| Size | Price | Stock | Qty |
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| 1mg |
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| Other Sizes |
| Targets |
Multiple targets including cough reflex pathways, inflammatory mediators, and cancer cell signaling pathways. Isoverticine, as a steroidal alkaloid, exhibits antitussive, expectorant, anti-inflammatory, and anticancer activities. The compound may suppress the cough reflex, reduce inflammation, and inhibit cancer cell proliferation. Its mechanism of action may involve modulation of neurotransmitter receptors, inflammatory pathways, and cell cycle regulators.
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| ln Vitro |
With IC50s of 49.56, 31.63, 61.91, and 108.8 µg/mL, respectively, isoverticine exhibits cytotoxicity[1].
Isoverticine exhibits antitussive, expectorant, anti-inflammatory, and anticancer activities characteristic of Fritillaria alkaloids. It may suppress the cough reflex, reduce inflammation, and inhibit cancer cell proliferation. Its specific in vitro activities depend on the concentrations tested and the assay systems used. Further studies are needed to fully characterize its activity profile. |
| ln Vivo |
In vivo studies of isoverticine are limited. As a steroidal alkaloid from Fritillaria species, it may contribute to the pharmacological effects of Fritillaria extracts, which have been used in traditional medicine for respiratory conditions. Its antitussive, expectorant, and anti-inflammatory activities suggest potential benefits in respiratory diseases. Further in vivo studies are needed to characterize its specific pharmacokinetic and pharmacodynamic properties.
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| Enzyme Assay |
Non-cell-based assays for isoverticine include receptor binding assays for targets involved in cough reflex and inflammation. Standard analytical methods including HPLC, NMR, and mass spectrometry are used for compound characterization and purity assessment.
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| Cell Assay |
Cell-based assays for isoverticine use various cell lines to assess its biological activities. Inflammatory mediator production is measured in macrophage cell lines. Cancer cell proliferation and apoptosis are assessed in cancer cell lines.
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| Animal Protocol |
In vivo studies of isoverticine are limited. Based on its biological activities, potential animal models include: cough models for antitussive evaluation; carrageenan-induced paw edema for anti-inflammatory evaluation; and tumor xenograft models for anticancer evaluation. Standard protocols for these models involve administration of the compound followed by measurement of relevant endpoints.
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| ADME/Pharmacokinetics |
Isoverticine has a molecular formula of C₂₇H₄₅NO₃ and a molecular weight of approximately 431.65 g/mol. It is a naturally occurring steroidal alkaloid isolated from Fritillaria species. The compound is soluble in organic solvents such as DMSO and methanol. It should be stored under recommended conditions.
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| Toxicity/Toxicokinetics |
Specific toxicity data for isoverticine are limited. As a natural steroidal alkaloid, it may have biological activity at certain concentrations. Standard laboratory safety practices should be followed when handling this compound.
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| References | |
| Additional Infomation |
Reports indicate that isoviteline has been found in Fritillaria ebeiensis, Fritillaria thunbergii, and Fritillaria anhuiensis, and relevant data are available for reference.
Isoverticine is a naturally occurring steroidal alkaloid isolated from Fritillaria species, including Fritillaria verticillata and other medicinal plants. Steroidal alkaloids are known for their diverse biological activities including antitussive, expectorant, anti-inflammatory, and anticancer effects. Fritillaria species have been used in traditional medicine for respiratory conditions. Isoverticine is used as a reference standard for the identification and quantification of Fritillaria alkaloids and for research purposes. It is for research use only. |
| Molecular Formula |
C27H45NO3
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|---|---|
| Molecular Weight |
431.65
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| Exact Mass |
431.34
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| CAS # |
23496-43-7
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| PubChem CID |
21573744
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| Appearance |
White to off-white solid powder
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| LogP |
3.616
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
0
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| Heavy Atom Count |
31
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| Complexity |
715
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| Defined Atom Stereocenter Count |
13
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| SMILES |
C[C@H]1CC[C@H]2[C@@]([C@H]3CC[C@@H]4[C@H]([C@@H]3CN2C1)C[C@H]5[C@H]4C[C@H]([C@@H]6[C@@]5(CC[C@@H](C6)O)C)O)(C)O
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| InChi Key |
IUKLSMSEHKDIIP-RZNVUHDWSA-N
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| InChi Code |
InChI=1S/C27H45NO3/c1-15-4-7-25-27(3,31)21-6-5-17-18(20(21)14-28(25)13-15)11-22-19(17)12-24(30)23-10-16(29)8-9-26(22,23)2/h15-25,29-31H,4-14H2,1-3H3/t15-,16-,17+,18+,19-,20-,21-,22-,23+,24+,25-,26+,27-/m0/s1
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| Chemical Name |
(1R,2S,6S,9S,10S,11S,14S,15S,17R,18S,20S,23R,24S)-6,10,23-trimethyl-4-azahexacyclo[12.11.0.02,11.04,9.015,24.018,23]pentacosane-10,17,20-triol
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 100 mg/mL (231.67 mM)
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.3167 mL | 11.5835 mL | 23.1669 mL | |
| 5 mM | 0.4633 mL | 2.3167 mL | 4.6334 mL | |
| 10 mM | 0.2317 mL | 1.1583 mL | 2.3167 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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