| Size | Price | Stock | Qty |
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| 50mg |
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| 100mg |
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| Other Sizes |
| Targets |
Dibenzyl hydrazodicarboxylate targets proteins as a cross-linking agent. As a protected hydrazine derivative, it can be used to crosslink proteins through its reactive functional groups. The compound's two benzyloxycarbonyl protecting groups provide stability during synthesis and can be removed under appropriate conditions. Its targets are chemical rather than biological, as it is primarily used as a research reagent for protein crosslinking and organic synthesis.
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| ln Vitro |
In vitro studies of Dibenzyl hydrazodicarboxylate have focused on its role as a protein cross-linking agent and protected hydrazine derivative. The compound's reactivity and stability have been characterized in various chemical reactions. Its purity (99%) and identity are confirmed using analytical chemistry methods such as nuclear magnetic resonance spectroscopy, high-performance liquid chromatography, and mass spectrometry. These in vitro studies provide foundational data for understanding the compound's utility in protein chemistry and organic synthesis.
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| ln Vivo |
In vivo studies of Dibenzyl hydrazodicarboxylate are not applicable as the compound is primarily used as a research reagent for chemical synthesis rather than as a therapeutic agent. The compound is not intended for administration to living organisms. Its applications are limited to chemical research and protein chemistry. The compound is not approved for human therapeutic use and is intended for research purposes only.
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| Enzyme Assay |
In vitro chemical assays for Dibenzyl hydrazodicarboxylate involve testing its reactivity and purity as a chemical reagent. The compound's purity (99%) and identity are confirmed using analytical chemistry methods such as nuclear magnetic resonance spectroscopy, high-performance liquid chromatography, and mass spectrometry. Its reactivity in protein crosslinking reactions is evaluated using standard biochemical techniques. All assays are performed with appropriate controls and standardized protocols.
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| Cell Assay |
In vitro cell-based assays are not applicable for Dibenzyl hydrazodicarboxylate as the compound is a chemical reagent used for protein crosslinking rather than a biological agent. The compound is not intended for cell-based studies as a direct pharmacological agent. Its applications are limited to chemical research and protein chemistry. Cell-based assays may be used to evaluate the effects of proteins crosslinked with this reagent, but the reagent itself is not tested in cell-based systems.
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| Animal Protocol |
In vivo animal experiments are not applicable for Dibenzyl hydrazodicarboxylate as the compound is a chemical reagent used for research rather than a therapeutic agent. The compound is not administered to animals for pharmacological evaluation. Its applications are limited to chemical research and protein chemistry. The compound is not intended for in vivo use.
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| ADME/Pharmacokinetics |
The pharmacokinetic properties of Dibenzyl hydrazodicarboxylate are not applicable as the compound is a chemical reagent used for research rather than a therapeutic agent. It has a molecular weight of 300.31 and the molecular formula C16H16N2O4. The compound has a density of 1.2±0.1 g/cm3 and a boiling point of 462.4±34.0 °C. It is stored at -20°C. Its physicochemical properties are characterized for its use as a chemical reagent.
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| Toxicity/Toxicokinetics |
The toxicity profile of Dibenzyl hydrazodicarboxylate has been evaluated in the context of its use as a research chemical. As a protected hydrazine derivative, it may have irritant properties. The compound has a purity of 99%. Proper handling procedures including use of personal protective equipment are recommended when working with pure compound. The compound is not approved for human therapeutic use and is intended for research purposes only.
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| References | |
| Additional Infomation |
Dibenzyl hydrazodicarboxylate (CAS# 5394-50-3) is also known as dibenzyl hydrazine-1,2-dicarboxylate, 1,2-dicarbobenzyloxyhydrazine. It has the molecular formula C16H16N2O4 and a molecular weight of 300.31. The compound is a protected hydrazine derivative that serves as a stable reagent. Dibenzyl hydrazodicarboxylate is a protein cross-linking agent. It has a purity of 99% and is stored at -20°C.
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| Molecular Formula |
C16H16N2O4
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|---|---|
| Molecular Weight |
300.31
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| Exact Mass |
300.111
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| CAS # |
5394-50-3
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| PubChem CID |
220554
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| Appearance |
White to off-white solid powder
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| Density |
1.248g/cm3
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| Boiling Point |
462.4ºC at 760mmHg
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| Flash Point |
233.5ºC
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| Index of Refraction |
1.582
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| LogP |
3.536
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
6
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| Heavy Atom Count |
22
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| Complexity |
316
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| Defined Atom Stereocenter Count |
0
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| SMILES |
C1=CC=C(C=C1)COC(=O)NNC(=O)OCC2=CC=CC=C2
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| InChi Key |
UEYMRBONTZTXQP-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C16H16N2O4/c19-15(21-11-13-7-3-1-4-8-13)17-18-16(20)22-12-14-9-5-2-6-10-14/h1-10H,11-12H2,(H,17,19)(H,18,20)
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| Chemical Name |
benzyl N-(phenylmethoxycarbonylamino)carbamate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.3299 mL | 16.6495 mL | 33.2989 mL | |
| 5 mM | 0.6660 mL | 3.3299 mL | 6.6598 mL | |
| 10 mM | 0.3330 mL | 1.6649 mL | 3.3299 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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