| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| Other Sizes |
| Targets |
Sialic acid synthases and sialyltransferases; cell surface receptors involved in immune recognition. 9-Amino-NeuAc is a substrate for sialic acid synthases and can be converted to CMP-9-amino-NeuAc, the activated form required for incorporation into glycoconjugates by sialyltransferases. Once incorporated into cell surface glycoproteins and glycolipids, the modified sialic acid can modulate cell surface immunogenicity. Sialic acids play critical roles in cellular recognition and signaling processes. The mechanism of action involves interaction with specific receptors on cell surfaces. The compound's amino group allows for conjugation with other molecules, enabling targeted delivery and functional studies.
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| ln Vitro |
9-Amino-NeuAc is a substrate for sialic acid synthases and can be converted to CMP-9-amino-NeuAc, the activated form required for incorporation into glycoconjugates. The compound can be used to target liposome synthesis while modulating tumor surface immunogenicity. The amino group at the 9-position makes it a functionalized version of sialic acid, allowing for easier conjugation with other molecules, such as proteins or nanoparticles. The compound's ability to be incorporated into cell surface glycans enables studies of sialic acid function in cellular recognition and signaling.
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| ln Vivo |
In vivo studies of 9-amino-NeuAc are limited. The compound can be used to target liposome synthesis while modulating tumor surface immunogenicity. By incorporating the modified sialic acid into cell surface glycoconjugates, the compound may alter immune recognition of tumor cells. Sialic acids play critical roles in cellular recognition and signaling processes, and modifications to sialic acid structure can affect these functions. Further in vivo studies are needed to fully characterize the compound's pharmacokinetic and pharmacodynamic properties.
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| Enzyme Assay |
Non-cell-based assays for 9-amino-NeuAc include enzymatic assays to assess its substrate activity for sialic acid synthases and sialyltransferases. The conversion of 9-amino-NeuAc to CMP-9-amino-NeuAc can be measured using HPLC or radiometric assays. Binding to sialic acid-binding proteins such as siglecs can be assessed using surface plasmon resonance (SPR) or ELISA. For compound characterization, standard analytical methods including HPLC, NMR, and mass spectrometry are used to confirm identity and purity.
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| Cell Assay |
Cell-based assays for 9-amino-NeuAc use various cell lines to study its incorporation into cell surface glycoconjugates and its effects on cell function. Cells are cultured with 9-amino-NeuAc, and incorporation into glycoproteins and glycolipids is assessed using lectin binding, antibody detection, or metabolic labeling with fluorescent or radioactive probes. Effects on cell surface immunogenicity are assessed by measuring immune cell activation or binding of sialic acid-binding proteins. The compound's effects on tumor cell recognition by immune cells can be studied in co-culture assays.
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| Animal Protocol |
In vivo studies of 9-amino-NeuAc are limited. Potential applications include: tumor models for studying modulation of tumor surface immunogenicity; and models for studying sialic acid function in development and disease. The compound can be administered via various routes, and incorporation into tissue glycoconjugates can be assessed by mass spectrometry or lectin histochemistry. Further studies are needed to establish the compound's in vivo efficacy and safety.
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| ADME/Pharmacokinetics |
9-Amino-NeuAc has a molecular formula of C₁₁H₂₀N₂O₈ and a molecular weight of 308.29 g/mol. It is soluble in DMSO at 50 mg/mL (162.18 mM) with ultrasonic assistance. The compound should be stored at -20°C. It has a purity of ≥98%. The compound is a sialic acid derivative and is intended for research use only. It is not for human consumption. Specific storage and handling conditions should be followed as per the manufacturer's recommendations.
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| Toxicity/Toxicokinetics |
Specific toxicity data for 9-amino-NeuAc are limited. As a sialic acid derivative, it is expected to have low toxicity. Sialic acids are naturally occurring carbohydrates found in all mammalian cells. The compound is intended for research use only. Standard laboratory safety practices should be followed when handling this compound, including the use of personal protective equipment.
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| References |
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| Additional Infomation |
9-Amino-NeuAc (9-Amino-N-acetylneuraminic acid) is a C-9 modified derivative of N-acetylneuraminic acid (Neu5Ac), the primary sialic acid in mammals. Sialic acids are nine-carbon sugars that play critical roles in cellular recognition, signaling, and immune modulation. The amino group at the 9-position allows for conjugation with other molecules, enabling targeted delivery and functional studies. 9-Amino-NeuAc is a substrate for sialic acid synthases and can be incorporated into glycoconjugates via the sialic acid biosynthetic pathway. The compound can be used to target liposome synthesis while modulating tumor surface immunogenicity, making it of interest for cancer immunotherapy research. It is for research use only.
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| Exact Mass |
308.122
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|---|---|
| CAS # |
153232-60-1
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| PubChem CID |
124397065
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| Appearance |
Off-white to light yellow solid powder
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| Hydrogen Bond Donor Count |
7
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| Hydrogen Bond Acceptor Count |
9
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| Rotatable Bond Count |
5
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| Heavy Atom Count |
21
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| Complexity |
406
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| Defined Atom Stereocenter Count |
6
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| SMILES |
CC(=O)N[C@@H]1[C@H](C[C@](O[C@H]1[C@@H]([C@@H](CN)O)O)(C(=O)O)O)O
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: ~50 mg/mL (~162.2 mM; with ultrasonication)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 1.25 mg/mL (4.05 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), Clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (12.5 mg/mL) to 400 μL of PEG300 and mix well; then add 50 μL of Tween-80 and mix well; finally add 450 μL of physiological saline and adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 1.25 mg/mL (4.05 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (12.5 mg/mL) to 900 μL of 20% SBE-β-CD saline and mix well. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 1.25 mg/mL (4.05 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (12.5 mg/mL) to 900 μL of corn oil and mix well. (Please use freshly prepared in vivo formulations for optimal results.) |
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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