| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 10mg | |||
| Other Sizes |
| Targets |
Brassinosteroid receptors (BRI1, BAK1).
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|---|---|
| ln Vitro |
24-Epicastasterone is a brassinosteroid that binds to the brassinosteroid receptor BRI1 and its co-receptor BAK1, activating a signaling cascade that regulates gene expression. This leads to promotion of cell elongation, division, and differentiation. It also enhances plant stress responses, including tolerance to drought, salinity, and temperature extremes. The compound has been used to study the structure-activity relationships of brassinosteroids.
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| ln Vivo |
In vivo studies in plants have demonstrated that 24-epicastasterone promotes growth, enhances stress tolerance, and regulates developmental processes. It is used in agricultural research to evaluate the potential of brassinosteroids for improving crop yield and stress resistance. The compound is applied to plants by foliar spray or root treatment.
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| Enzyme Assay |
Brassinosteroid activity is assessed using the rice lamina inclination assay, a standard bioassay for brassinosteroids. Rice seedlings are treated with the compound and the angle of leaf inclination is measured. Binding assays to BRI1 are conducted using radiolabeled brassinolide or surface plasmon resonance. Gene expression analysis is performed by qPCR to assess the activation of brassinosteroid-responsive genes.
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| Cell Assay |
Plant cell cultures or protoplasts are treated with 24-epicastasterone and gene expression is analyzed. The compound's effects on cell elongation, division, and differentiation can be studied in cultured plant cells.
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| Animal Protocol |
In vivo studies are conducted in plants, including Arabidopsis, rice, and other crop species. Plants are treated with 24-epicastasterone and growth parameters (height, biomass, leaf area) are measured. Stress tolerance is assessed by exposing treated plants to drought, salinity, or temperature stress. Yield parameters are measured in crop species.
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| ADME/Pharmacokinetics |
24-Epicastasterone is a plant hormone and is used in plant physiology research. It is soluble in organic solvents and can be formulated for foliar application or root treatment. Standard laboratory storage conditions apply.
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| Toxicity/Toxicokinetics |
24-Epicastasterone is a plant growth regulator and is not intended for human or animal use. It has low toxicity to mammals. Standard laboratory safety practices should be followed when handling this compound.
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| References | |
| Additional Infomation |
It has been reported that beetroot, Ornithopus sativus, and other organisms with available data contain 24-epistosterone.
24-Epicastasterone is a brassinosteroid used in plant physiology research. It is a stereoisomer of castasterone, a naturally occurring plant hormone. Brassinosteroids regulate plant growth, development, and stress responses. 24-Epicastasterone is used to study the effects of brassinosteroids and their potential applications in agriculture for improving crop yield and stress tolerance. It is for research use only. |
| Molecular Formula |
C28H48O5
|
|---|---|
| Molecular Weight |
464.68
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| Exact Mass |
463.342
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| CAS # |
72050-71-6
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| PubChem CID |
11812633
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| Appearance |
White to off-white solid
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| Density |
1.127g/cm3
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| Boiling Point |
598.7ºC at 760 mmHg
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| Flash Point |
329.9ºC
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| Index of Refraction |
1.541
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| LogP |
3.62
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| Hydrogen Bond Donor Count |
4
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
5
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| Heavy Atom Count |
33
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| Complexity |
738
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| Defined Atom Stereocenter Count |
13
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| SMILES |
C[C@@H]([C@H]1CC[C@@H]2[C@@]1(CC[C@H]3[C@H]2CC(=O)[C@@H]4[C@@]3(C[C@H]([C@H](C4)O)O)C)C)[C@H]([C@@H]([C@H](C)C(C)C)O)O
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| InChi Key |
VYUIKSFYFRVQLF-QONPFPPSSA-N
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| InChi Code |
InChI=1S/C28H48O5/c1-14(2)15(3)25(32)26(33)16(4)18-7-8-19-17-11-22(29)21-12-23(30)24(31)13-28(21,6)20(17)9-10-27(18,19)5/h14-21,23-26,30-33H,7-13H2,1-6H3/t15-,16+,17+,18-,19+,20+,21-,23+,24-,25-,26-,27-,28-/m1/s1
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| Chemical Name |
(2R,3S,5S,8S,9S,10R,13S,14S,17R)-17-[(2S,3R,4R,5R)-3,4-dihydroxy-5,6-dimethylheptan-2-yl]-2,3-dihydroxy-10,13-dimethyl-1,2,3,4,5,7,8,9,11,12,14,15,16,17-tetradecahydrocyclopenta[a]phenanthren-6-one
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| Synonyms |
24-epi-Castasterone; 24-Epicastasterone
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
THF: 10 mg/mL (21.52 mM)
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.1520 mL | 10.7601 mL | 21.5202 mL | |
| 5 mM | 0.4304 mL | 2.1520 mL | 4.3040 mL | |
| 10 mM | 0.2152 mL | 1.0760 mL | 2.1520 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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