| Size | Price | |
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| Other Sizes |
| Targets |
DEAE-Sephadex A 25 targets negatively charged biomolecules through ion exchange interactions. The diethylaminoethyl groups on the Sephadex matrix provide positively charged binding sites that interact with negatively charged molecules such as proteins, nucleic acids, and polysaccharides. The compound's targets are chemical rather than biological, as it is used as a chromatography medium for biomolecule separation. Its applications are limited to biochemical research and purification.
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| ln Vitro |
In vitro studies of DEAE-Sephadex A 25 have focused on its role as an ion exchange chromatography medium. The compound's ability to bind and separate biomolecules based on charge has been characterized. It is widely used for protein purification, enzyme isolation, and nucleic acid separation. These in vitro studies provide foundational data for understanding the compound's utility in biochemical research and purification applications.
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| ln Vivo |
In vivo studies of DEAE-Sephadex A 25 are not applicable as the compound is a chromatography medium used for in vitro biomolecule separation. The compound is not intended for administration to living organisms. Its applications are limited to biochemical research and purification. The compound is not approved for human therapeutic use and is intended for research purposes only.
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| Enzyme Assay |
In vitro chromatography assays for DEAE-Sephadex A 25 typically involve testing its ability to bind and separate biomolecules. Proteins, nucleic acids, or polysaccharides are applied to the column and eluted using salt gradients. The compound's binding capacity and resolution are assessed. Its physical properties such as particle size and swelling characteristics are characterized. All assays are performed with appropriate controls and standardized protocols.
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| Cell Assay |
In vitro cell-based assays are not applicable for DEAE-Sephadex A 25 as the compound is a chromatography medium used for biomolecule purification rather than a biological agent. The compound is not intended for cell-based studies as a direct pharmacological agent. Its applications are limited to biochemical research and purification. Cell-based assays may be used to evaluate the biological activity of proteins purified using this medium, but the medium itself is not tested in cell-based systems.
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| Animal Protocol |
In vivo animal experiments are not applicable for DEAE-Sephadex A 25 as the compound is a chromatography medium used for in vitro biomolecule purification rather than a therapeutic agent. The compound is not administered to animals for pharmacological evaluation. Its applications are limited to biochemical research and purification. The compound is not intended for in vivo use.
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| ADME/Pharmacokinetics |
The pharmacokinetic properties of DEAE-Sephadex A 25 are not applicable as the compound is a chromatography medium used for in vitro biomolecule purification rather than a therapeutic agent. The compound is a cross-linked dextran gel with DEAE functional groups. Its physicochemical properties including particle size, swelling capacity, and ion exchange capacity are characterized for its use as a chromatography medium.
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| Toxicity/Toxicokinetics |
The toxicity profile of DEAE-Sephadex A 25 has been evaluated in the context of its use as a laboratory reagent. As a chromatography medium, it is generally considered safe for laboratory use when handled properly. Proper handling procedures including use of personal protective equipment are recommended when working with the compound. The compound is not approved for human therapeutic use and is intended for research purposes only.
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| References |
[1]. Masataka Hiraide, et al. Evaluation of humic complexes of trace metals in river water by adsorption on indium-treated XAD-2 resin and DEAE-Sephadex A-25 anion exchanger.Fresenius' Journal of Analytical Chemistry volume 348, pages758–761 (1994).
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| Additional Infomation |
DEAE-Sephadex A 25 (CAS# 12609-80-2) is a diethylaminoethyl (DEAE) derivative of Sephadex, a cross-linked dextran gel. It is an ion exchange chromatography medium used for the separation and purification of biomolecules. DEAE-Sephadex A 25 is a weak anion exchanger that binds negatively charged molecules such as proteins, nucleic acids, and polysaccharides. It is widely used in biochemical research for protein purification, enzyme isolation, and nucleic acid separation.
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| Molecular Formula |
C11H9N3O2.NA+
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|---|---|
| Molecular Weight |
238.19786
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| CAS # |
12609-80-2
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| Appearance |
White to off-white solid powder
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.1982 mL | 20.9908 mL | 41.9815 mL | |
| 5 mM | 0.8396 mL | 4.1982 mL | 8.3963 mL | |
| 10 mM | 0.4198 mL | 2.0991 mL | 4.1982 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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