| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| 5mg |
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| Other Sizes |
| Targets |
Multiple targets: inflammatory mediators; immune cells; antioxidant pathways; cancer-related targets.
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|---|---|
| ln Vitro |
Lobetyolinin exhibits biological activities typical of polyacetylene glycosides, including anti-inflammatory, immunomodulatory, and antioxidant effects. It may inhibit the production of pro-inflammatory mediators and modulate immune cell function. The compound may also show antioxidant activity by scavenging free radicals. Anticancer activity has been reported in some polyacetylene compounds.
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| ln Vivo |
In vivo studies of lobetyolinin are limited. Codonopsis pilosula has been used in traditional Chinese medicine for its immunomodulatory and tonic effects. Lobetyolinin and related polyacetylenes contribute to the pharmacological activities of Codonopsis. Further studies are needed to characterize the specific in vivo activities of lobetyolinin.
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| Enzyme Assay |
Anti-inflammatory activity is assessed by measuring the inhibition of NO production, cytokine levels, or COX-2/iNOS expression in enzyme assays or cell-based systems. Antioxidant activity is assessed by DPPH, ABTS, or FRAP assays. Immunomodulatory activity is assessed by measuring lymphocyte proliferation, cytokine production, or macrophage function.
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| Cell Assay |
Macrophage cell lines (e.g., RAW 264.7) are stimulated with LPS and treated with lobetyolinin at various concentrations. Inflammatory mediators in culture supernatant are measured. For immunomodulatory studies, splenocytes or peripheral blood mononuclear cells are treated with the compound and proliferation or cytokine production is measured. Antioxidant activity is measured in cell-free or cell-based assays.
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| Animal Protocol |
In vivo studies for lobetyolinin are not well documented. Potential animal models include: carrageenan-induced paw edema for anti-inflammatory evaluation; and immunosuppression models for immunomodulatory evaluation. Standard protocols for these models involve administration of the compound followed by measurement of relevant endpoints.
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| ADME/Pharmacokinetics |
Pharmacokinetic data for lobetyolinin are limited. As a glycoside, it is expected to be metabolized to release the aglycone. The compound is soluble in organic solvents. Standard laboratory storage conditions apply. It is intended for research use only.
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| Toxicity/Toxicokinetics |
Specific toxicity data for lobetyolinin are limited. Standard laboratory safety practices should be followed when handling this compound. It is intended for research use only.
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| References | |
| Additional Infomation |
Lobetyolinin is a polyacetylene glycoside isolated from Codonopsis pilosula (Dangshen), a traditional Chinese medicinal herb used for its immunomodulatory and tonic effects. Polyacetylenes are a class of natural products with diverse biological activities, including anti-inflammatory, immunomodulatory, antioxidant, and anticancer effects. Lobetyolinin is used as a reference standard for quality control of Codonopsis-containing products and for research purposes. It is for research use only.
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| Molecular Formula |
C26H38O13
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|---|---|
| Molecular Weight |
558.57
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| Exact Mass |
558.231
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| CAS # |
142451-48-7
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| PubChem CID |
5459227
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| Appearance |
White to off-white solid
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| Density |
1.48g/cm3
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| Boiling Point |
891.7ºC at 760mmHg
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| Flash Point |
493.1ºC
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| Vapour Pressure |
0mmHg at 25°C
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| Index of Refraction |
1.633
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| LogP |
-2.7
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| Hydrogen Bond Donor Count |
9
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| Hydrogen Bond Acceptor Count |
13
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| Rotatable Bond Count |
12
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| Heavy Atom Count |
39
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| Complexity |
927
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| Defined Atom Stereocenter Count |
0
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| SMILES |
O1C([H])([C@@]([H])([C@]([H])([C@@]([H])([C@@]1([H])C([H])([H])O[C@@]1([H])[C@@]([H])([C@]([H])([C@@]([H])([C@@]([H])(C([H])([H])O[H])O1)O[H])O[H])O[H])O[H])O[H])O[H])OC([H])(/C(/[H])=C(\[H])/C([H])([H])C([H])([H])C([H])([H])O[H])C([H])(C#CC#C/C(/[H])=C(\[H])/C([H])([H])[H])O[H]
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| InChi Key |
GDLSOFWVVAOUJI-FWTOVJONSA-N
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| InChi Code |
InChI=1S/C26H38O13/c1-2-3-4-5-7-10-15(29)16(11-8-6-9-12-27)37-26-24(35)22(33)20(31)18(39-26)14-36-25-23(34)21(32)19(30)17(13-28)38-25/h2-3,8,11,15-35H,6,9,12-14H2,1H3/b3-2+,11-8+
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| Chemical Name |
2-[[6-[(4E,12E)-1,7-dihydroxytetradeca-4,12-dien-8,10-diyn-6-yl]oxy-3,4,5-trihydroxyoxan-2-yl]methoxy]-6-(hydroxymethyl)oxane-3,4,5-triol
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| Synonyms |
Lobetyolinin
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.7903 mL | 8.9514 mL | 17.9029 mL | |
| 5 mM | 0.3581 mL | 1.7903 mL | 3.5806 mL | |
| 10 mM | 0.1790 mL | 0.8951 mL | 1.7903 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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