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| Other Sizes |
| Targets |
β-Amylase targets starch and glycogen as substrates, catalyzing the hydrolysis of α-1,4-glucoside bonds. The enzyme acts on the non-reducing ends of amylose and amylopectin chains, breaking them down into maltose and glucose. β-Amylase is used in various biochemical studies including starch degradation research. Its targets are polysaccharide substrates rather than biological receptors. The enzyme is classified as a metabolic enzyme/protease.
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| ln Vitro |
In vitro studies have demonstrated that β-Amylase has abundant starch degrading activities. The enzyme catalyzes the hydrolysis of α-1,4-glucoside bonds in starch and glycogen. It is used as a gel filtration molecular weight marker in chromatography. These in vitro findings support its applications in biochemical research, food processing, and starch hydrolysis studies.
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| ln Vivo |
In vivo studies of β-Amylase are limited as the enzyme is primarily used as a research reagent rather than a therapeutic agent. The enzyme plays a role in starch digestion in plants and microorganisms in vivo. In industrial applications, β-Amylase is used in the production of beer syrup and high maltose syrup. The compound is not intended for human therapeutic use and is intended for research and industrial purposes only.
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| Enzyme Assay |
In vitro enzyme assays for β-Amylase typically involve testing its starch-degrading activity. Enzyme activity is measured by monitoring the release of maltose or glucose from starch substrates using colorimetric or chromatographic methods. The enzyme's molecular weight and purity are assessed using gel filtration chromatography and SDS-PAGE. All assays are performed with appropriate controls and standardized protocols to ensure reproducibility of results.
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| Cell Assay |
In vitro cell-based assays are not typically performed for β-Amylase as the enzyme is used as a research reagent for biochemical studies rather than a biological agent. The enzyme's activity is assessed in cell-free systems using starch or glycogen substrates. Cell-based assays may be used to study the enzyme's effects on cellular glycogen metabolism, but the enzyme itself is not a direct pharmacological agent.
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| Animal Protocol |
In vivo animal experiments for β-Amylase are not applicable as the enzyme is primarily used as a research reagent and industrial enzyme rather than a therapeutic agent. The enzyme is not administered to animals for pharmacological evaluation. Its applications are limited to biochemical research and industrial processes including starch hydrolysis and syrup production.
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| ADME/Pharmacokinetics |
The pharmacokinetic properties of β-Amylase are not applicable as the enzyme is primarily used as a research reagent and industrial enzyme rather than a therapeutic agent. It has a CAS number of 9000-91-3. The enzyme is available in both liquid and powder forms. It is stored at 2-8°C. Its physicochemical properties are characterized for its use as a research and industrial enzyme.
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| Toxicity/Toxicokinetics |
The toxicity profile of β-Amylase has been evaluated in the context of its use as a food processing enzyme and research reagent. The enzyme is generally recognized as safe for food processing applications. Proper handling procedures including use of personal protective equipment are recommended when working with the enzyme. The compound is not approved for human therapeutic use and is intended for research and industrial purposes only.
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| References | |
| Additional Infomation |
β-Amylase (CAS# 9000-91-3) is also known as 1,4-α-D-glucan maltohydrolase, glycogenase. It is an enzyme that catalyzes the hydrolysis of α-1,4-glucoside bonds in starch and glycogen to produce maltose and glucose. β-Amylase has abundant starch degrading activities and is used for various biochemical studies. It is a gel filtration molecular weight marker used in chromatography. The enzyme is used in beer syrup and high maltose syrup production.
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| Molecular Formula |
C31H27NO4
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|---|---|
| Molecular Weight |
477.5503885746
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| Exact Mass |
477.194
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| CAS # |
9000-91-3
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| Appearance |
White to off-white solid powder
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| Density |
1.2±0.1 g/cm3
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| Boiling Point |
643.1±55.0 °C at 760 mmHg
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| Flash Point |
342.7±31.5 °C
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| Vapour Pressure |
0.0±1.9 mmHg at 25°C
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| Index of Refraction |
1.639
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| LogP |
7.16
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| SMILES |
O(CC1C=CC=CC=1)C(C1=C(C2C=CC=CC=2)N([H])C(C)=C(C(=O)OCC)C1C#CC1C=CC=CC=1)=O
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.0940 mL | 10.4701 mL | 20.9402 mL | |
| 5 mM | 0.4188 mL | 2.0940 mL | 4.1880 mL | |
| 10 mM | 0.2094 mL | 1.0470 mL | 2.0940 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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