| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
|
||
| Other Sizes |
|
| ln Vivo |
In WT mice, plsminogen (plg) (2 mg/per; iv; once daily for 16 days) speeds up the healing of burn injuries[1]. In the injured skin of both WT and plg-deficient mice, plsminogen (plg) (2 mg/per; iv; single day for 16 days) increases the production of IL-6 and augments the activation of STAT3[1]. In a diabetic mouse model, plsminogen (plg) (2 mg/per; iv; once daily for 24 days) enhances the healing of burn wounds[1].
|
|---|---|
| Animal Protocol |
Animal/Disease Models: WT mice (plg-heterozygous (plg+/-)) mice, plg+/- and plg-deficient (plg-/-) mice (C57BL/6 background; 8- to 10- week-old; burn-wound model)[1].
Doses: 2 mg/per Route of Administration: intravenous (iv) injection; single daily for 16 days. Experimental Results: demonstrated a Dramatically faster healing speed than control group at day 6, and the time to healing (ie, the scab falling off) was also approximately 2 days earlier than in the control group. Promoted epithelium layer fused to reepithelialize the wound completely, and only a small scab remained lightly attached above the wound when at day 11. Enhanced the level of IL-6 in the wounds of both WT and plg-deficient mice, and increased the pSTA T3 level in the wound. Animal/Disease Models: Genetically diabetic mice (C57BLKS db/db; at least 10 weeks old; with a minimal blood glucose level of 15 mM) and control heterozygous littermates (C57BLKS db/+; at least 10 weeks old; with a minimal blood glucose level of 7.8 mM)[1]. Doses: 2 mg/per Route of Administration: Intrav |
| References |
[1]. Shen Y, et al. Plasminogen is a key proinflammatory regulator that accelerates the healing of acute and diabetic wounds. Blood. 2012 Jun 14;119(24):5879-87.
[2]. Keragala CB, et al. Plasminogen: an enigmatic zymogen. Blood. 2021 May 27;137(21):2881-2889. |
| CAS # |
9001-91-6
|
|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
|---|
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
