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| 10mg |
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| Targets |
Anti-T7 tag antibodies (for detection and purification). T7 Tag Peptide is a protein tag derived from the T7 bacteriophage capsid protein gp10 that is specifically recognized by T7 tag antibodies. It does not have a biological target but serves as an affinity tag for the detection and purification of recombinant proteins.
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| ln Vitro |
T7 Tag Peptide is used as an affinity tag for the detection and purification of recombinant proteins. It is specifically recognized by T7 tag antibodies in immunoassays. The peptide itself does not exhibit pharmacological activity.
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| ln Vivo |
In vivo studies of T7 Tag Peptide are not applicable as it is a protein tag used for in vitro detection and purification of recombinant proteins.
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| Enzyme Assay |
T7 Tag Peptide is used in immunoassays and affinity purification applications. Standard protocols involve using the peptide as a fusion tag for recombinant proteins, which are then detected or purified using anti-T7 tag antibodies.
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| Cell Assay |
T7 Tag Peptide is used in cell-based assays as a fusion tag for recombinant proteins expressed in cells. The tagged proteins can be detected or purified using anti-T7 tag antibodies in immunoassays.
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| Animal Protocol |
In vivo animal studies for T7 Tag Peptide are not applicable as the compound is a protein tag used for in vitro research applications.
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| ADME/Pharmacokinetics |
T7 Tag Peptide has a molecular formula of C₄₁H₇₁N₁₃O₁₆S₃ and a molecular weight of 1098.27 g/mol. The sequence is H-Met-Ala-Ser-Met-Thr-Gly-Gly-Gln-Gln-Met-Gly-OH. It is a protein tag derived from the T7 bacteriophage capsid protein gp10. It is used in immunoassays and affinity purification.
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| Toxicity/Toxicokinetics |
T7 Tag Peptide is generally considered safe for research use. Standard laboratory safety practices should be followed when handling this compound.
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| References | |
| Additional Infomation |
T7 Tag Peptide is a protein tag peptide composed of 11 residues from the N-terminus of the T7 bacteriophage capsid protein gp10. It is specifically recognized by T7 tag antibodies and can be used in immunoassays and affinity purification. The peptide can be fused to recombinant proteins for detection and purification purposes. It is for research use only.
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| Molecular Formula |
C41H71N13O16S3
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|---|---|
| Molecular Weight |
1098.27494
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| Exact Mass |
1097.43
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| CAS # |
245445-88-9
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| Related CAS # |
T7 Tag Peptide TFA
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| PubChem CID |
170907469
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| Appearance |
White to off-white solid powder
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| Hydrogen Bond Donor Count |
16
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| Hydrogen Bond Acceptor Count |
20
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| Rotatable Bond Count |
38
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| Heavy Atom Count |
73
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| Complexity |
1920
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| Defined Atom Stereocenter Count |
9
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| SMILES |
C[C@H]([C@@H](C(=O)NCC(=O)NCC(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CCSC)C(=O)NCC(=O)O)NC(=O)[C@H](CCSC)NC(=O)[C@H](CO)NC(=O)[C@H](C)NC(=O)[C@H](CCSC)N)O
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| InChi Key |
CBOSLARKSDSTLV-AQKXWGSKSA-N
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| InChi Code |
InChI=1S/C41H71N13O16S3/c1-20(48-35(64)22(42)10-13-71-3)34(63)53-27(19-55)40(69)52-26(12-15-73-5)39(68)54-33(21(2)56)41(70)46-16-30(59)45-17-31(60)49-23(6-8-28(43)57)37(66)50-24(7-9-29(44)58)38(67)51-25(11-14-72-4)36(65)47-18-32(61)62/h20-27,33,55-56H,6-19,42H2,1-5H3,(H2,43,57)(H2,44,58)(H,45,59)(H,46,70)(H,47,65)(H,48,64)(H,49,60)(H,50,66)(H,51,67)(H,52,69)(H,53,63)(H,54,68)(H,61,62)/t20-,21+,22-,23-,24-,25-,26-,27-,33-/m0/s1
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| Chemical Name |
2-[[(2S)-2-[[(2S)-5-amino-2-[[(2S)-5-amino-2-[[2-[[2-[[(2S,3R)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-amino-4-methylsulfanylbutanoyl]amino]propanoyl]amino]-3-hydroxypropanoyl]amino]-4-methylsulfanylbutanoyl]amino]-3-hydroxybutanoyl]amino]acetyl]amino]acetyl]amino]-5-oxopentanoyl]amino]-5-oxopentanoyl]amino]-4-methylsulfanylbutanoyl]amino]acetic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O: 1.79 mg/mL (1.63 mM)
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 0.9105 mL | 4.5526 mL | 9.1051 mL | |
| 5 mM | 0.1821 mL | 0.9105 mL | 1.8210 mL | |
| 10 mM | 0.0911 mL | 0.4553 mL | 0.9105 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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