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| Other Sizes |
| Targets |
Tryptone targets microbial growth and metabolism by providing essential nutrients, particularly nitrogen and carbon sources. As a mixture of peptides and amino acids, it serves as a substrate for microbial protein synthesis and energy metabolism. The compound's various amino acids are utilized by bacteria and yeast for growth and protein expression. Tryptone is also used as a flavoring agent and as an inert ingredient in non-food pesticide products. Its targets are microbial metabolic pathways rather than specific biological receptors.
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| ln Vitro |
In vitro studies of Tryptone have focused on its role as a microbial culture medium component. The compound provides nutrients needed for bacterial growth, especially nitrogen and carbon sources. It is widely used in LB medium, liquid culture, and protein expression research. Tryptone's various amino acids support the growth of a wide range of microorganisms. These in vitro studies provide foundational data for understanding the compound's utility in microbiology and biotechnology research.
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| ln Vivo |
In vivo studies of Tryptone are limited as the compound is primarily used as a laboratory reagent for microbial culture rather than as a therapeutic agent. The compound is not intended for administration to living organisms for pharmacological evaluation. Its applications are limited to microbiological research and biotechnology. Tryptone is permitted for use as an inert ingredient in non-food pesticide products. The compound is not approved for human therapeutic use and is intended for research purposes only.
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| Enzyme Assay |
In vitro enzyme assays are not typically performed for Tryptone as the compound is a mixture of peptides and amino acids used as a microbial culture medium component rather than a biological agent. Its nutrient composition is assessed using analytical chemistry methods. The compound's ability to support microbial growth is evaluated using standard microbiological techniques including optical density measurements and colony counting. All assays are performed with appropriate controls and standardized protocols to ensure reproducibility of results.
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| Cell Assay |
In vitro cell-based assays for Tryptone involve culturing bacteria or yeast to evaluate its ability to support growth and protein expression. Microorganisms are cultured in media containing Tryptone and growth is monitored by optical density measurements. Protein expression is assessed using standard molecular biology techniques such as SDS-PAGE and Western blotting. The compound's nutrient composition supports the growth of a wide range of microorganisms. All experiments are performed with appropriate controls to ensure statistical reliability.
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| Animal Protocol |
In vivo animal experiments for Tryptone are not applicable as the compound is primarily used as a laboratory reagent for microbial culture rather than as a therapeutic agent. The compound is not administered to animals for pharmacological evaluation. Its applications are limited to microbiological research and biotechnology. The compound is not intended for in vivo use. All procedures involving the compound are conducted in laboratory settings for research purposes only.
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| ADME/Pharmacokinetics |
The pharmacokinetic properties of Tryptone are not applicable as the compound is a mixture of peptides and amino acids used as a microbial culture medium component rather than a therapeutic agent. It has a CAS number of 73049-73-7 and an EINECS number of 293-432-6. The compound appears as a solid and is soluble in water at 100 mg/mL. It is stored as a powder at -20°C for 3 years or at 4°C for 2 years. The compound's physicochemical properties are characterized for its use as a laboratory reagent.
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| Toxicity/Toxicokinetics |
The toxicity profile of Tryptone has been evaluated in the context of its use as a laboratory reagent. As a mixture of peptides and amino acids derived from casein hydrolysis, it is generally recognized as safe for laboratory use. The compound is permitted for use as an inert ingredient in non-food pesticide products. Proper handling procedures including use of personal protective equipment are recommended when working with the compound. The compound is not approved for human therapeutic use and is intended for research purposes only.
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| References |
[1]. Liana AE, et al. T4 bacteriophage conjugated magnetic particles for E. coli capturing: Influence of bacteriophage loading, temperature and tryptone. Colloids Surf B Biointerfaces. 2017 Mar 1;151:47-57.
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| Additional Infomation |
See also: Peptone (note moved to).
Tryptone (CAS# 73049-73-7) is a mixture of peptides produced by the hydrolysis of proteins (typically casein) using bovine trypsin. It contains a variety of short peptides and amino acids. Tryptone is a common component of microbial culture media, providing a nitrogen source and growth factors for bacteria and yeast. It is widely used in LB medium, liquid culture, and protein expression research. The compound is soluble in water at 100 mg/mL and is stored as a powder. It is intended for research use only. |
| Molecular Weight |
0
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|---|---|
| Exact Mass |
244.167
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| CAS # |
73049-73-7
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| PubChem CID |
42552953
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| Appearance |
Yellow to brown solid powder
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| Density |
1.0±0.1 g/cm3
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| Boiling Point |
253.0±8.0 °C at 760 mmHg
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| Flash Point |
107.5±16.9 °C
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| Vapour Pressure |
0.0±0.5 mmHg at 25°C
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| Index of Refraction |
1.438
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| LogP |
3.34
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
9
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| Heavy Atom Count |
17
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| Complexity |
240
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| Defined Atom Stereocenter Count |
0
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| SMILES |
N1C=NN=C1
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| InChi Key |
AIUDWMLXCFRVDR-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C13H24O4/c1-6-13(3,7-2)9-8-10(11(14)16-4)12(15)17-5/h10H,6-9H2,1-5H3
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| Chemical Name |
dimethyl 2-(3-ethyl-3-methylpentyl)propanedioate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O: 100 mg/mL
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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