| Size | Price | Stock | Qty |
|---|---|---|---|
| 100mg |
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| Other Sizes |
| Targets |
(E/Z)-4,4'-Dicyanostilbene is the inactive isomer and does not exhibit significant biological activity. It serves as an experimental control for studies involving the active 4,4'-Dicyanostilbene isomer. The active isomer targets malaria parasites (Dd2 strain with EC50 of 27 nM) and methicillin-resistant Staphylococcus aureus (MRSA). The (E/Z) isomer is used as a reference compound in pharmacological research.
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| ln Vitro |
(E/Z)-4,4'-Dicyanostilbene is the inactive isomer and does not exhibit significant biological activity in vitro. It is used as a control compound in experiments. 4,4'-Dicyanostilbene (compound 43) is a potent antimalarial agent with an EC50 of 27 nM against the Dd2 strain. The active isomer also shows activity against MRSA. (E/Z)-4,4'-Dicyanostilbene itself shows no notable activity and is intended for use as an experimental control.
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| ln Vivo |
In vivo studies of (E/Z)-4,4'-Dicyanostilbene are limited as the compound is an inactive isomer used primarily as an experimental control. 4,4'-Dicyanostilbene (compound 43) exhibits in vivo efficacy against methicillin-resistant Staphylococcus aureus (MRSA). The active isomer also shows antimalarial activity. (E/Z)-4,4'-Dicyanostilbene itself does not exhibit significant in vivo activity and is not intended for therapeutic use.
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| Enzyme Assay |
In vitro enzyme/receptor binding assays for (E/Z)-4,4'-Dicyanostilbene typically involve testing its activity as a control compound for the active 4,4'-Dicyanostilbene isomer. The compound's purity (typically ≥98%) and identity are confirmed using analytical chemistry methods such as nuclear magnetic resonance spectroscopy, high-performance liquid chromatography, and mass spectrometry. It is used as an inactive control in binding assays to establish baseline activity.
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| Cell Assay |
In vitro cell-based assays for (E/Z)-4,4'-Dicyanostilbene involve culturing cells to evaluate its effects as a control compound. Cells are treated with varying concentrations of the compound and compared to the active isomer. The compound is used to establish baseline activity and ensure that observed effects are specific to the active isomer. Cell viability is assessed using standard assays. All experiments are performed with appropriate controls to ensure statistical reliability.
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| Animal Protocol |
In vivo animal experiments for (E/Z)-4,4'-Dicyanostilbene are limited as the compound is an inactive isomer used as a control. 4,4'-Dicyanostilbene (compound 43) exhibits in vivo efficacy against MRSA. (E/Z)-4,4'-Dicyanostilbene itself is not intended for in vivo efficacy studies. For control purposes, animals would be administered the compound and compared to active treatment groups. All procedures would comply with institutional animal care and use committee guidelines.
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| ADME/Pharmacokinetics |
(E/Z)-4,4'-Dicyanostilbene has a molecular weight of 230.26 and the molecular formula C16H10N2. The compound has a purity of 98%. As an inactive isomer, it does not exhibit the pharmacokinetic properties of the active isomer. Complete pharmacokinetic profiling of (E/Z)-4,4'-Dicyanostilbene would require further systematic studies, but the compound is primarily used as a control reagent rather than for pharmacokinetic evaluation.
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| Toxicity/Toxicokinetics |
The toxicity profile of (E/Z)-4,4'-Dicyanostilbene is not well documented as the compound is primarily used as an experimental control. As an inactive isomer, it is expected to have lower biological activity and potentially lower toxicity compared to the active isomer. Proper handling procedures including use of personal protective equipment are recommended when working with the compound. The compound is not approved for human therapeutic use and is intended for research purposes only.
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| References |
[1]. Kyei-Baffour K, et, al. Natural product-inspired aryl isonitriles as a new class of antimalarial compounds against drug-resistant parasites. Bioorg Med Chem. 2020 Oct 1;28(19):115678.
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| Additional Infomation |
(E/Z)-4,4'-Dicyanostilbene (CAS# 6292-62-2) has the molecular formula C16H10N2 and a molecular weight of 230.26. The compound is the inactive isomer of 4,4'-Dicyanostilbene and can be used as an experimental control. 4,4'-Dicyanostilbene (compound 43) is a potent antimalarial agent (EC50 = 27 nM) with in vivo efficacy against MRSA.
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| Molecular Formula |
C16H10N2
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|---|---|
| Molecular Weight |
230.26
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| Exact Mass |
230.084
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| CAS # |
6292-62-2
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| Related CAS # |
4,4'-Dicyanostilbene;5216-37-5
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| PubChem CID |
759338
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| Appearance |
White to off-white solid powder
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| Density |
1.17g/cm3
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| Boiling Point |
445ºC at 760 mmHg
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| Flash Point |
218.6ºC
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| Index of Refraction |
1.63
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| LogP |
3.6
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
2
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
18
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| Complexity |
338
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| Defined Atom Stereocenter Count |
0
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| SMILES |
C1=CC(=CC=C1C=CC2=CC=C(C=C2)C#N)C#N
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| InChi Key |
RNIZPDIBRXCMRD-OWOJBTEDSA-N
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| InChi Code |
InChI=1S/C16H10N2/c17-11-15-7-3-13(4-8-15)1-2-14-5-9-16(12-18)10-6-14/h1-10H/b2-1+
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| Chemical Name |
4-[(E)-2-(4-cyanophenyl)ethenyl]benzonitrile
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.3429 mL | 21.7146 mL | 43.4292 mL | |
| 5 mM | 0.8686 mL | 4.3429 mL | 8.6858 mL | |
| 10 mM | 0.4343 mL | 2.1715 mL | 4.3429 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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