotic pathways in the liver. Complete pharmacokinetic profiling including half-life, clearance, volume of distribution, and bioavailability would require further systematic studies.
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| Toxicity/Toxicokinetics |
The toxicity profile of Poriol has been evaluated in the context of its use as a research chemical. As a natural flavonoid found in various plant species, it is expected to have a favorable safety profile at research concentrations. Proper handling procedures including use of personal protective equipment are recommended when working with pure compound. The compound is not approved for human therapeutic use and is intended for research purposes only. Long-term toxicity studies would be needed to fully establish its safety profile for pharmaceutical applications.
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| References | |
| Additional Infomation |
Poriol is a type of flavanone compound. It has been reported that Poriol is present in Pinus morrisonicola, and relevant data are available for reference.
Poriol (CAS# 14348-16-4) is a flavonoid compound belonging to the flavanone subclass, with the molecular formula C16H14O5 and a molecular weight of 286.28. It is a C-methylated flavanone isolated from Pseudotsuga sinensis, Cephalotaxus sinensis, and Populus species. It is found in Pinus morrisonicola and is produced in reaction to Poria weirii infection in Douglas fir. The compound has a purity of ≥98% and is soluble in various organic solvents. Poriol is used as an anti-infection agent in research applications. It is intended for research use only. |
| Molecular Formula |
C16H14O5
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| Molecular Weight |
286.28
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| Exact Mass |
286.084
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| CAS # |
14348-16-4
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| PubChem CID |
301798
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| Appearance |
White to off-white solid
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| Density |
1.434g/cm3
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| Boiling Point |
588.6ºC at 760mmHg
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| Flash Point |
225.4ºC
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| Vapour Pressure |
1.89E-14mmHg at 25°C
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| Index of Refraction |
1.676
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| LogP |
2.818
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
1
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| Heavy Atom Count |
21
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| Complexity |
390
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| Defined Atom Stereocenter Count |
0
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| SMILES |
OC1C=CC(C2CC(=O)C3C(=C(C(=CC=3O2)O)C)O)=CC=1
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| InChi Key |
SLFZBNOERHGNMI-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C16H14O5/c1-8-11(18)6-14-15(16(8)20)12(19)7-13(21-14)9-2-4-10(17)5-3-9/h2-6,13,17-18,20H,7H2,1H3
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| Chemical Name |
5,7-dihydroxy-2-(4-hydroxyphenyl)-6-methyl-2,3-dihydrochromen-4-one
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| Synonyms |
Poriol
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.4931 mL | 17.4654 mL | 34.9308 mL | |
| 5 mM | 0.6986 mL | 3.4931 mL | 6.9862 mL | |
| 10 mM | 0.3493 mL | 1.7465 mL | 3.4931 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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